Antibodies were obtained from several sources: anti-O-GlcNAc (RL-2, #MA1-072) from Pierce Biotechnology (Rockford, IL); anti-MAN1A1 (#M-3694) from Sigma-Aldrich (St. Louis, MO); anti-pAkt (ser-473, #9271s), anti-Akt, anti-pFOXO3 (ser-294, #5538s), and anti-pFOXO3 (thr-32, #9464s) from Cell Signaling (Danvers, MA); anti-Erk (K-23, #sc-94), anti-pErk (E-4, #sc-7383), anti-pIkk (T23, #sc-101706), and anti-Ikk (H470, #sc-7607) from Santa Cruz Biotechnology; anti-FOXO3 (#07-702) and anti-GAPDH (#MAB-374) from Merck Millipore (Billerica, MA). Akt inhibitor, MK-2206 dihydrochloride (#sc-364537) was from Santa Cruz Biotechnology (Santa Cruz, CA) and Erk inhibitor, PD98059 (#9900) was obtained from Cell Signaling (Danvers, MA). PNGase-F (#P0704) was purchased from New England Biolabs (Ipswich, MA), Pisum Sativum Agglutinin (PSA, #L-1050), biotinylated PSA (#B-1055), biotinylated Concanavalin A (#B-1005) were obtained from Vector Laboratories (Burlingame, CA). Mannosidase I inhibitor, kifunensine (#K1140), OGA inhibitor, [O-(2-Acetamido-2-deoxy-D-glucopyranosylidenamino)-N-phenylcarbamate; PUGNAc, #A7229], and cycloheximide (CHX, #C104450) were purchased from Sigma-Aldrich.
Anti pfoxo3 thr 32
Manufactured by Cell Signaling Technology
Anti-pFOXO3 (thr-32) is a lab equipment product that detects phosphorylated FOXO3 at threonine 32. This product is used to study the role of FOXO3 phosphorylation in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by Merck Group (2 mentions)
Anti gapdh mab374, by Merck Group (2 mentions)
Kifunensine, by Merck Group (2 mentions)
Pugnac, by Merck Group (2 mentions)
Pd98059, by Cell Signaling Technology (2 mentions)
Anti man1a1 m, by Merck Group (2 mentions)
Pngase f, by New England Biolabs (2 mentions)
Anti foxo3, by Merck Group (1 mentions)
2 protocols using anti pfoxo3 thr 32
1
Antibody Sources and Inhibitors for O-GlcNAcylation Analysis
2
Antibodies and Inhibitors for O-GlcNAc Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies were obtained from several sources: anti-O-GlcNAc (RL-2, #MA1-072) from Pierce Biotechnology (Rockford, IL); anti-MAN1A1 (#M-3694) from Sigma-Aldrich (St. Louis, MO); anti-pAkt (ser-473, #9271s), anti-Akt, anti-pFOXO3 (ser-294, #5538s), and anti-pFOXO3 (thr-32, #9464s) from Cell Signaling (Danvers, MA); anti-Erk (K-23, #sc-94), anti-pErk (E-4, #sc-7383), anti-pIkk (T23, #sc-101706), and anti-Ikk (H470, #sc-7607) from Santa Cruz Biotechnology; anti-FOXO3 (#07–702) and anti-GAPDH (#MAB-374) from Merck Millipore (Billerica, MA). Akt inhibitor, MK-2206 dihydrochloride (#sc-364537) was from Santa Cruz Biotechnology (Santa Cruz, CA) and Erk inhibitor, PD98059 (#9900) was obtained from Cell Signaling (Danvers, MA). PNGase-F (#P0704) was purchased from New England Biolabs (Ipswich, MA), Pisum Sativum Agglutinin (PSA, #L-1050), biotinylated PSA (#B-1055), biotinylated Concanavalin A (#B-1005) were obtained from Vector Laboratories (Burlingame, CA). Mannosidase I inhibitor, kifunensine (#K1140), OGA inhibitor, [O-(2-Acetamido-2-deoxy-D-glucopyranosylidenamino)-N-phenylcarbamate; PUGNAc, #A7229], and cycloheximide (CHX, #C104450) were purchased from Sigma-Aldrich.
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