All human T-ALL cell lines (KOPT-K1, DND41, JURKAT, SUP-T13, HPB-ALL, MOLT-4, MOLT-16, RPMI-8402, TALL-1, and LOUCY) were obtained from ATCC (Manassas, VA, USA) or DSMZ (Braunschweig, Germany). The creation of Ba/F3 cells transformed by TYK2-E957D was described previously.6 (link) JURKAT and KOPT-K1 cells overexpressing BCL2 were generated with the MSCV-IRES-GFP retroviral expression system. JURKAT and KOPT-K1 cells overexpressing BCLXL or MCL1 cDNA were generated with the pHAGE-CMV-IRES-ZsGreen lentiviral expression system. For additional information, see Supplementary Materials and Methods . These cells were maintained in RPMI-1640 medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA).
Tall 1
Manufactured by American Type Culture Collection
Sourced in United States
The TALL-1 is a laboratory equipment designed to provide a stable and controlled environment for cell culture and other biological applications. It features precise temperature, humidity, and gas concentration regulation to support the optimal growth and maintenance of cell lines. The TALL-1 is a self-contained, vertical laminar flow unit that helps maintain a sterile and contaminant-free workspace.
Automatically generated - may contain errors
Lab products found in correlation
Jurkat, by American Type Culture Collection (4 mentions)
Kopt k1, by American Type Culture Collection (4 mentions)
Hpb all, by American Type Culture Collection (4 mentions)
Molt 4, by American Type Culture Collection (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Ccrf cem, by American Type Culture Collection (3 mentions)
Dnd41, by American Type Culture Collection (2 mentions)
Sup t13, by American Type Culture Collection (2 mentions)
Molt 16, by American Type Culture Collection (2 mentions)
Rpmi 8402, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated goat anti human igg, by Jackson ImmunoResearch (1 mentions)
Mm 1s, by American Type Culture Collection (1 mentions)
Facsdiva software, by BD (1 mentions)
Rpmi8226, by American Type Culture Collection (1 mentions)
Rs4 11, by American Type Culture Collection (1 mentions)
Nalm6, by American Type Culture Collection (1 mentions)
6 protocols using tall 1
1
Establishment and Maintenance of Human T-ALL Cell Lines
2
Establishment and Maintenance of Human T-ALL Cell Lines
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All human T-ALL cell lines (KOPT-K1, DND41, JURKAT, SUP-T13, HPB-ALL, MOLT-4, MOLT-16, RPMI-8402, TALL-1, and LOUCY) were obtained from ATCC (Manassas, VA, USA) or DSMZ (Braunschweig, Germany). The creation of Ba/F3 cells transformed by TYK2-E957D was described previously.6 (link) JURKAT and KOPT-K1 cells overexpressing BCL2 were generated with the MSCV-IRES-GFP retroviral expression system. JURKAT and KOPT-K1 cells overexpressing BCLXL or MCL1 cDNA were generated with the pHAGE-CMV-IRES-ZsGreen lentiviral expression system. For additional information, see Supplementary Materials and Methods . These cells were maintained in RPMI-1640 medium (GIBCO, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA).
3
Human T-ALL Cell Line Culturing
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In the present study, human T-ALL cell lines, including TALL-1, KOPTK1, Jurkat, CCRF-CEM and Molt16, were purchased from the American Type Culture Collection (ATCC). The cells mentioned above were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin, (NanJing SunShine Biotechnology Co., Ltd.) and 100 µg/ml streptomycin (Sunshine Biotechnology, Nanjing, China). The cells were maintained in a humidified atmosphere with 5% CO2 at 37˚C.
Clinical samples were obtained from Zibo Central Hospital (Zibo, China) from February 2018 to April 2019. Blood samples were obtained from 20 pediatric patients (age range, 3.6-14 years; 10 males, 10 females) who were diagnosed with T-ALL and 20 healthy donors (age range, 3-15.2 years; 10 males, 10 females). All fresh blood samples were immediately separated into several portions, snap-frozen in liquid nitrogen and stored at -80˚C prior to protein and RNA extraction. The present study was approved by the Ethical Review Committee of Zibo Central Hospital. All participants and their legal guardians agreed to the use of their samples in the present study and written informed consents were obtained from all of the legal guardians of all participants.
Clinical samples were obtained from Zibo Central Hospital (Zibo, China) from February 2018 to April 2019. Blood samples were obtained from 20 pediatric patients (age range, 3.6-14 years; 10 males, 10 females) who were diagnosed with T-ALL and 20 healthy donors (age range, 3-15.2 years; 10 males, 10 females). All fresh blood samples were immediately separated into several portions, snap-frozen in liquid nitrogen and stored at -80˚C prior to protein and RNA extraction. The present study was approved by the Ethical Review Committee of Zibo Central Hospital. All participants and their legal guardians agreed to the use of their samples in the present study and written informed consents were obtained from all of the legal guardians of all participants.
4
Cultivation of T-ALL Cell Lines
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Human T-ALL cell lines (HPB-ALL, TALL-1, ALL-SIL, CUTLL1) and peripheral blood mononuclear cells (PBMC) as control were both procured from the American Type Culture Collection (ATCC; Rockville, MD), cultivated in RPMI-1640 medium (Gibco, Grand Island, NY) at 37°C in 95% air/5% CO2. Approximately 10% fetal bovine blood (FBS; Gibco) was employed for cell culture.
5
Culturing Human T-ALL Cell Lines
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Human T-ALL cell lines (HPB-ALL, TALL-1, KOPTK1, Jurkat, CCRF-CEM, and Molt 4) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Molt 4-luciferase (Molt 4-luc) cells with stable luciferase expression were obtained from PerkinElmer (Santa Clara, CA, USA). T-ALL cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640; Gibco, BRL, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco). All cells were incubated in a humidified incubator with 5% CO2 at 37°C. Cells from passages 2–4 were used for experiments.
6
CD38+ Cell Line Binding Assay
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CD38+ cells including multiple myeloma cell lines (RPMI 8226, NCI-H929, MM.1S), Burkitt’s lymphoma cell lines (Ramos, Daudi, Raji), diffuse large B lymphoma cell line (Toledo), acute B lymphoid leukemia cell lines (RS4;11, NALM-6), and acute T lymphoid leukemia cell lines (CCRF-CEM, TALL-1, HuT 78) were obtained from American Type Culture Collection. Cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 2 mM L-Glutamine (HyClone) at 37°C in a humidified 5% CO2 incubator. 5×104 tumor cells were incubated with serial dilutions of CM313 or isotype control for 45 min. After washing and incubation with Alexa Fluor 647-conjugated goat anti-human IgG (Jackson ImmunoResearch) for a further 45 min, the cells were resuspended in propidium iodide (PI). Cell-associated fluorescence was analyzed using a FACSCelesta with FACSDiva™ software (BD Biosciences).
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