Microsyringe

Manufactured by Hamilton Company

Sourced in United States, Switzerland

The Microsyringe is a precision laboratory instrument used for the accurate and controlled delivery of small volumes of liquids. It features a fine-tipped needle and a calibrated plunger mechanism that allows for the precise measurement and dispensing of micro-scale quantities. The core function of the Microsyringe is to facilitate the handling and transfer of minute liquid samples in various scientific and analytical applications.

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Lab products found in correlation

Rompun, by Bayer (4 mentions) C57bl 6j male mice, by Jackson ImmunoResearch (2 mentions) 30 gauge needle, by Hamilton Company (2 mentions) Nylon mesh filters, by Avantor (2 mentions) 26 gauge needle, by Hamilton Company (2 mentions) Microinfusion pump, by KD Scientific (2 mentions) Optical fiber, by Doric (2 mentions) Ksv 2000 langmuir balance, by Biolin Scientific (2 mentions) Micron 4 retinal imaging microscope, by Phoenix Pharmaceuticals (1 mentions) 33g needle, by Hamilton Company (1 mentions) Lps from e coli 055 b5, by Merck Group (1 mentions) Aav nc, by Genechem (1 mentions) Legato 130, by KD Scientific (1 mentions) Paav hsyn dio hm3d gq mcherry, by Addgene (1 mentions) Ftir 8400, by Shimadzu (1 mentions) Quanta 250 feg, by Thermo Fisher Scientific (1 mentions) Stereotaxic apparatus, by Narishige (1 mentions) Male icr mice, by Charles River Laboratories (1 mentions) Tc10 automated cell counter, by Bio-Rad (1 mentions) Microinfusion pump, by Harvard Apparatus (1 mentions)

34 protocols using microsyringe

Chick Embryo Vascular Morphology Assay

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We used CAM assay to investigate
the vascular morphology and angiogenic molecules using metabolomics
approaches including mass spectrometry analysis.^{83 (link)−85 (link)} Fertilized
eggs of white Leghorn chickens were purchased from Veterinary Research
Institute Jos and incubated for 1–21 days at 37.5 °C with
62–72% humidity in a laboratory incubator with continuous rotation.
Chick embryos were incubated for each day (ED1–ED21) and harvested
in Petri dishes.
For lipid addition experiments, both dose-dependent
effects and time-dependent vessel growth were examined. Cholesterol
(55, 110 mg) or oleic acid (45, 200 mg) in PBS/dimethyl sulfoxide
was added after preincubation day 2 using a microsyringe (200 μL
capacity, Hamilton Company, Bonaduz, Switzerland) through the hole
(0.2 mm diameter) made with forceps. Solvent toxicity was validated
by the negative control (vehicle only). After lipid addition, the
chick embryo was sealed with 3M transparent scotch tape and further
incubated for 1–4 days followed by harvest and vessel analysis.

Samson F.P., Patrick A.T., Fabunmi T.E., Yahaya M.F., Madu J., He W., Sripathi S.R., Tyndall J., Raji H., Jee D., Gutsaeva D.R, & Jahng W.J. (2020). Oleic Acid, Cholesterol, and Linoleic Acid as Angiogenesis Initiators. ACS Omega, 5(32), 20575-20585.

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Selective Astrocyte Ablation and Microglia Inactivation

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In order to selectively abrogate astrocytes, the astrocytic toxin L-α-aminoadipate (20 μg/μl stock, 2 μl at a constant rate of 0.4 μl/min) was infused into the bilateral hippocampus (Lima et al., 2014 (link)). The animals were anesthetized with isoflurane (5% induction, 2% maintenance) and fixed on a stereotactic platform (RWD Life Science, San Diego, CA, United States). The stereotactic coordinates for the positioning of the guide cannula into the bilateral hippocampus were −2 mm anterior-posterior, ± 1.5 mm lateral to bregma, and 1.5 mm below the surface of the skull. A micro-syringe (5 μl; Hamilton Company, Reno, NV, United States) was used for injection, and constant rate infusion was delivered by a stereotactic injection syringe pump (RWD Life Science). At the end of the infusion, the dummy cannula was retained at the injection place to allow sufficient diffusion. To selectively inactivate microglia, mice were injected intraperitoneally (i.p.) with minocycline (40 mg/kg; Bassett et al., 2021 (link)). Animals were decapitated and their hippocampal slices were prepared the 3rd day after either pre-treatment.

