Example 23
Blood pressure and heart rate were measured continuously throughout the experiment with a Spacelab monitor (Spacelabs Healthcare, Snoqualmie, Wash.). At baseline, at the end of blood removal (30 min) and at the end of the experiment (270 min), venous blood samples were drawn for analysis via blood gas analyzer (Gem Premier 3000, Instrumentation Laboratory Co, Bedford, Mass.) and to measure markers of organ function (including alkaline phosphatase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and urea nitrogen). Free plasma hemoglobin, a marker of in vivo hemolysis, was measured photometrically at the end of the shock period (30 min). Organ function markers and free plasma hemoglobin were analyzed in the Clinical Laboratory Improvement Amendments-certified Fairview Diagnostics Laboratory.