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2 protocols using anti c myc

1

Antibody Reagents for Protein Analysis

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Anti-Flag (F1804; Sigma-Aldrich), anti-Arid5a (customized antibody; Sigma-Aldrich), anti–Regnase-1 (sc-515275; Santa Cruz Biotechnology), anti-KPNA2 (ab84440; Abcam), anti–Lamin-B (PM064; MBL), anti–α-tubulin (M175; MBL), anti-UPF1 (sc-166092; Santa Cruz Biotechnology), anti-UPF2 (D3B10; Cell Signaling Technology), anti-UPF3A (MBS712426; My Bio Source), anti-UPF3B (HPA001882; Atlas), and anti–c-Myc (Nacalai Tesque) antibodies were used in this study.
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2

Comprehensive Neuronal Cell Marker Profiling

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The following primary antibodies were used for indirect immunofluorescence staining: anti-Cux1 (sc-13024, Santa Cruz Biotechnology); anti-Ctip2 (ab18465, Abcam); anti- GFP (GFP-1010, Aves Labs); anti-Pax6 (PD022, MBL); anti-bromodeoxyuridine (BrdU; catalog #555627, BD Biosciences); anti-phospho-histone-H3 (Ser10; catalog #09-797, Millipore); anti-Rapgef2 (Wei et al., 2007 (link)); anti-Rapgef6 (Yoshikawa et al., 2007 (link)); anti-β-catenin (catalog #610153, BD Biosciences); anti-N-cadherin (catalog #610920, BD Biosciences); anti-E-cadherin (catalog #610181, BD Biosciences); anti-afadin (catalog #ab90809, Abcam); anti-ZO-1 (catalog #61-7300, Life Technologies); anti-nestin (catalog #556309, BD Biosciences); anti-Tbr1 (catalog #ab31940, Abcam); and anti-c-Myc (catalog #23941-54, Nacalai Tesque). To detect immunoreactive signals, the following secondary antibodies were used: Alexa Fluor 488-conjugated anti-chicken Ig Y (catalog #703-545-155, Jackson ImmunoResearch); Alexa Fluor 647-conjugated anti-rabbit IgG (catalog #A21244, Life Technologies); CF647-conjugated anti-mouse IgG (catalog #20281, Biotium); CF488A-conjugated anti-IgG (catalog #20302, #20015, #20027, Biotium); CF488A anti-mouse IgG (Biotium); and CF555-conjugated anti-IgG (catalog #20231, #20038, #20233, Biotium). An Alexa Fluor 647-conjugated anti-Tbr2 antibody (catalog #51-4875, eBioscience) was also used.
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