Cartilage constructs were custom fabricated based on previously described methods.10 (link),12 (link),17 (link) Briefly, porcine ASCs were suspended in type I oligomeric collagen and polymerized. They were then compressed to constructs (412 mg total collagen, 3.4 cm in diameter, 5 mm in thickness) and cultured in chondrogenic differentiation medium (Hyclone Advance Stem Chondrogenic Differentiation Medium, SH30889.02; Thermo Scientific, Waltham, MA) and were maintained in a 37°C, 5% CO2 incubator for 2 weeks. The medium was changed twice per week.
Hyclone advance stem chondrogenic differentiation medium
Manufactured by Thermo Fisher Scientific
Hyclone Advance Stem Chondrogenic Differentiation Medium is a laboratory reagent designed to facilitate the differentiation of stem cells into chondrocytes, the primary cell type found in cartilage tissue. This medium provides the necessary growth factors and supplements to support the chondrogenic differentiation process.
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Lab products found in correlation
2 protocols using hyclone advance stem chondrogenic differentiation medium
1
Fabrication of Porcine ASC Cartilage Constructs
2
Fabrication of Cartilage Constructs
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