Zhang Y., Yin H.Y., Rubini P., Illes P, & Tang Y. (2022). ATP indirectly stimulates hippocampal CA1 and CA3 pyramidal neurons via the activation of neighboring P2X7 receptor-bearing astrocytes and NG2 glial cells, respectively. Frontiers in Pharmacology, 13, 944541.

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Intravitreal AAV Delivery in Mice

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Mice were anaesthetised using intraperitoneal injection of 90 μL/10 g body weight of a solution of Ketavet (Ketamine hydrochloride 100 mg/mL; Zoetis Ireland Ltd.) and Rompun (Xylazine hydrochloride 20 mg/mL; Bayer PLC) mixed with sterile water in the ratio of 0.6:1:8.4 respectively. Pupils were dilated with a single drop of 1% w/v Tropicamide (Chauvin Pharmaceuticals) prior to injection. 2μL volume of titer-matched AAV at 5 × 10¹² vg/mL was delivered into the intravitreal space via the pars plana, using an operating microscope and a 33-gauge needle on a microsyringe under direct visualisation (Hamilton Company). The contralateral eye of each animal received a control injection of PBS. Immediately following injection, 1% Chloramphenicol ointment (Martindale Pharma, Wooburn Green, UK) was applied topically, and the animals were warmed on a heat-pad for recovery. Laterality of injected eyes was randomized, and investigators were masked to the vector type throughout intervention and analysis.
On selected days post-injection (dpi), pupils were dilated, and mice anaesthetised using isofluorane 2% (v/v) by inhalation for clinical assessment. The Micron IV retinal imaging microscope (Phoenix Research Laboratories) was used to capture OCT scans and color and fluorescence fundal images.

Chan Y.K., Wang S.K., Chu C.J., Copland D.A., Letizia A.J., Verdera H.C., Chiang J.J., Sethi M., Wang M.K., Neidermyer WJ J.r., Chan Y., Lim E.T., Graveline A.R., Sanchez M., Boyd R.F., Vihtelic T.S., Inciong R.G., Slain J.M., Alphonse P.J., Xue Y., Robinson-McCarthy L.R., Tam J.M., Jabbar M.H., Sahu B., Adeniran J.F., Muhuri M., Tai P.W., Xie J., Krause T.B., Vernet A., Pezone M., Xiao R., Liu T., Wang W., Kaplan H.J., Gao G., Dick A.D., Mingozzi F., McCall M.A., Cepko C.L, & Church G.M. (2021). Engineering adeno-associated viral vectors to evade innate immune and inflammatory responses. Science translational medicine, 13(580), eabd3438.

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Locust Reproductive Pathway Manipulation

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Adult male locusts were injected with 1 µg dsRNA (in 6 µL S. gregaria Ringer solution) targeting Scg-Met (n = 25) or Scg-Tai (n = 20) (1 L Ringer solution: 8.766 g NaCl; 0.188 g CaCl₂; 0.746 g KCl; 0.407 g MgCl₂; 0.336 g NaHCO₃; 30.807 g sucrose; 1.892 g trehalose; pH 7.2). For all injections, the needle of the micro-syringe (Hamilton Company, Reno, NV, USA) was inserted laterally between the second and third abdominal segments and oriented towards the animal’s anterior side. The corresponding control groups were injected with 1 µg dsGFP (n = 25 in dsScg-Met experiment and n = 20 in dsScg-Tai experiment). Injections were initiated at the first day after the adult molt (day 0) and were repeated every 3 days to ensure a potent and persistent knockdown of the targets. The injections were pursued until day 34 after the adult molt. Then animals were sacrificed to collect their testes, AG, CA, fat body, and epidermis (originating from the ventral side of the third abdominal segment).

Holtof M., Van Lommel J., Gijbels M., Dekempeneer E., Nicolai B., Vanden Broeck J, & Marchal E. (2021). Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts. Biomolecules, 11(2), 244.

