Rabbit anti s aureus polyclonal igg

Herculase II Fusion DNA
polymerase was obtained from Agilent (Seoul, Korea). Oligonucleotides
were obtained from Bionics (Seoul, Korea). The plasmid miniprep kit
was obtained from GeneAll Biotechnology (Seoul, Korea). Protein A
beads were obtained from GE healthcare (Piscataway, NJ). A disposable
gravity column was obtained from Bio-Rad (Daejeon, Korea). Ultrafiltration
devices were obtained from Millipore (centrifugal filter tube Ultra-4,
MWCO 3k; Seoul, Korea). The 96-well Maxi-binding ELISA plate was obtained
from SPL (Seoul, Korea). Freestyle 293 medium was obtained from Gibco
(Waltham, MA). PEI was obtained from Polysciences (Warrington, PA).
LTA was obtained from Sigma (Seoul, Korea). Goat anti-mouse IgG HRP
was obtained from Pierce (Seoul, Korea). Mouse anti-S. aureus monoclonal IgM and rabbit anti-S. aureus polyclonal IgG were obtained from Thermo.
HRP-conjugated goat anti-rabbit IgG (H+L) and HRP-conjugated goat
anti-mouse IgM were obtained from Invitrogen (Waltham, MA). Other
chemicals and reagents, unless otherwise indicated, were from Sigma.

Chemical gene was synthesized from LncBio (Seoul, Korea). Oligonucleotides and the plasmid miniprep kits were obtained from Bionics (Seoul, Korea). KOD-One DNA polymerase and the In-Fusion HD cloning kit were from Takara-Bio (Tokyo, Japan). E. coli SHuffle T7 Express lysY were obtained from New England Biolabs (Seoul, Korea). Talon beads were obtained from Clontech (Mountain View, CA, USA). The gravity empty column was from Bio-Rad (Daejeon, Korea). Ultrafiltration devices were obtained from Pall (Nanosep Centrifugal-3k; Port Washington, NY, USA). HRP-conjugated anti-DYKDDDDK antibody was obtained from (Biolegend, CA, USA). Spin-type ultrafiltration device was obtained from Millipore (Bedford, MA, USA). Rabbit anti-S. aureus polyclonal IgG and rabbit anti-P. aeruginosa polyclonal IgG were obtained from Thermo (Waltham, Massachusetts, USA). Alexa647–NHS ester was obtained from Broad Pharm (San Diego, USA). Hydroxyl amine-HCl was obtained from Daejung (Cheongju, Korea).