Rag2 γc

Adult Albino Oxford strain rats were produced and maintained in the Baker Institute vivarium. ΔdblGATA (eosinophil-ablated), PHIL (eosinophil-ablated), VertX (IL-10 reporter), Rag1^−/−, IL-5-expressing transgenic (NJ.1638) (IL-5Tg⁺), IL-5Tg⁺ × MHCII^−/−, IL-5Tg⁺ × IL-4^−/− mice were bred at Cornell Transgenic Mouse Core Facility and offspring were transferred to the Baker Institute. IL-5Tg⁺ × IL-4^−/− and IL-5Tg⁺ × MHCII^−/− mice were generated by crossing and backcrossing on the deficient strains and genotype was confirmed by PCR. IL-10^−/− mice were purchased from The Jackson laboratory. Rag2^−/−γc^−/− (innate lymphoid cell-ablated) mice were purchased from Taconic. Arg1^flox/flox;Tie2cre (Arginase1 specifically ablated in myeloid cells) mice were a gift from Dr. Thomas Wynn (NIAID). PHIL mice were genotyped as described previously (23 (link)). All strains were on a C57BL/6 background. C57BL/6^NHsd mice were purchased from Taconic as wild type (WT) control. Animal care was in accordance with the guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care and experiments were performed with the approval of the Institutional Animal Care and Use Committee of Cornell University.

Adult C57BL/6 WT, Rag2^−/−, Rag2^−/−γc^−/−, CD1d^−/− mice were purchased from Taconic. All mice were housed in a specific pathogen free room; all Listeria-infected mice were housed in specific ABSL-2 facility. For i.v. infections, mice were anesthetized with Ketamine 80 mg/kg and Xylazine 10 mg/kg (i.p., in 200 μl PBS). Intravenous infections were performed retro-orbitally. Blood and tissue samples were collected and processed at the indicated time points in accordance with University of Michigan Animal Care and Use Committee and approval to use mice was granted by the University of Michigan in accordance with the US National Institute of Health requirements for the care and use of animals. Care for mice was provided in accordance with PHS and AAALAC standards.