Log In Sign up
The largest database of trusted experimental protocols

Anti ehd3

Manufactured by Abnova
Visit Supplier

Anti-EHD3 is a laboratory reagent used for the detection and quantification of EHD3 protein in biological samples. EHD3 is a member of the EHD family of proteins involved in membrane trafficking and endocytosis. The Anti-EHD3 reagent can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of EHD3 in cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst, by Thermo Fisher Scientific (2 mentions) Anti ha hrp, by Roche (2 mentions) Anti rpgrip1l, by Proteintech (2 mentions) Anti ift20, by Proteintech (2 mentions) Mouse anti γ tubulin clone gtu 88, by Merck Group (2 mentions) Actub clone 6 11b 1, by Merck Group (2 mentions) Anti rhodopsin clone 4d2, by Merck Group (2 mentions) Anti β tubulin clone 2 28 33, by Merck Group (2 mentions) Rabbit anti pericentrin, by Novus Biologicals (2 mentions) Anti ehd2, by Abcam (2 mentions) Anti ehd4, by Abcam (2 mentions) Anti cep290, by Fortis Life Sciences (2 mentions) Anti tmem67, by Proteintech (2 mentions) Goat anti cep164, by Santa Cruz Biotechnology (2 mentions) Anti β actin clone ac 15, by Merck Group (2 mentions) Anti gfp, by Abcam (2 mentions)

2 protocols using anti ehd3

1

Antibody Panel for Cytoskeletal and Ciliary Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
+ Open protocol
+ Expand
2

Antibody Panel for Cytoskeletal and Ciliary Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial antibodies used were mouse anti-γ-tubulin (clone GTU-88, 1/5000, Sigma), anti-acetylated α-tubulin (Actub) (clone 6-11B-1, 1/10000, Sigma), anti-rhodopsin (clone 4D2, 1/100), anti-β–actin (clone AC-15, 1/30000, Sigma) and anti-β-tubulin (clone 2-28-33, 1/2000, Sigma), anti-EHD3 (1/1000, Abnova for IF and 1/100, Abcam, 1/500, Proteintech for WB), rabbit anti-pericentrin (1/5000, Novus Biologicals, 1/5000), anti-EHD2 (1/1000, Abcam), anti-EHD4 (1/1000, Abcam), anti-GFP (1/50, Abcam), anti-HA HRP (1:1000, Roche), anti-CEP290 (1/200, Bethyl), anti-RPGRIP1L (1/200, Proteintech), anti-IFT20 (1/200, Proteintech), anti-TMEM67 (1/200, Proteintech), anti-SNAP29 (1/1000 for WB Proteintech and 1/200 for IF from Dr. Andrew Peden) and goat anti-CEP164 (1/500, Santa Cruz). Rabbit anti-Rab11a (1/300) was a gift from Dr. Jim Goldenring and rabbit anti-CP110 (1/500) was a gift from Dr. Monica Diaz. Polyclonal rabbit anti-EHD1 (1/1000) has been previously described33 (link). Fluorescent secondary antibodies and phalloidin (1/50) were from Life Technologies (Molecular probes, Eugene, OR). Nuclei were labeled with Hoechst (Molecular probes, Eugene, OR).
Lu Q., Insinna C., Ott C., Stauffer J., Pintado P.A., Rahajeng J., Baxa U., Walia V., Cuenca A., Hwang Y.S., Daar I.O., Lopes S., Lippincott-Schwartz J., Jackson P.K., Caplan S, & Westlake C.J. (2015). Early steps in primary cilium assembly require EHD1- and EHD3-dependent ciliary vesicle formation. Nature cell biology, 17(3), 228-240.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!