Log In Sign up
The largest database of trusted experimental protocols

Sds page reducing buffer

Manufactured by Thermo Fisher Scientific
Visit Supplier

SDS-PAGE reducing buffer is used to prepare protein samples for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. It contains the reducing agent dithiothreitol (DTT) or beta-mercaptoethanol to disrupt disulfide bonds and denature proteins, allowing for their separation by molecular weight.

Automatically generated - may contain errors

Lab products found in correlation

Abi 7900ht, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions)

Close spelling variants of this product

SDS-PAGE (402 citations) Reducing buffer (12 citations)

2 protocols using sds page reducing buffer

1

Quantitative Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular RNA was extracted by RNeasy kit (Qiagen) followed by reverse
transcription (Qiagen) according to the manufacturer's instruction. Quantitative
PCR (qPCR) was performed using SYBR green PCR master mix and ABI 7900HT (Applied
Biosystems). Human tata-box binding protein (hTBP) was used as internal control and the
primers for all tested genes were listed in Supplementary Table 2. The relative gene
expression was calculated as described previously 61 . Cellular proteins were extracted using RIPA buffer (Life
Technologies) and dissolved in SDS-PAGE reducing buffer (Life Technologies). Immunoblots
were developed using rabbit anti-human NRP1 antibody and mouse anti-β actin
antibody. IRDye secondary antibodies were used to detect the primary antibodies and the
blots were imaged with Odyssey (Li-Cor).
Pang H.B., Braun G.B., Friman T., Aza-Blanc P., Ruidiaz M.E., Sugahara K.N., Teesalu T, & Ruoslahti E. (2014). An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability. Nature communications, 5, 4904.
+ Open protocol
+ Expand
2

Quantitative Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular RNA was extracted by RNeasy kit (Qiagen) followed by reverse
transcription (Qiagen) according to the manufacturer's instruction. Quantitative
PCR (qPCR) was performed using SYBR green PCR master mix and ABI 7900HT (Applied
Biosystems). Human tata-box binding protein (hTBP) was used as internal control and the
primers for all tested genes were listed in Supplementary Table 2. The relative gene
expression was calculated as described previously 61 . Cellular proteins were extracted using RIPA buffer (Life
Technologies) and dissolved in SDS-PAGE reducing buffer (Life Technologies). Immunoblots
were developed using rabbit anti-human NRP1 antibody and mouse anti-β actin
antibody. IRDye secondary antibodies were used to detect the primary antibodies and the
blots were imaged with Odyssey (Li-Cor).
Pang H.B., Braun G.B., Friman T., Aza-Blanc P., Ruidiaz M.E., Sugahara K.N., Teesalu T, & Ruoslahti E. (2014). An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability. Nature communications, 5, 4904.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!