K pneumoniae atcc baa 1705

K. pneumoniae ATCC BAA-1705 carrying bla_KPC-2, and K. pneumoniae ATCC BAA-2472 carrying bla_NDM-1 were obtained from American Type Culture Collection (ATCC). Both strains harboring carbapenem resistance genes were confirmed using PCR with specific primers as previously described [48 (link)]. The MICs of meropenem alone or in combination with tBLIP-II, or tBLIP-II-CPP against K. pneumoniae ATCC BAA-1705 and K. pneumoniae ATCC BAA-2472 were determined using the broth microdilution method followed CLSI recommendations [49 ]. Briefly, bacterial suspensions from freshly grown mid-log phase cultures were diluted to a concentration of 10⁶ CFU/mL in cation-adjusted Mueller-Hinton broth (CAMHB), and added to each well containing various concentrations of meropenem along with the recombinant proteins. The final concentrations of meropenem ranged from 256 to 1 μg/mL, with fixed concentrations of 10 μM and 20 μM for tBLIP-II, or tBLIP-II-CPP. The suspensions were then incubated for 20–24 h at 37°C. The MIC was determined as the lowest concentration of meropenem alone or combined agents at which there was a complete absence of bacterial growth.