Wild-type dams were implanted with an infusion cannula (26 gauge, 5 mm, Plastics One, C315GAS-5/SPC) over the VTA. The day of the behavioural testing, 1 μl of the OXTR antagonist OTA (0.5 mg ml−1) or an equal volume of saline was infused over 2 min with a Pump 11 Elite Syringe Pump (Harvard Apparatus, HA1100). The pup-retrieval test started 5 min after infusion. Behavioural testing was performed on different days for OTA and saline.
Pump 11 elite syringe pump
Manufactured by Harvard Apparatus
Sourced in United States
The Pump 11 Elite Syringe Pump is a precision fluid delivery device. It is designed to accurately control the flow of liquids using a wide range of syringe sizes. The pump features programmable flow rates, selectable infusion and withdrawal modes, and a clear digital display.
Automatically generated - may contain errors
Lab products found in correlation
Labchart 7, by ADInstruments (2 mentions)
Dma 5000 m density meter, by Anton Paar (1 mentions)
Model fe221, by ADInstruments (1 mentions)
Surflo winged infusion set, by Terumo (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
27 gauge needle, by Terumo (1 mentions)
Emstat3, by PalmSens (1 mentions)
In vivo imaging system, by PerkinElmer (1 mentions)
Model 900, by Kopf Instruments (1 mentions)
Bridge amp ml 110, by ADInstruments (1 mentions)
Amplifier ml 785, by ADInstruments (1 mentions)
Powerlab 8sp, by ADInstruments (1 mentions)
11 protocols using pump 11 elite syringe pump
1
Oxytocin Receptor Antagonist Infusion
2
Microfluidic Production of Stable Foams
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A solution of 20 wt. % GM10, 0.14 wt. % LAP and 0.1 wt. % Plantacare 2000 UP was prepared with deionised water. The density of the solution was determined to be 1.056 g cm -3 by using a DMA 5000 M density meter from Anton Paar. All flasks were wrapped with aluminium foil and stored at 8 °C in the dark until further use to prevent early activation of the photo initiator.
Liquid foams were produced using a polycarbonate chip produced by micromilling, with a constriction of 70 µm in diameter (Figure 3). The flow of the gas phase was controlled by the gas pressure p. To this end, an OB1MK1 pressure controller from Elveflow was used, which was connected to a nitrogen tap. The pressure pump was also connected to a glass bottle containing a small amount of perfluorohexane. In this way, the gas phase contains traces of perfluorohexane, which hinders Ostwald ripening. The flow rate v of the liquid phase was controlled with a Pump 11 Elite Syringe Pump from Harvard Apparatus. Bubbling in the microfluidic chip was monitored with a Nikon SMZ 745 T bright field microscope using a Mikrotron EoSensCL high speed camera. The accessible range of bubble diameters that can be produced using this microfluidic chip was assessed by varying the gas pressure p and the liquid flow rate vL. The obtained data can be found in the Supporting Information (Figure S1).
Liquid foams were produced using a polycarbonate chip produced by micromilling, with a constriction of 70 µm in diameter (Figure 3). The flow of the gas phase was controlled by the gas pressure p. To this end, an OB1MK1 pressure controller from Elveflow was used, which was connected to a nitrogen tap. The pressure pump was also connected to a glass bottle containing a small amount of perfluorohexane. In this way, the gas phase contains traces of perfluorohexane, which hinders Ostwald ripening. The flow rate v of the liquid phase was controlled with a Pump 11 Elite Syringe Pump from Harvard Apparatus. Bubbling in the microfluidic chip was monitored with a Nikon SMZ 745 T bright field microscope using a Mikrotron EoSensCL high speed camera. The accessible range of bubble diameters that can be produced using this microfluidic chip was assessed by varying the gas pressure p and the liquid flow rate vL. The obtained data can be found in the Supporting Information (Figure S1).
3
Urodynamic Evaluation of Rat Bladders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urodynamic assessment and bladder capacity measurements were performed prior to bladder augmentation and euthanization as described31 (link). The bladders of anesthetized athymic nude rats were exposed through a lower abdominal incision. A 20 gauge cannula (Becton Dickinson, Franklin Lakes, NJ) was inserted into the bladder dome and connected to the Pump 11 Elite Syringe Pump (Harvard Apparatus, Holliston, MA) and to a physiological pressure transducer (SP844, MEMSCAP). The pressure transducer was connected to a bridge amplifier (Model FE221; AD Instruments, Colorado Springs, CO), which record and plotted continuous readings of the transvesical pressures using LabChart 7.3 Software (AD Instruments). Prior to filling, the bladder was manually decompressed to ensure that it was empty. The bladder was then filled at 150 µl/min. Bladder capacity was estimated by the product of flow rate and time to urethral leakage. Voiding pressure was the maximum pressure during the terminal contraction. Intravesical urodynamic measurements could not be performed at other timepoints of this study due to the highly invasive nature of the testing procedure.
4
Murine Intradermal and Subcutaneous Inoculation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six-to-eight week-old female C57BL/6J mice (Jackson Laboratory, Bar Harbor, ME) were inoculated under anesthesia (ketamine/xylazine). ID inoculations were done in the dorsal side of the ear pinna or the upper side of the foot. A volume of 2 μL was inoculated with the aid of a Pump11 Elite syringe pump (Harvard Apparatus, Holliston, MA) and a SURFLO winged infusion set with a 27-gauge needle (Terumo, Lakewood, CO). SC inoculations were performed as previously described [13 (link)] injecting a volume of 2 μL. Animals were sacrificed by injection with sodium pentobarbital. Organs were harvested at different time points and homogenized in PBS. Homogenates were serially diluted and plated on BHI agar and incubated at 26°C for 48 h to obtain bacterial counts. Mann Whitney or Wilcoxon matched pairs signed rank tests were used for statistical analysis, establishing statistical significance at p < 0.05 using GraphPad Prism version 4.0c (GraphPad Software, La Jolla, CA).
