Fluorescent microscope

Manufactured by Keyence

Sourced in Japan

The Fluorescent microscope is a specialized imaging instrument used to observe and analyze fluorescent samples. It utilizes a light source that excites fluorescent molecules within the sample, causing them to emit light at a different wavelength. This allows for the visualization and study of specific cellular structures, proteins, or other fluorescently labeled components within a sample.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

BZ-X710 fluorescent microscope (46 citations) BZ-X700 fluorescent microscope (35 citations) BZ-9000 fluorescent microscope (25 citations) BZ-X800 fluorescent microscope (15 citations) BZ-X810 fluorescent microscope (10 citations) BZ-X700 all-in-one fluorescent microscope (9 citations) BIOREVO BZ-9000 fluorescent microscope (8 citations) BZ‐9000 fluorescent microscope system (6 citations) BioREVO fluorescent microscope (5 citations) BZ-X800E fluorescent microscope (4 citations)

23 protocols using fluorescent microscope

Comet Assay Protocol for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols

Comet assay kits were purchased from Trevigen, and neutral comet assay was performed according to the manufacturer’s instructions. Images were collected with a Keyence Fluorescent Microscope (Keyence America) and quantitated with OpenComet software.

Tsai C.Y., Sun S., Zhang H., Local A., Su Y., Gross L.A., Rice W.G, & Howell S.B. (2018). APTO-253 Is a New Addition to the Repertoire of Drugs that Can Exploit DNA BRCA1/2 Deficiency. Molecular cancer therapeutics, 17(6), 1167-1176.

+ Open protocol

+ Expand

Mucus and Immune Cell Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Periodic-acid Schiff (PAS; Sigma Aldrich, Saint Louis, MO) staining was used to evaluate mucus accumulation and immune cell infiltration. The Periodic-acid Schiff staining was performed following the manufacturer’s instructions. All images were taken at 20x magnification using Keyence Fluorescent Microscope (Keyence, Itasca, IL).

Han H., Peng G., Meister M., Yao H., Yang J.J., Zou M.H., Liu Z.R, & Ji X. (2021). Electronic Cigarette Exposure Enhances Lung Inflammatory and Fibrotic Responses in COPD Mice. Frontiers in Pharmacology, 12, 726586.

+ Open protocol

+ Expand

Microfluidic Assay for Immune Cell Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols

A PDMS microfluidics slide chip (Abnova) was coated with 1 mg/mL streptavidin (Agilent). The chip was then washed and coated with 0.4 mg/mL biotin-vimentin (MD Anderson) for 1 h. Next, 1.5 × 10⁶ attIL12-PBMCs (labeled with Calcein Green AM [ThermoFisher]) and 1.5 × 10⁶ control-PBMCs (labeled with CMTPX red cell tracker [ThermoFisher]) were mixed at a ratio of 1:1, loaded into a spiral chamber (Abnova), and passed through the slide chip at a flow rate of 1.8 mL per hour using the CytoQuest microfluidics pump (Abnova). The slide chip was then imaged on a fluorescent microscope (Keyence). Cells were counted using Keyence BZ-X700 analysis software.

, & Doolittle R.F. (1995). Of archae and eo: what's in a name?. Proceedings of the National Academy of Sciences of the United States of America, 92(7), 2421-2423.

+ Open protocol

+ Expand

Quantifying Proliferation during Adipogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

EdU incorporation was assessed using the Click-iT EdU Imaging Kit (Thermo, C10340) according to manufacturer’s recommendation. Briefly, 3T3-L1 were grown to confluency in a 96well cell imaging plate (Eppendorf, 0030741013) and kept at confluency for 2 days.
Differentiation was initiated using the differentiation cocktail indicated in the figure legend, and EdU was added for a final concentration of 1μM after 16h of differentiation. After a total of 40h of differentiation, cells were fixed in 4% PFA/PBS for 15min at room temperature, followed by 2 rinses in 3%BSA/PBS. Cell were permeabilized in 0.5% Triton-X/PBS for 20min at room temperature, followed by 2 rinses in 3%BSA/PBS. Samples were then incubated in freshly made Click-iT reaction cocktail for 30min at room temperature, followed by 1 rinse in 3%BSA/PBS and 1 rinse in PBS. Samples were then incubated in 2μg/ml Dapi in PBS for 30min at room temperature followed by 1 rinse in PBS. Samples were stored in PBS and images were acquired using a Keyence fluorescent microscope (10x objective). EdU positivity was assessed using the CellProfiler pipeline.

Hilgendorf K.I., Johnson C.T., Mezger A., Rice S.L., Norris A.M., Demeter J., Greenleaf W.J., Reiter J.F., Kopinke D, & Jackson P.K. (2019). Omega-3 fatty acids activate ciliary FFAR4 to control adipogenesis. Cell, 179(6), 1289-1305.e21.

+ Open protocol

+ Expand

Collagen and Elastin Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin skin sections were stained with collagen 1, collagen 3, and Elastin antibodies (Protein Tech, USA) followed by staining with fluorescent-labeled secondary antibodies. DAPI (Sigma, USA) was employed as a nuclear stain. Stained sections were imaged using a fluorescent microscope (Keyence, Japan).

Chinnapaka S., Yang K.S., Surucu Y., Bengur F.B., Arellano J.A., Tirmizi Z., Malekzadeh H., Epperly M.W., Hou W., Greenberger J.S., Rubin J.P, & Ejaz A. (2023). Human adipose ECM alleviates radiation-induced skin fibrosis via endothelial cell-mediated M2 macrophage polarization. iScience, 26(9), 107660.

