G-Rh2 was purchased from Chengdu Phytoelite Bio-Technology Co., Ltd. (Chengdu, Sichuan, China) and the purity (>98%) was determined by high-performance liquid chromatography (Figure 1 ). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Annexin V-FITC apoptosis detection kit were purchased from Sigma-Alorich (St. Louis, MO, United States). The fetal bovine serum (FBS) was purchased from Corning (Medford, MA, United States). Antibiotics (100× penicillin/streptomycin) and 0.25% Trypsin-EDTA were purchased from Gibco (California, United States). Minimum essential medium (MEM) was purchased from Hyclone (Logan, UT, United States). RIPA Lysis Buffer and eECL Western Blot kit were purchased from CWBio (Taizhou, Jiangsu, China). The primary antibodies against p-AKT, AKT, p-PDK1, PDK1, p-p85, p85, p-IκBα, IκBα, and anti-rabbit IgG HRP (#7074) were from Cell Signaling Technology (Danvers, MA, United States).
Antibiotics 100 penicillin streptomycin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Antibiotics (100× penicillin/streptomycin) is a sterile liquid solution containing 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin in a 0.85% sodium chloride solution. It is commonly used as a supplementary component in cell culture media to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
P akt, by Cell Signaling Technology (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
Eecl western blot kit, by CWBIO (1 mentions)
Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
P pdk1, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by CWBIO (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Faststart universal sybr green master, by Roche (1 mentions)
Superoxide dismutase sod assay kit, by Nanjing Jiancheng (1 mentions)
2 nbdg, by Thermo Fisher Scientific (1 mentions)
Cell lysis buffer, by CWBIO (1 mentions)
Eecl kit, by CWBIO (1 mentions)
Donkey anti rabbit igg h l alexa fluor 647, by Abcam (1 mentions)
Malondialdehyde mda, by Nanjing Jiancheng (1 mentions)
2 protocols using antibiotics 100 penicillin streptomycin
1
G-Rh2 Cytotoxicity and Apoptosis Assays
2
Cellular Signaling Pathway Analysis
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GF2 (purity,≥99%) was purchased from Chengdu PhytoElite Biotech (Chengdu, China). The MEM medium was obtained from Hyclone (Logan, UT, USA). Antibiotics (100 × penicillin/streptomycin) and Trypsin-EDTA were purchased from Gibco (California, USA). Fetal bovine serum (FBS) was purchased from Corning (New Zealand). 2-NBDG and TRIzol reagent were from Invitrogen (Carlsbad, CA, USA). cDNA synthesis kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). FastStart Universal SYBR Green Master was provided from Roche (Mannheim, Germany). Malondialdehyde (MDA) and superoxide dismutase (SOD) assay kits were acquired form Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). The kits for reactive oxygen species assay, glycogen PAS stain and glycogen content assay were purchased from Solarbio (Beijing, China). Cell lysis buffer and eECL kit were purchased from CWBio (Jiangsu, China). The primary antibodies against p-JNK (#4671), JNK (#9258), p-ERK1/2 (#8544), ERK1/2 (#4348), p-p38 MAPK (#4511), p38MAPK (#9212), NF-κB p65 (#8242), p-AKT (#4060), AKT (#9272), p-PDK1 (#3438), PDK1 (#3062), p-GSK-3β (#5558), GSK3β (#12456), Anti-β-actin pAb-HRP-DirecT (#PM053-7) was from Medical & Biological Laboratories CO., LTD (Nagoya, Japan). Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075) was from Abcam (Cambridge, MA, USA).
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