Hydrogen peroxide

Manufactured by Vetec

Sourced in Brazil

Hydrogen peroxide is a chemical compound with the formula H2O2. It is a colorless liquid with a slightly pungent odor. Hydrogen peroxide is a strong oxidizing agent and can be used for a variety of laboratory applications.

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Lab products found in correlation

5 protocols using hydrogen peroxide

Graphene-based Biosensor for COVID-19 Antibody Detection

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Sulfuric acid, ethanol, isopropanol, monobasic sodium phosphate (NaH₂PO₄) and potassium phosphate were acquired from Synth®, Brazil. Hydrochloric acid (37% v/v), toluene, polystyrene (MW ∼192,000 g mol^-1), tetrachloroauric acid trihydrate, ethanolamine, Tween 20, potassium chloride, neutral red dye and bovine serum albumin protein were bought from Sigma Aldrich®. Acetone was purchased from Chemis®, Brazil, and hydrogen peroxide (30–32%, v/v) was acquired from Vetec®, Brazil. Monoclonal IgG human antibodies (IgG1 type) from SARS-CoV-2 (ab273073) were obtained from Abcam®, USA. Human IgM antibody was acquired from Rheabiotech, Brazil. Bilirubin oxidase was purchased from Amano, Japan. For graphene devices confection, p-doped Si/SiO₂ (

Φ_{{SiO}_{2}}

= 90 nm) were purchased from Graphene Supermarket®, USA. CVD monolayer graphene was acquired from Graphenea®, Spain. Metallic spots for electrical contact films deposition of Ti and Pt were obtained from Electron Microscopy Sciences®, USA. All aqueous solutions were made using deionized water with resistivity >18 MΩ cm.

Mattioli I.A., Castro K.R., Macedo L.J., Sedenho G.C., Oliveira M.N., Todeschini I., Vitale P.M., Ferreira S.C., Manuli E.R., Pereira G.M., Sabino E.C, & Crespilho F.N. (2021). Graphene-based hybrid electrical-electrochemical point-of-care device for serologic COVID-19 diagnosis. Biosensors & Bioelectronics, 199, 113866.

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Quantitative Catalase Activity Assay

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Catalase (CAT) activity was quantified according to Sudhakar et al. (2001) (link). Briefly, the reaction was initiated by adding the enzymatic extract (50 µL) to the quartz cuvette containing reaction medium (2.95 mL) composed of potassium phosphate buffer (final concentration of 50 mM and final pH of 7) (Monobasic-NEON©, Brazil and Dibasic-NEON, Brazil) and mixed with hydrogen peroxide (VETEC©, Brazil) (20 mM). Then, after slight agitation, the mixture was taken to a spectrophotometer where the reduction of absorbance at 240 nm was monitored for 1.5 min, with readings taken every 15 s. The Lambert-Beer equation (A = ε.b.c), where A = decrease in absorbance (triplicate mean); ε = molar extinction coefficient; b = optical path length; and c = concentration of the enzyme expressed as mol L -1 , was used. The molar extinction coefficient of hydrogen peroxide is 39.4 mol -1 cm -1 . Final CAT activity was expressed in µmol H 2 O 2 g FM -1 min -1 .

Andrade W.L.d., Melo A.S.d., Melo Y.L., Sá F.V.d.S., Rocha M.M., Oliveira A.P.d.S., & Fernandes‐Júnior P.I. (2020). Bradyrhizobium Inoculation Plus Foliar Application of Salicylic Acid Mitigates Water Deficit Effects on Cowpea. Journal of Plant Growth Regulation, 40(2), 656-667.

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Phytochemical and Antioxidant Assays

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We purchased 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), acetone, acetonitrile, citric acid, DPPH, ferrous-ion-o-dianisidine, Folin–Ciocalteu phenol reagent, formic acid, gallic acid, hydrochloric acid, methanol, β-nicotinamide adenine dinucleotide 2-phosphate reduced tetrasodium salt (NADPH), ultrapure phenolic standards, 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald), sodium acetate, superoxide dismutase determination kit, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ascorbic acid, boric acid, ferric chloride, ferrous sulfate, hydrogen peroxide, potassium chloride, sodium carbonate, sodium hydroxide, and sulfuric acid were obtained from Vetec (Rio de Janeiro, RJ, Brazil). Cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, delphinidin, quercetin-3-O-glucoside, 3-O-caffeoyl-quinic acid, and myricetin were purchased from Extrasynthese (Genay, France). HA, PCA, VA, isovanillic acid (iVA), ellagic acid, FA, and kaempferolwere purchased from Sigma-Aldrich (St Louis, MO, USA). The kit to analyze urine creatinine was purchased from Labtest Diagnóstica S.A. (Lagoa Santa, MG, Brazil).

Cardoso A.L., Teixeira L.D., Hassimotto N.M., Baptista S.D., Copetti C.L., Rieger D.K., Vieira F.G., Micke G.A., Vitali L., de Assis M.A., Schulz M., Fett R., da Silva E.L, & Pietro P.F. (2023). Kinetic Profile of Urine Metabolites after Acute Intake of a Phenolic Compounds-Rich Juice of Juçara (Euterpe edulis Mart.) and Antioxidant Capacity in Serum and Erythrocytes: A Human Study. International Journal of Molecular Sciences, 24(11), 9555.

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Biochemical Analysis of Plant Compounds

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Catechol (1,2-dihydroxybenzene), rutin, cyanidin chloride and bovine serum albumin V (BSA) were from SigmaeAldrich (St. Louis, MO, USA). Tannic acid, hydrogen peroxide and Folin-Ciocalteu reagent were from Vetec Química Fina Ltda (Duque de Caxias, RJ, Brazil). All other reagents were of analytical grade and solutions were prepared with distilled water.

Siqueira B.d.S., Bassinello P.Z., Santos S.C., Malgaresi G., Ferri P.H., Rodriguez A.G, & Fernandes K.F. (2016). Do enzymatic or non-enzymatic pathways drive the postharvest darkening phenomenon in carioca bean tegument?. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 69.

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Antioxidant and Anti-Inflammatory Assays

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Dexamethasone (DEXA), 12-O-tetradecanoylphorbol-acetate (TPA), hexadecyl-trimethylammonium bromide (HTAB), tetramethylbenzidine (TMB) all from Sigma Chemcil Co (St Louis MO); acetone, propanone, formaldehyde, glacial acetic acid, paraffin, dimethylformamide, phosphate-buffered (PBS) 2′,7′-dichlorodihydrofluorescein diacetate (DCHF-DA), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ascorbic acid were purchased from Merck Biosciences (Germany); hydrogen peroxide, sodium acetate, ethanol, hematoxylin, and eosin were purchased from Vetec (Rio de Janeiro, Brazil). The other chemical reagents of analytical grade were purchased from local chemical suppliers.

Ascari J., de Oliveira M.S., Nunes D.S., Granato D., Scharf D.R., Simionatto E., Otuki M., Soley B, & Heiden G. (2019). Chemical composition, antioxidant and anti-inflammatory activities of the essential oils from male and female specimens of Baccharis punctulata (Asteraceae). Journal of ethnopharmacology, 234.

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