The pharmacological agents were administered at the following concentrations in vivo: recombinant human IL-1β (Prospec-Tany Techno Gene), 0.3, 1, 3, and 10 ng in 1 μl, i.c.v.51 (link)52 (link); recombinant human IL1Ra (IL1R antagonist, Prospec-Tany Techno Gene), 2, 20, 50, and 100 ng in 1 μl, i.c.v.52 (link); SR 141716 A (CB1R antagonist, Cayman Chemical), 1 and 10 mg/kg, i.p.53 (link); WIN 55,212-2 (CB1R agonist, Cayman Chemical), 0.5 mg/kg, i.p.54 (link); RHC 80267 (inhibitor of diacylglycerol lipase, Sigma), 1 mg in 2 μl, i.c.v.55 (link); KA (Sigma), 0.5 μg/μl, i.c.v. The solvent of SR 141716 A and WIN 55,212-2 was 80% saline +10% ethanol +10% Tween 20.
Recombinant human il 1β
Manufactured by ProSpec
Sourced in Israel
Recombinant human IL-1β is a cytokine protein produced using recombinant DNA technology. It is a key mediator of the inflammatory response.
Automatically generated - may contain errors
Lab products found in correlation
Rhc80267, by Merck Group (1 mentions)
Sr141716a, by Cayman Chemical (1 mentions)
Win55 212 2, by Cayman Chemical (1 mentions)
Recombinant human il 1ra, by ProSpec (1 mentions)
Mk2206, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Clozapine, by Merck Group (1 mentions)
Cell culture medium, by Sartorius (1 mentions)
Tnf α, by ProSpec (1 mentions)
Ifn γ, by ProSpec (1 mentions)
2 protocols using recombinant human il 1β
1
In Vivo Administration of Pharmacological Agents
2
Cytokine-induced cell culture assay
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The cell culture medium and the supplements were obtained from Biological Industries (Beit HaEmek, Israel). The recombinant human IL-1β, IFNγ, and TNFα were provided by ProSpec-Tany TechnoGene Ltd. (Rehovot, Israel).
Clozapine was purchased from Sigma Chemical (St. Louis, MO, USA) and MK-2206 from Sigma-Aldrich (St. Louis, MO, USA). Stock solutions of Clozapine (10 mM) and MK-2206 (29 mM) were prepared using dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA) and diluted in culture medium to final concentration of 10 µM and 1 µM, respectively. DMSO concentration in the cell cultures was ≤0.1% in all experiments.
Clozapine was purchased from Sigma Chemical (St. Louis, MO, USA) and MK-2206 from Sigma-Aldrich (St. Louis, MO, USA). Stock solutions of Clozapine (10 mM) and MK-2206 (29 mM) were prepared using dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA) and diluted in culture medium to final concentration of 10 µM and 1 µM, respectively. DMSO concentration in the cell cultures was ≤0.1% in all experiments.
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