The colloidal stability of DB-HNTs in aqueous solution was studied by dynamic light scattering (Malvern Zeta Size Nano S-90 DLS instrument). The refractive indexes of gold and platinum were adapted from the Malvern Reference manual (MAN0396 Issue 1.0). The refractive index of 0.30 was adapted for gold, while the refractive index of platinum was 4.5. The stability of functionalized DB-HNTs was validated by measuring the hydrodynamic size distribution of functionalized DB-HNTs in serum using DLS. The DB-HNTs were added to mouse serum in phosphate buffered saline (PBS) at a concentration of 4 nM and then incubated at 37 °C for 24 h. The aliquots were analyzed at 0, 4, 8, 24, and 48 h post-incubation by DLS. Each aliquot was filtered using a spin filter (MWCO = 30 kDa), and further washed with water 3 times. The eluent was collected and analyzed by ICP-MS for trace amount of Fe, Pt, Au and Gd.
Zeta size nano s 90 dls instrument
Manufactured by Malvern Panalytical
The Zeta Size Nano S-90 DLS instrument is a dynamic light scattering (DLS) system used for particle size and zeta potential analysis. It measures the size and surface charge of particles and molecules in the nanometer to micron range. The instrument utilizes a laser light source and a detector to analyze the Brownian motion of the particles, providing information about their hydrodynamic size and zeta potential.
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2 protocols using zeta size nano s 90 dls instrument
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Evaluating DB-HNTs Colloidal Stability
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Evaluating DB-HNTs Colloidal Stability
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The colloidal stability of DB-HNTs in aqueous solution was studied by dynamic light scattering (Malvern Zeta Size Nano S-90 DLS instrument). The refractive indexes of gold and platinum were adapted from the Malvern Reference manual (MAN0396 Issue 1.0). The refractive index of 0.30 was adapted for gold, while the refractive index of platinum was 4.5. The stability of functionalized DB-HNTs was validated by measuring the hydrodynamic size distribution of functionalized DB-HNTs in serum using DLS. The DB-HNTs were added to mouse serum in phosphate buffered saline (PBS) at a concentration of 4 nM and then incubated at 37 °C for 24 h. The aliquots were analyzed at 0, 4, 8, 24, and 48 h post-incubation by DLS. Each aliquot was filtered using a spin filter (MWCO = 30 kDa), and further washed with water 3 times. The eluent was collected and analyzed by ICP-MS for trace amount of Fe, Pt, Au and Gd.
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