Easycyte flow cytometer system

1 × 10 6 MDA-MB-453 cells treated with 177 Lu-CHX-A"-DTPA-trastuzumab and unlabeled CHX-A"-DTPAtrastuzumab were harvested after trypsinization and resuspended in 100 μL of 0.05 M PBS, pH 7.4 containing 1% BSA. To this, Guava nexin reagent (100 μL) was added and incubated in dark for 20 min at RT. Samples were acquired on a Guava EasyCyte Flow Cytometer System and analyzed with Guava Soft 3.1.1.

1 × 10 6 MDA-MB-453 cells were plated overnight in DMEM medium containing 10% FBS. The adherent cells were grouped in triplicates and given treatments as mentioned above and incubated for a period of 48 h at 37°C in a humidified 5% CO 2 atmosphere. Equivalent number of MDA-MB-453 cells without any treatment served as the control group. After the incubation period, cells were harvested by trypsinization and resuspended in 50 μL of PBS containing 1% bovine serum albumin (BSA). 250 μL of Guava via count reagent was added into each tube and incubated in dark for 15 min at room temperature (RT). Samples were acquired on a Guava EasyCyte Flow Cytometer System and analyzed with guava Soft 3.1.1.