Facs calibur ow cytometry system

Upon treatment with different concentrations of ALT, both BV173 and NALM6 cells were harvested and washed with PBS for 3 times. For cell apoptosis analysis, cells were labeled with Annexin V-Fluorescein Isothiocyanate (FITC)/propidium iodide (PI) according to manufacturer's instruction. Finally, cells were observed and photographed with the BD FACS Calibur ow cytometry system (Becton Dickinson, NJ, USA).
JC-1 staining JC-1 staining was performed to assess the mitochondrial membrane potential (MMP) using a JC-1 assay kit (Beyotime, Shanghai, China) according to the operating instruction. Images were taken under a uorescence microscope (Leica, Wetzlar, Germany). The ratio (%) of red/green uorescence intensity was calculated by Image J software.

Upon treatment with different concentrations of Alantolactone, both BV173 and NALM6 cells were harvested and washed with PBS for 3 times. For cell apoptosis analysis, cells were labeled with Annexin V-Fluorescein Isothiocyanate (FITC) /propidium iodide (PI) according to manufacturer's instruction. Finally, cells were observed and photographed with the BD FACS Calibur ow cytometry system (Becton Dickinson, NJ, USA).