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Anesthesia Protocol for Intravitreal Injections in Mice

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Mice were anesthetized using an intraperitoneal injection of 90 μL/10 g body weight of a solution of Ketavet (ketamine hydrochloride 100 mg/mL; Zoetis Ireland, Dublin, Ireland) and Rompun (xylazine hydrochloride 20 mg/mL; Bayer PLC, Newbury, UK) mixed with sterile water in the ratio of 0.6:1:8.4, respectively.
All intravitreal injections used were 2 μL in volume at the titers indicated in the text and were delivered using an operating microscope and a 33G needle on a microsyringe under direct visualization (Hamilton Company, Reno, NV, USA). 1% Chloramphenicol ointment (Martindale Pharma, Wooburn Green, UK) was applied topically immediately following injection. Pupils were dilated with a single drop of 1% w/v Tropicamide (Chauvin Pharmaceuticals, Romford, UK) prior to fluorescent retinal imaging or OCT of corneal and retinal thickness using the Micron IV platform (Phoenix Research Laboratories, Pleasanton, CA, USA).

Wu J., Bell O.H., Copland D.A., Young A., Pooley J.R., Maswood R., Evans R.S., Khaw P.T., Ali R.R., Dick A.D, & Chu C.J. (2020). Gene Therapy for Glaucoma by Ciliary Body Aquaporin 1 Disruption Using CRISPR-Cas9. Molecular Therapy, 28(3), 820-829.

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Intravitreal LPS Injection in Mice

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Prior to anesthesia, pupils were dilated using topical tropicamide 1% w/v and phenylephrine 2.5% w/v (Minims; Chauvin Pharmaceuticals, Romford, UK). Mice were anaesthetised by intraperitoneal injection of 90 μL/10 g body weight of a solution containing 6 mg/mL ketamine (Ketavet; Zoetis Ireland Ltd., Dublin, Ireland) and 2 mg/mL Xylazine (Rompun; Bayer plc, Newbury, UK) mixed with sterile water.
Mice were selected for injection (or to be used as a control) in a constrained randomised order within blocks using Excel 2016 (Microsoft, Redmond, WA); blocks were dependent on cage allocations, which itself was dependent on the litter they were derived from. The allocations ensured that all experiments had littermate controls.
Intravitreal injections were performed as previously described (22 (link)). In brief, 2 μL volume of PBS containing 10 ng LPS from E. coli 055:B5 (Sigma-Aldrich) was delivered into the intravitreal space via the pars plana, using an operating microscope and a 33-gauge needle on a microsyringe (Hamilton Company, Reno, NV) under direct visualisation. Immediately following injection, 1% w/w chloramphenicol ointment (Martindale Pharma, Romford, UK) was applied topically, with the animals monitored and kept on a heat-pad during recovery.

Bell O.H., Copland D.A., Ward A., Nicholson L.B., Lange C.A., Chu C.J, & Dick A.D. (2020). Single Eye mRNA-Seq Reveals Normalisation of the Retinal Microglial Transcriptome Following Acute Inflammation. Frontiers in Immunology, 10, 3033.

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Intrasheath Injection Optimization in Mice

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To determine the feasibility and optimal parameters of intrasheath injection, mice were injected with varying volumes of Toluidine Blue (TB) dye (1μL, 2μL, and 5μL). Successful injections were evaluated by the efficacy of TB dye localization to the tendon sheath with minimal detection of the TB dye in the surrounding tissue (Supplemental Figure 1). Thirty-six 14-week old C57BL/6J male mice were purchased from Jackson Laboratories (Stock #0664). Animals were housed on a 12-hour light/dark cycle and were ad libitum fed standard chow. Animals were administered pre-operative analgesia (1 mg/kg of Sustained-Release Buprenorphine), and were sedated with 100mg/kg Ketamine, via intraperitoneal injection. Postoperative pain monitoring was conducted for 3 days after each injection. A microsyringe (#7635-01, Hamilton Company, Reno, Nevada) with a 30-gauge needle (#7803-07, Hamilton Company) was filled with 2μL of either 1x10 7 CFU Xen29 or sPBS. The needle was inserted into the medial aspect of the third digit on the right hindpaw between the proximal interphalangeal joint (PIP) and the metatarsophalangeal (MTP) joint, utilizing forceps for stabilization of the third digit. This digit was chosen because it is the longest and largest digit so as to provide the highest likelihood of entering the sheath.

