Myoglobin from human heart

Different concentrations of NaCl (Sigma, Steinheim, Germany), CaCl₂·2H₂O (Sigma, Neu-Ulm, Germany), FeCl₃·6H₂O (Merck, Darmstadt, Germany), disodium EDTA·2H₂O (Sigma, Steinheim, Germany), trisodium citrate·2H₂O (Sigma, Vienna, Austria), sodium heparin (Novo, Vienna, Austria), sodium dodecyl sulfate (SDS, Merck, Darmstadt, Germany), proteinase K (Ambion, Kaufungen, Germany), urea (Sigma, Steinheim, Germany), bile salts (Sigma, Steinheim, Germany), hemoglobin and lactoferrin from human blood (Sigma, Steinheim, Germany), bovine IgG (Sigma, Steinheim, Germany), and myoglobin from human heart (Sigma, Steinheim, Germany) were prepared in DEPC treated water to investigate their effects on rTth polymerase to detect RNA. The final concentrations in the real-time RT-PCR mixtures were 10, 20, 40, 60, 80, 100 mM NaCl; 6, 8, 10, 12, 14, 16 mM CaCl₂; 0.01, 0.1, 0.5, 1, 5, 10 mM FeCl₃; 1, 2, 4, 6, 8, 10 mM EDTA; 1, 5, 10, 20, 40, 60 mM citrate; 1, 5, 10, 20, 40, 60 IU heparin; 10, 20, 50, 100, 150, 200 μg/ml SDS; 5, 10, 20, 40, 60, 80 μg/ml proteinase K; 50, 100, 150, 200, 300, 400 mM urea; 10, 50, 100, 200, 500, 1000 μg/ml bile salts; 5, 10, 20, 50, 100, 200 μg/ml lactoferrin; 0.1, 0.2, 0.5, 1, 2, 5 mg/mL hemoglobin; 10, 20, 50, 100, 200, 300 μg/ml IgG; 30, 60, 90, 120, 300, 600 μg/ml myoglobin.