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Duosets

Manufactured by Thermo Fisher Scientific
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Duosets are a set of matched antibody pairs designed for the development of sandwich ELISAs. They provide the basic components needed to measure specific proteins in a variety of sample types. The Duosets include a capture antibody, a detection antibody, and a standard.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) Siglecf pe, by BD (2 mentions) Cd40 pe, by BD (2 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions) Gr1 fitc, by BD (2 mentions) Livedead aqua or blue, by Thermo Fisher Scientific (2 mentions) Cd4 apc or a700, by BD (2 mentions) Cd19 e780, by BD (2 mentions) Cd44 bv570, by BD (2 mentions) Cd69 fitc cd86 af488, by BD (2 mentions) Foxp3 e450, by BD (2 mentions) Hucd2 pe, by BD (2 mentions) Igm apce780, by BD (2 mentions) Tcrβ apce780, by BD (2 mentions) Cd80 percp cy5, by BD (2 mentions) F4 80 pe cy7, by BD (2 mentions) Cd11b bv711, by BD (2 mentions) Cd8α pe cy7, by BD (2 mentions) Cd3 apce780, by BD (2 mentions) Facs lsr 2, by BD (1 mentions)

Close spelling variants of this product

Duoset ELISA kit (2 citations) DuoSet! ELISA (2 citations) Mouse IL-6 and TNF-α DuoSet ELISA kit (2 citations)

2 protocols using duosets

1

Multiparametric Flow Cytometry Analysis

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Cells were first stained with LiveDead aqua or blue (Life Technologies). Following FcR-Block (2.4G2), cells were stained using the following monoclonal antibodies (all used at 1:200 dilution): CD3-APCe780, CD4-APC or A700, CD8α-PE/Cy7, CD11c-APC or BV421, MHC-II-PerCP/Cy5.5 or eFlour450, CD11b-BV711 or PE, CD19-e780, CD80-PerCP/Cy5.5, CD40-PE, CD44-BV570, CD69-FITC CD86-AF488, Foxp3-e450, F4/80-PE/Cy7, Gr1-FITC, HuCD2-PE, IgM-APCe780, SiglecF-PE and TCRβ-APCe780 (BD Biosciences, BioLegend or eBioscience). Foxp3 staining was performed with the eBioscience FoxP3 staining kit. Samples were acquired using FACS LSR II or FACS Canto II using BD FACSDiva software and analysed with FlowJo v.9 software (Tree Star). Cytokines were measured in culture supernatants by ELISA using paired monoclonal antibody, and recombinant cytokine standards, or Duosets (eBioscience, BD Biosciences, BioLegend, R&D Systems and Peprotech).
Cook P.C., Owen H., Deaton A.M., Borger J.G., Brown S.L., Clouaire T., Jones G.R., Jones L.H., Lundie R.J., Marley A.K., Morrison V.L., Phythian-Adams A.T., Wachter E., Webb L.M., Sutherland T.E., Thomas G.D., Grainger J.R., Selfridge J., McKenzie A.N., Allen J.E., Fagerholm S.C., Maizels R.M., Ivens A.C., Bird A, & MacDonald A.S. (2015). A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells. Nature Communications, 6, 6920.
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2

Flow Cytometry Immunophenotyping Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were first stained with LiveDead aqua or blue (Life Technologies). Following FcR-Block (2.4G2), cells were stained using the following mAb (all used at 1:200 dilution): CD3-APCe780, CD4- APC or A700, CD8α-PE/Cy7, CD11c- APC or BV421, MHCII-PerCP/Cy5.5 or eFlour450, CD11b-BV711 or PE, CD19-e780, CD80-PerCP/Cy5.5, CD40-PE, CD44-BV570, CD69-FITC CD86-AF488, Foxp3-e450, F4/80-PE/Cy7, Gr1-FITC, HuCD2-PE, IgM-APCe780, SiglecF-PE and TCRβ-APCe780 (BD Biosciences, BioLegend, or eBioscience). Foxp3 staining was performed with the eBioscience FoxP3 staining kit. Samples were acquired using FACS LSR II or FACS Canto II using BD FACSDiva software and analyzed with FlowJo v.9 software (Tree Star). Cytokines were measured in culture supernatants by ELISA using paired mAb, and recombinant cytokine standards, or Duosets (eBioscience, BD Biosciences, BioLegend, R&D Systems, and Peprotech).
Cook P.C., Owen H., Deaton A.M., Borger J.G., Brown S.L., Clouaire T., Jones G.R., Jones L.H., Lundie R.J., Marley A.K., Morrison V.L., Phythian-Adams A.T., Wachter E., Webb L.M., Sutherland T.E., Thomas G.D., Grainger J.R., Selfridge J., McKenzie A.N., Allen J.E., Fagerholm S.C., Maizels R.M., Ivens A.C., Bird A, & MacDonald A.S. (2015). A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells. Nature Communications, 6, 6920.
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