Qiu B., Cobb J., Loiselle A.E, & Ketonis C. (2021). Development of a Murine Model of Pyogenic Flexor Tenosynovitis. The Journal of bone and joint surgery. American volume, 103(5).

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SEM, FTIR and Contact Angle Analysis of CMS

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CMS were characterized for their morphology and elemental analysis using SEM (SEM-JEOL JSM-IT 5300, Japan), for their organic functional groups using FTIR (Model 111653, Liantrisant, UK) and contact angle by measuring the hydrophilicity of the prepared membrane surfaces (Ramé hart, Instrument Company, France) to determine their contact angles. A drop of distilled water (2 μL) was placed on the RO membrane surface (3 × 2 cm) using a micro syringe (Hamilton Company, Reno, NV). The contact angle was calculated as an average of the measurement at 5 different positions within 20 s. after placing the water drop on the membrane surface.

El Bestawy E., El-Hameed A.S, & Fadl E. (2024). Desalination of seawater using integrated microbial biofilm/cellulose acetate membrane and silver NPs/activated carbon nanocomposite in a continuous mode. Scientific Reports, 14, 274.

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Orthotopic 4T1 Mammary Tumor Model in Mice

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Only female mice were used in all experiments. The 4T1 mammary carcinoma cell line was a gift from Xiao-Fan Wang (Duke University). Tumor cells were harvested by trypsinization, and cell viability was evaluated by trypan blue exclusion. 100 4T1 cells in 10 μL of serum-free media were orthotopically injected directly into the mammary gland of anesthetized Female BALB/c mice using a micro-syringe with a 26-gauge needle (Hamilton Company, Reno, NV). Tumor progression was monitored closely and tumor growth kinetics were measured. Mice were sacrificed at three weeks post tumor injection for all tumor-cell sorting experiments and their tumors, tumor mammary mucosa, and contralateral (distant) mammary mucosa tissue were harvested. The primary tumor was first removed, followed by dissection of the remaining mucosa tissue surrounding the tumor (tumor mucosa). Care was taken during the dissection process to ensure that the inguinal lymph nodes were removed prior to mammary mucosa tissue harvest. All tissues were mechanically homogenized and filtered over 70 μm nylon mesh filters (VWR) to obtain a single cell suspension for downstream assays. Enzymatic digestion was avoided as to eliminate the possibility that antibody binding sites could be degraded.

Christian L.S., Wang L., Lim B., Deng D., Wu H., Wang X.F, & Li Q.J. (2021). Resident memory T cells in tumor-distant tissues fortify against metastasis formation. Cell reports, 35(6), 109118.

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Intrahippocampal Protein Injection in Mice

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Five-month-old male C57BL/6JNarl mice or APP/PS1ΔE9 male mice were anaesthetized by intraperitoneal injection of a mixture of tranquilizer (Zoleti 50, 1 mg/10 mg body weight, Vibrac, Amherst, MA, USA), analgesics (Rompun, 10 μL/10 mg body weight, Bayer, Toronto, Canada). The C57BL/6JNarl mice received bilateral intrahippocampal injection of 2 μL recombinant Aβ40 or Aβ42 (25 μM), full-length TDP-43 (0.25 μM), or TDP-43-induced Aβ (25 μM Aβ40 or Aβ42 with 0.25 μM TDP- 43). APP/PS1ΔE9 mice received bilateral intrahippocampal injection of 2 μL recombinant full-length TDP-43 (2.5 μM). The stereotaxic coordinates of the injection site are in relation to bregma as follows: Posterior 2 mm; mediolateral 1 mm; ventral 2 mm. The injection needle was slowly approached to the desired depth and the mentioned protein was injected using a microsyringe (0.1 μL/min, 32-gauge Hamilton Company, NV, USA). The needle left in place for an additional 5 min to limit the diffusion of the injected protein.

Shih Y.H., Tu L.H., Chang T.Y., Ganesan K., Chang W.W., Chang P.S., Fang Y.S., Lin Y.T., Jin L.W, & Chen Y.R. (2020). TDP-43 interacts with amyloid-β, inhibits fibrillization, and worsens pathology in a model of Alzheimer’s disease. Nature Communications, 11, 5950.

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