5
Lactate Sensor Evaluation in Artificial Sweat Glands
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fabricated lactate sensor
was evaluated via chronoamperometry (CA) using a potentiostat (EmStat3;
Palm Sens). The measurements were conducted in a 0.1 M phosphate buffer
solution with lactate concentrations ranging from 0 to 50 mM. For
chronoamperometry, a potential of +0.1 V vs Ag/AgCl was applied.
Artificial sweat glands, which are a microfluidic system that divides
a solution into four streams, were fabricated on a silicon wafer using
photoresist. A lactate sensor with a channel was affixed to the artificial
sweat gland using double-sided tape, and lactate was supplied through
the four inlets of the channel. The solution was delivered using a
syringe pump (Pump 11 Elite syringe pump, Harvard Apparatus) (Figure S1 ).
was evaluated via chronoamperometry (CA) using a potentiostat (EmStat3;
Palm Sens). The measurements were conducted in a 0.1 M phosphate buffer
solution with lactate concentrations ranging from 0 to 50 mM. For
chronoamperometry, a potential of +0.1 V vs Ag/AgCl was applied.
Artificial sweat glands, which are a microfluidic system that divides
a solution into four streams, were fabricated on a silicon wafer using
photoresist. A lactate sensor with a channel was affixed to the artificial
sweat gland using double-sided tape, and lactate was supplied through
the four inlets of the channel. The solution was delivered using a
syringe pump (Pump 11 Elite syringe pump, Harvard Apparatus) (
6
Porcine Intestine Bacterial Delivery
7
Protein Fiber Spinning Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fiber spinning protocol was adopted from previous publications with some modifications9 (link),10 (link),12 (link). Spinning dopes were prepared by the dissolution of lyophilized protein powders in HFIP. The resulting solution was loaded to a 100 μL Hamilton syringe (Hamilton Robotics, NV), and extruded into a 95% v/v methanol bath (pH = 8.0) using a Harvard Apparatus Pump 11 Elite syringe pump (Harvard Apparatus, MA) at a controlled protrusion rate of 10 μL/min. To achieve pH-controlled spinning, either 5 mM CH3COONa or 5 mM Na2CO3 was added to the 95% v/v methanol bath to adjust the pH to 5.5 or 11, respectively. The extruded fibers were then transferred to a 75-80% v/v methanol bath and extended to five times their original length. Following extension, the fibers were removed from the methanol bath and allowed to dry in a ventilated, dark, and dry room at room temperature until further testing.
8
Titin Fiber Spinning and Extension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fiber spinning was performed by first dissolving lyophilized titin powder in hexafluorisopropanol (HFIP) to 20% w/v. This protein dope was loaded to a 100 µL Hamilton gastight syringe (Hamilton Robotics) fitted with a 23s gauge (116 µm inner diameter and 4.34 cm length) needle. The syringe was fitted to a Harvard Apparatus Pump 11 Elite syringe pump (Harvard Apparatus), and the dope was extruded into a water bath at 5 µL/min. Short segments (~5–10 cm) were then cut from these extruded fibers and carefully extended by hand in water at approximately 1 cm/s to 5× their original length. Extended fibers were removed from the bath and held under tension until visibly dry.
9
Lateral Ventricle Cannulation in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ICP was manipulated via a custom made dual-cannula placed into the lateral ventricle on the side ipsilateral to the cannulated eye13 (link). The dual-lumen cannula consisted of a 23 G outer needle (0.6 mm diameter × 19 mm length, Becton Dickinson, Franklin, WI, USA) and a 30 G (0.3 mm diameter × 13 mm length, Becton Dickinson) inner needle, which were connect via polyethylene tubing (0.8 mm) to a pressure transducer (Transpac, Abbott Critical Care System) and a syringe pump (Pump 11 Elite Syringe Pumps, Harvard Apparatus, Holliston, MA, USA), respectively. This allowed for simultaneous ICP manipulation (Bridge Amp ML 110, Amplifier ML 785, Powerlab/8SP, ADInstruments) and recording (Lab Chart 7, ADInstruments). To prepare for lateral ventricle cannulation, rats were anesthetized and placed on a stereotaxic platform (Model 900, David Kopf Instruments, Los Angeles, CA, USA). A 2 cm by 2 cm flap of skin above the skull was removed. Connective tissue around the calvarial area was removed to expose the coronal sutures. Using a dental burr attached to a drill (Model 300, Dremel®, Robert Bosch Tool Corporation, Racine, WI, USA), a hole was drilled through the skull at 1.5 mm caudal to bregma and 2 mm lateral to the midline. The cannula was then inserted to a depth of 3.5 mm30 .
10
Intraspinal Microinjection of Zymosan A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non-traumatic intraspinal microinjection of Zymosan A was performed as previously described60 (link). Briefly, 50 nL Zymosan A (12.5 mg/ml, 273-01491, Wako) or 0.1 M PBS were injected into the lateral funiculi of spinal cords 1–1.2 mm lateral to the spinal cord midline and 0.5–0.7 mm deep at the level of T7/8. Injections were carried out over 5 min using calibrated pressure ejection (Harvard Apparatus, Pump 11 Elite Syringe Pumps). Mice were sacrificed at 3 days after injection and then analyzed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!