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Goat anti mouse igg

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Goat anti-mouse IgG is an antibody reagent that binds to mouse immunoglobulin G (IgG) molecules. It is commonly used in immunoassays and other applications that require the detection or isolation of mouse IgG.

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Lab products found in correlation

Hrp conjugated anti v5 antibody, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti gfp antibody, by Roche (1 mentions) Peroxidase conjugated goat anti rabbit igg, by Dianova (1 mentions) Goat anti rabbit igg coupled to alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Rabbit anti lc3, by Abcepta (1 mentions) Rabbit anti mnsod, by Merck Group (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Tnf α, by R&D Systems (1 mentions) Irak1, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Il 1β, by R&D Systems (1 mentions) Goat anti rabbit igg, by Dianova (1 mentions) Anti bcl 2, by BD (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti hsp60, by BD (1 mentions) Anti mcl 1, by BD (1 mentions) Anti bim, by Merck Group (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions) Anti bip, by Cell Signaling Technology (1 mentions)

7 protocols using goat anti mouse igg

1

Immunoblotting antibodies for mitochondrial proteins

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Rabbit anti-human GCDH antibody was kindly provided by Dr. S. I. Goodman (University of Colorado Health Sciences Center, Denver). The polyclonal mouse anti-human DLST and rabbit anti-human ETFA antibodies were purchased from Sigma (Munich, Germany), rabbit anti-human ETFB from Abcam (Cambridge, UK), and rabbit anti-LC3 from Abgent (San Diego, USA). The monoclonal mouse anti-GFP antibody was obtained from Roche (Mannheim, Germany) and rabbit anti-MnSOD from Millipore (Billerica, USA). Peroxidase-conjugated goat anti-rabbit IgG and goat anti-mouse IgG was from Dianova (Hamburg, Germany). HRP-conjugated anti-V5 antibody, monkey anti-mouse IgG coupled to Alexa Fluor 488 and goat anti-rabbit IgG coupled to Alexa Fluor 546 were from Invitrogen (Karlsruhe, Germany).
Schmiesing J., Schlüter H., Ullrich K., Braulke T, & Mühlhausen C. (2014). Interaction of Glutaric Aciduria Type 1-Related glutaryl-CoA Dehydrogenase with Mitochondrial Matrix Proteins. PLoS ONE, 9(2), e87715.
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2

Analyzing IRAK1 and IκBα in Fibroblasts

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To analyze IRAK1 expression and IκBα degradation, we stimulated SV40-immortalized fibroblasts of P1, P2, P3, and P4 as well as of healthy controls and IRAK1-deficient and IRAK4-deficient controls with IL-1β (10 ng/ml, R&D Systems) and TNF-α (20 ng/ml, R&D Systems) for 15, 30, 45, 60, and 90 min as well as with IL-1β (10 ng/ml, R&D Systems), TLR4 agonist lipopolysaccharide (LPS) (10 µg/ml, Sigma-Aldrich), and TNF-α (20 ng/ml, R&D Systems) for 20, 60, 120, and 240 min, respectively. The further steps were performed as described previously using the following antibodies: IκBa (610,690, BD Biosciences), IRAK1 (sc-7883, Santa Cruz Biotechnology), IRAK-4 (ADI-KAP-ST206-E, Enzo), glycerinaldehyd-3-phosphat-dehydrogenase (GAPDH) (sc-25778, Santa Cruz Biotechnology), Goat Anti Rabbit IgG (111–035-045, Dianova), and Goat Anti Mouse IgG (115–035-062, Dianova) [118 (link)]. Detailed protocols are available upon request.
Gottschalk I., Kölsch U., Wagner D.L., Kath J., Martini S., Krüger R., Puel A., Casanova J.L., Jezela-Stanek A., Rossi R., Chehadeh S.E., Van Esch H, & von Bernuth H. (2022). IRAK1 Duplication in MECP2 Duplication Syndrome Does Not Increase Canonical NF-κB–Induced Inflammation. Journal of Clinical Immunology, 43(2), 421-439.
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3

Comprehensive Lysis and Immunoblotting Protocol

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Cells were lysed either in 8 M Urea or in RIPA buffer (Thermo Fisher, #89900) and boiled in Laemmli-buffer containing DTT. Samples were heated for 5 min. Antibodies used were: Bak(NT), Bak(aa23-38), Bak(Ab-1), Bak(G23) as above. The following antibodies were purchased from Cell Signaling unless indicated otherwise: anti-Bim (# C34C5), anti-GAPDH (Millipore, #MAB374), anti-Bcl-XL (#54H6), anti-Bcl-2 (#2870), anti-Bax (#2772), anti-VDAC (#4661), anti-Hsp60 (#4870), anti-Mcl-1 (BD, #559027), anti-Hsp60(Ctr) (Enzo Life Sciences, #ALX-804-072), anti-Tom22 (Santa Ccruz, sc-58308), anti-Bcl-w (#2724 S), anti-GFP (Roche, #11814460001), anti-cytochrome c (#11940) and anti-tBid [64 (link)], anti-BiP (Cell signalling, #3177), anti-VDAC2/3 (Thermo Fisher, #PA141205). Peroxidase-conjugated secondary antibodies were goat anti-rabbit IgG (Sigma, #A6667), goat anti-rabbit Fc (Sigma, #AP156P), goat anti-mouse IgG (Dianova, #115035166), goat anti-mouse Fc (Sigma, #AP127P) and goat anti-rat IgG (Dianova, #112035062).
Waguia Kontchou C., Gentle I.E., Weber A., Schoeniger A., Edlich F, & Häcker G. (2022). Chlamydia trachomatis inhibits apoptosis in infected cells by targeting the pro-apoptotic proteins Bax and Bak. Cell Death and Differentiation, 29(10), 2046-2059.
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4

