Rotary evaporator re301

Freshly collected matured seeds of G. lotoides were thoroughly washed with tap water to eliminate any contaminants. After complete drying of the sample sunshade, it was pulverized by an electrical grinder. The dry powdered seeds of G. lotoides were subjected to extraction employing. One kg sample was soaked in a total of 5.7 litter of 80% hydromethanol with continuous shaking employing automatic shaker (Stuart Scientific) at 120 rpm at room temperature for 72 h. Then, the solution was primarily filtered employing muslin cloth and then refiltered with Whatman filters paper No.1. The marc was re-soaked with the same volume of solvents twice. Then subsequent filtrates were dried in to solid mass in a rotary evaporator (YAMATO rotary evaporator RE301, Japan, 40 °C, 60 rpm). The concentrated filtrate was subjected to deep freezer (DN-86W258) and then freeze-dried in a lyophilizer (Labfreeze Instruments Group Co., Ltd., Japan) at −50 °C. Ultimately, the %yield of extract was computed and kept at −4 °C using sealed container for experimentation [26 (link)].
The percentage yield of the rude extract $\text{\%Yield}=\frac{\text{ Seed extract weight obtained}(\text{g})}{\text{ Seed powder employed as stock}}\times 100$