6500 q trap

The flavonoid metabolomics of samples were analyzed as described previously [3 (link)], using a UPLC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Q TRAP, Foster City, USA) equipped with a C18 chromatographic column (Waters ACQUITY UPLC HSS T3, 2.1 mm × 100 mm, 1.8 µm). Phase A of the mobile phase (0.04% acetic acid in water) and phase B of the mobile phase (0.04% acetic acid in acetonitrile) were used as solvent systems. The following was the gradient program (A:B): 95:5 (v/v) at 0 min, 5:95 (v/v) at 11.0 min, 5:95 (v/v) at 12.0 min, 95:5 (v/v) at 12.1 min, 95:5 (v/v) at 15.0 min, and 95:5 (v/v) at 15.0 min; flow rate 0.40 mL/min. The injection volume was 2.0 µL, and the column temperature was held at 40 °C. A Q TRAP-MS was used to analyze the effluent.

The extracted samples were analyzed as described by Wang et al. [28 (link)]. A UPLC-ESI-MS/MS system (UPLC, Shim-pack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Q TRAP, Foster City, USA) equipped with a C18 chromatographic column (Waters ACQUITY UPLC HSS T3, 2.1 mm × 100 mm, 1.8 μm) was used for the analysis. The solvent systems contained mobile phase A (0.04% acetic acid in water) and mobile phase B (0.04% acetic acid in acetonitrile). The gradient program (Mobile phase A: Mobile phase B) was performed as follows: 95:5 (v/v) at 0 min, 5:95 (v/v) at 11.0 min, 5:95 (v/v) at 12.0 min, 95:5 (v/v) at 12.1 min, and 95:5 (v/v) at 15.0 min; flow rate 0.40 mL/min. The column temperature was kept at 40 °C, and the injection volume was set to 2 μL. The UPLC effluent was input into an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS and analyzed further.