+ Open protocol

+ Expand

Reactive Oxygen Species Quantification in Nematodes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

L1 larvae of wild-type (N2) and CF1038 transgenic strains were treated with different concentrations of VVE extract in S-medium for 48 h. After treatment, ROS production was quantified by the DCFH-DA method according to our previous work (15 (link), 17 (link)). The 50-μM DCFH-DA was added into S-medium and incubated in the dark at 20°C for 1 h.
Worm images were examined under a fluorescent microscope (Keyence Deutschland GmbH, Neu-Isenburg, Germany) at least 30 worms per group. The relative fluorescence of the whole body was examined using ImageJ software (National Institutes of Health, Bethesda, MD). The results are presented as mean fluorescence ± SEM.

Duangjan C., Rangsinth P., Zhang S., Gu X., Wink M, & Tencomnao T. (2021). Vitis Vinifera Leaf Extract Protects Against Glutamate-Induced Oxidative Toxicity in HT22 Hippocampal Neuronal Cells and Increases Stress Resistance Properties in Caenorhabditis Elegans. Frontiers in Nutrition, 8, 634100.

+ Open protocol

+ Expand

FISH Analysis of GLP-1R, GRIN1A, and GluA1 in LC Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used FISH to examine Glp1r, Grin1A, and GluA1 mRNA transcripts within DBH-expressing LC neurons. FISH provides high cellular resolution and is necessary here, as the GLP-1R antibody is notorious for its lack of specificity (121 (link)). Rats (n = 4) were anesthetized, and brains were removed and snap-frozen in hexane. Brains were coronally sectioned into three serial sets on a cryostat at 20 μm and mounted onto subbed slides before being placed in a vacuum chamber overnight. Tissue was processed according to the RNAscope Fluorescent Multiplex Assay kit protocol for fresh frozen tissue (Advanced Cell Diagnostics). Serial set 1 was processed with probes for Glp1r and DBH, serial set 2 was processed for Grin1A and DBH, and serial set 3 was processed for GluA1 and DBH. All probes were specific to the rat (Advanced Cell Diagnostics). Slides were coverslipped using Fluoro-Gel with 4′,6-diamidino-2-phenylindole (DAPI; Electron Microscopy Sciences). Images were obtained on a fluorescent microscope (Keyence) using the protocol described above.

Fortin S.M., Chen J.C., Petticord M.C., Ragozzino F.J., Peters J.H, & Hayes M.R. (2023). The locus coeruleus contributes to the anorectic, nausea, and autonomic physiological effects of glucagon-like peptide-1. Science Advances, 9(38), eadh0980.

+ Open protocol

+ Expand

Click-iT EdU Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell proliferation assay was performed using Click‐iT™ EdU Cell Proliferation Imaging Kit (C10340, Thermo Fisher). The cells were plated in 96‐well plates at 7 × 10³ cells, treated for 2 h at 37°C with 50 mol/L EdU solution, fixed for 30 min using 4% paraformaldehyde, and treated with Triton X‐100 (Thermo Fisher) for 10 min. The cells were reacted for 30 min with Click‐iT reaction solution in the dark before nuclear staining using Hoechst33342 solution (Beyotime). The cell proliferation rate was assessed using the images taken under a fluorescent microscope (Keyence).

Feng L., Yan Q., Pan H, & Shi W. (2023). METTL3 enhances the effect of YTHDF1 on NEDD1 mRNA stability by m6A modification in diffuse large B‐cell lymphoma cells. Immunity, Inflammation and Disease, 11(2), e789.

+ Open protocol

+ Expand

PDMS Microfluidics for T-Cell Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols

A PDMS microfluidics slide chip (Abnova) was coated with 1 mg/mL streptavidin (Agilent). The chip was then washed and coated with 4 mg/mL rmFGL2 (5257-FL, R&D Systems) conjugated with biotin for 1 h. Following this, 1.5 million T-Ctr cells (dyed green) and 1.5 million T-αFGL2 cells (dyed red) were loaded into a spiral chamber (Abnova) and passed through the slide chip at a flow rate of 1.8 mL per hour using the Cytoquest microfluidics pump (Abnova). The slide chip was then imaged on a fluorescent microscope (Keyence).

Zhao Q., Hu J., Kong L., Jiang S., Tian X., Wang J., Hashizume R., Jia Z., Fowlkes N.W., Yan J., Xia X., Yi S.F., Dao L.H., Masopust D., Heimberger A.B, & Li S. (2023). FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells. Nature Communications, 14, 735.

+ Open protocol

+ Expand

DNA Synthesis in Asynchronous Cell Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA synthesis in asynchronous cell populations was measured by incorporation of EdU, using a commercially available kit (Click-It EdU; Invitrogen Life Technologies, Carlsbad, CA) according to the manufacturer’s protocol. Briefly, cells were plated in triplicate on coverslips, pulsed with 10 μM EdU for 30 min at 37 °C, washed, labelled with Alexa Fluor 594, counterstained with 4,6-diamidino-2-phenylindole (DAPI), mounted and imaged using a fluorescent microscope (Keyence Corporation, Itasca, IL). The percentage of EdU-positive cells (EdU labelling index) was calculated by counting the number of Alexa Fluor 594-stained cells in two ×200 fields for each of three triplicate coverslips and dividing by the total cell number per field, expressed as a percentage. Experiments were repeated twice, and data were pooled for statistical analysis.

Shahoumi L.A., Khodadadi H., Bensreti H., Baban B, & Yeudall W.A. (2020). EPS8 phosphorylation by Src modulates its oncogenic functions. British Journal of Cancer, 123(7), 1078-1088.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!