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Lipase

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Lipase is an enzyme that catalyzes the hydrolysis of lipids, such as triglycerides, into fatty acids and glycerol. It is a commonly used laboratory reagent for the analysis and manipulation of lipid-based compounds.

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Lab products found in correlation

Bile salt, by Yuanye Bio-Technology (2 mentions) Dextran standard, by Merck Group (1 mentions) Tripsin, by Merck Group (1 mentions) Xylose, by Merck Group (1 mentions) Mannose, by Merck Group (1 mentions) Arabinose, by Merck Group (1 mentions) Rhamnose, by Merck Group (1 mentions) Fucose, by Merck Group (1 mentions) Galactose, by Merck Group (1 mentions) Pepsin, by Merck Group (1 mentions) Pancreatin, by Merck Group (1 mentions) Xanthan gum, by Maclin Biochemical Technology (1 mentions) Sodium caseinate, by Maclin Biochemical Technology (1 mentions) Nile blue a, by Merck Group (1 mentions) Nile red, by Merck Group (1 mentions) Whey protein, by Yuanye Bio-Technology (1 mentions) α amylase, by Yuanye Bio-Technology (1 mentions) Pancreatin, by Yuanye Bio-Technology (1 mentions) Soybean lecithin, by Tokyo Chemical Industry (1 mentions) Sodium hydroxide (naoh), by Sinopharm (1 mentions)

5 protocols using lipase

1

Extraction and Characterization of Hericium erinaceus Polysaccharides

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The fruiting body of HE was purchased from Jilin Jiaohe Songshan Food Co., Ltd. (Jiaohe, China). Dextran standards (670, 410, 270, 80, 25, 12, and 5k Da) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Monosaccharide standards, including arabinose, mannose, fucose, xylose, rhamnose, galactose, and glucose were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Pancreatin, pepsin, and tripsin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Bile salt and lipase were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). 3,5-Dinitrosalicylic acid (DNS) and Fructo-oligosaccharide (FOS) were purchased from Huacheng Biological Technology Co., Ltd. (Changchun, China). All solvents used for the HPLC analysis were of HPLC grade. Other chemical reagents were of analytical grade.
Yang Y., Zhao C., Diao M., Zhong S., Sun M., Sun B., Ye H, & Zhang T. (2018). The Prebiotic Activity of Simulated Gastric and Intestinal Digesta of Polysaccharides from the Hericium erinaceus. Molecules, 23(12), 3158.
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2

Characterization of Biopolymer-Based Emulsions

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CaCO3 was obtained from Shuang Teng Industrial Co., Ltd. (Henan, China). Sodium caseinate (Lot#C10185129) and Xanthan gum were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Soybean Oil was supplied from COFCO Co., Ltd. (Tianjin, China). Nile Blue A and Nile Red were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The stomach pepsin (Lot#L22M9J56574, the activity was 1:10,000) from porcine gastric mucosa, lipase (Lot#T17N9C75063, BR, the activity was 30,000 U/g) from porcine pancreas and bile salt (Lot#K01N8B47111, cholic acid content was ≥60%) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals were analytical grade purchased from Sinopharm Group Co., Ltd. (Beijing, China).
Zhang J., Li G., Xu D, & Cao Y. (2022). Enhancing the Dispersion Stability and Sustained Release of S/O/W Emulsions by Encapsulation of CaCO3 Droplets in Sodium Caseinate/Xanthan Gum Microparticles. Foods, 11(18), 2854.
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3

Enzymatic Processing of Jackfruit

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Fully ripe jackfruits (Malaysia No.1) were purchased from a local supermarket, which harvested in June 2023 from Hainan, China. Whey protein (purity: 98 %), α-amylase (≥12 U/mg), lipase (≥30,000 U/g) were purchased from Yuanye-Bio Technology (Shanghai, China). Culture Medium was purchased from Solarbio Science (Beijing, China). All the different reagents used in this study are of analytical grade.
Suo H., Xiao S., Wang B., Cai Y.X, & Wang J.H. (2024). Waste to Wealth: Dynamics and metabolic profiles of the conversion of jackfruit flake into value-added products by different fermentation methods. Food Chemistry: X, 21, 101164.
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4

Extraction and Characterization of Monk Fruit Mogrosides

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The MV in this study was provided
by Guilin Sanling Biological Technology Co., Ltd. (Guangxi Province,
China) and was extracted and purified from S. grosvenorii (the purity of MV was further identified in the next section). The
MV standard was purchased from DeSiTe Biological Technology Co. Ltd.
(Chengdu, China). Fructooligosaccharide was purchased from Qiyun Biotechnology
Co., Ltd. (Guangzhou, China). Pepsin and trypsin were purchased from
Sigma Chemical Co., Ltd. (St. Louis, MO). Pancreatin and lipase were
obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). [2,2-Azinobis-(3-ethyl-benzothiazoline-6-sulfonic
acid)] diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH),
phenazine methosulfate (PMS), nicotinamide adenine dinucleotide (NADH),
nitroblue tetrazolium (NBT), and salicylic acid were purchased from
Newprobe Biotechnology Co., Ltd. (Beijing, China). Standards of SCFAs
(acetic, propionic, butyric, isobutyric, valeric, and isovaleric acids)
and all other chemicals were obtained from Aladdin Industrial Inc.
(Shanghai, China).
Xiao R., Liao W., Luo G., Qin Z., Han S, & Lin Y. (2021). Modulation of Gut Microbiota Composition and Short-Chain Fatty Acid Synthesis by Mogroside V in an In Vitro Incubation System. ACS Omega, 6(39), 25486-25496.
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5

NaCh Hydrate-based Lipid Formulation

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NaCh hydrate was obtained from Beijing Budweiser Technology CO., Ltd. Soybean lecithin was obtained from Tokyo Chemical Industry CO., Ltd. The molecular structures of NaCh and Soybean lecithin are shown in Scheme 1. Medium-chain triacylglycerols (MCT) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Tris (hydroxymethyl) methyl aminomethane was obtained from Shanghai Macklin Bio-Technology Co., Ltd. Hydrochloric acid (HCl) and NaOH were obtained from Sinopharm Chemical Reagent Co., Ltd. Sodium chloride (NaCl) and calcium chloride (CaCl 2 ) were obtained from Beijing J&K Scientific Ltd. Lipase was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Ultrapure water with a resistivity of 18.2 MΩ.cm was used for the preparations of solutions. n-Decane, with a purity of 99.5％, was obtained from Beijing Budweiser Technology CO., Ltd. All the other chemicals used in this study were of analytical grade.
Mekkaoui A., Liu Y., Zhang P., Ullah S., Wang C, & Xu B. (2021). Effect of Bile Salts on the Interfacial Dilational Rheology of Lecithin in the Lipid Digestion Process. Journal of oleo science, 70(8).
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