Western Blot Antibody Conditions

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The following antibodies and dilutions were used for Western blotting: mouse anti-E-cadherin (BD Pharmingen, 610181; 1:5,000), mouse anti-β-Actin (Sigma, A5441; 1:5,000), mouse anti-CD44 (R&D Systems, BBA10; 1:1000), mouse anti-HAS2 (Abcam, H00054845-B01P; 1:500) and rabbit anti-ZEB1 (Sigma, HPA027524; 1:5,000), as well as HRP-coupled goat anti-rabbit IgG (Dianova, 111-035-003; 1:25,0000) and goat anti-mouse IgG (Dianova, 115-035-003; 1:25,000).
Preca B.T., Bajdak K., Mock K., Lehmann W., Sundararajan V., Bronsert P., Matzge-Ogi A., Orian-Rousseau V., Brabletz S., Brabletz T., Maurer J, & Stemmler M.P. (2017). A novel ZEB1/HAS2 positive feedback loop promotes EMT in breast cancer. Oncotarget, 8(7), 11530-11543.
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5

MAIGA Protocol for IgG Quantification

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The original MAIGA protocol 19 was modified. In brief, 3 × 10 5 paraformaldehyde-fixed 293F cells were sensitized with 50 μL serum, washed, incubated with 10 μL capture moab (20 μg/mL), and lysed afterwards in 100 μL lysis buffer (2.4 g/L Tris, 8.76 g/L NaCl, 9.5 mL/L Triton X-100, and 1.86 g/L EDTA; pH 7.4) Cell lysates were immobilized onto a microtiter plate precoated with goat anti-mouse IgG. Bound human IgG was then detected with horseradish peroxidase-labeled goat anti-human IgG (Dianova), followed by incubation with the enzyme substrate (o-phenylenediamine) and colorimetric determination in an absorbance reader (Tecan) at 492 nm. The cut-off was defined as the twofold absorbance obtained with mock-transfected 293F cells.
Sachs U.J., Radke C., Bein G., Grabowski C., Simtong P., Bux J., Bayat B, & Reil A. (2020). Primary structure of human neutrophil antigens 1a and 1b. Transfusion, 60(4).
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6

TLC-based Detection of Glycolipid Antibodies

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Polyclonal chicken IgY anti-Gb3Cer and anti-Gb4Cer antibodies with previously described specificities [42 (link),74 (link),75 (link)] and a monoclonal rat IgM anti-Forssman GSL antibody (clone IIC2), produced by Bethke and co-workers [41 (link)] and described as a highly specific tool for the detection of Forssman GSL [25 (link),38 (link),76 (link)], were employed for TLC overlay assays. Stx2e-containing supernatant of STEC strain S123G of serotype O139:K82 was used as previously described [25 (link)]. Mouse anti-Stx2 antibody (clone VT 135/6-B9, 2.75 mg/mL) was purchased from SIFIN GmbH (Berlin, Germany). Secondary alkaline phosphatase (AP)-conjugated affinity-purified polyclonal rabbit anti-chicken IgY (code 303-055-033), goat anti-rat IgG + IgM (code 112-055-044) and goat anti-mouse IgG (code 115-055-003) antibodies were from Dianova (Hamburg, Germany).
Steil D., Bonse R., Meisen I., Pohlentz G., Vallejo G., Karch H, & Müthing J. (2016). A Topographical Atlas of Shiga Toxin 2e Receptor Distribution in the Tissues of Weaned Piglets. Toxins, 8(12), 357.
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7

Immunoblotting for HIV-1 Proteins

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Immunoblotting for HIV-1 Gag/CA p24 was described previously (63 (link)). Immunoblotting for Rev was performed with primary antibody sheep anti-Rev (1:4,500, no. H6006; US Biological Life Sciences) and secondary antibody rabbit anti-goat Ig/HRP (1:5,000, no. P0160; Dako). Immunoblotting for myc-tagged proteins and for GFP was performed using primary antibodies monoclonal mouse anti-myc tag (1:8,000, no. 2276; Cell Signaling Technology) and monoclonal mouse anti-GFP (1:500, no. sc-9996; Santa Cruz) in combination with the secondary antibody goat anti-mouse IgG (1:5,000, no. 115-035-146; Dianova). Immunoblotting for α-tubulin was performed using primary antibody polyclonal rabbit anti-α-tubulin antiserum (1:4,000, no. 600-401-880; Rockland), and secondary swine anti-rabbit Ig/horseradish peroxidase (HRP) (1:5,000, no. P0217; Dako). In experiments shown in Fig. 1B, 6A, and 6C, anti-α-tubulin blotting was conducted after stripping the blotted anti-CA and anti-myc membranes with stripping buffer (no. 2504; Millipore). The membranes were then reblocked and subsequently reblotted with antibodies as described above. Immunoblotting for LaminB was performed with primary antibody goat anti-LaminB (C-20) (1:500, no. sc-6216; Santa Cruz Biotechnology) and secondary antibody rabbit anti-goat Ig/HRP (1:5,000, no. P0160; Dako).
Xiao H., Wyler E., Milek M., Grewe B., Kirchner P., Ekici A., Silva A.B., Jungnickl D., Full F., Thomas M., Landthaler M., Ensser A, & Überla K. (2021). CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and Cellular mRNAs. mBio, 12(1), e02525-20.
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