Cd8 rpa t8 fitc

Manufactured by BioLegend

CD8(RPA-T8)-FITC is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen on the surface of T cells. It is a useful tool for the identification and enumeration of CD8+ T cells in flow cytometry applications.

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Lab products found in correlation

Negative selection microbeads kit, by Miltenyi Biotec (2 mentions) Cd4 okt4 bv510, by BioLegend (2 mentions) Cd3 percp ucht1, by BioLegend (2 mentions) Facsmelody, by BD (2 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD4 PE-Cy7 (SK3); CD8 PerCP-Cy5.5 (RPA-T8); CCR7 FITC (G043H7); CD45RA BV421 (HI100); HLA-DR PE (L243); CD38 BV605 (HIT2) RPA-T8 CD8-FITC

2 protocols using cd8 rpa t8 fitc

Isolating and Characterizing CMV-Specific CD8+ T Cells

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CD8⁺ T cells were isolated from PBMCs using a negative selection microbeads kit (Miltenyi Biotec) according to the manufacturer's protocol. Next, CD8⁺ T cells were labeled at room temperature for 20 min with the A^*0201/GLCTLVAML-APC dextramer and with the A^*0201/NLVPMVATV-APC dextramer for CMV⁺ individuals. Subsequently surface staining was performed using the following mAbs: CD3(UCHT1)-PerCP (Biolegend), CD4(OKT4)-BV510 (Biolegend), and CD8(RPA-T8)-FITC (Biolegend). CD3⁺CD4⁻CD8⁺dextramer⁺ cells were then sorted by FACS Melody (BD) directly into RNAlater (Ambion Inc. Applied Biosystems) and stored at −80°C for subsequent TCRβ clonotype analysis.

Lanfermeijer J., de Greef P.C., Hendriks M., Vos M., van Beek J., Borghans J.A, & van Baarle D. (2021). Age and CMV-Infection Jointly Affect the EBV-Specific CD8+ T-Cell Repertoire. Frontiers in Aging, 2, 665637.

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Isolation and Characterization of GILG-Specific CD8+ T Cells

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CD8⁺ T cells were isolated from PBMCs using a negative selection microbeads kit (Miltenyi Biotec). Next, CD8⁺ T cells were labeled at room temperature for 20 minutes with the A*0201/GILGFVFTL-APC dextramer (Immudex) (GILG). Subsequently surface staining was added with the following mAbs: CD3(UCHT1)-PerCP (Biolegend), CD4(OKT4)-BV510 (Biolegend) and CD8(RPA-T8)-FITC (Biolegend) and CD3⁺CD4^-CD8⁺GILG⁺ cells were sorted by FACS Melody (BD) directly into RNAlater (Ambion Inc. Applied Biosystems) and stored at -80°C for subsequent TCRβ clonotype analysis.

van den Berg S.P., Lanfermeijer J., Jacobi R.H., Hendriks M., Vos M., van Schuijlenburg R., Nanlohy N.M., Borghans J.A., van Beek J., van Baarle D, & de Wit J. (2021). Latent CMV Infection Is Associated With Lower Influenza Virus-Specific Memory T-Cell Frequencies, but Not With an Impaired T-Cell Response to Acute Influenza Virus Infection. Frontiers in Immunology, 12, 663664.

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