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Las x software v 3

Manufactured by Leica
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Sourced in Germany

LAS X Software v.3.0.2 is a comprehensive software solution for microscope image acquisition, processing, and analysis. It provides a user-friendly interface and supports a wide range of Leica microscope hardware.

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Lab products found in correlation

Tcs sp8 confocal 2p microscope, by Leica (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

LAS X software v LAS X v.3.5.1.18803 software Software LAS-X 3.3 LAS X software LAS X LAS software

6 protocols using las x software v 3

1

Lipid Visualization in Hepatocytes

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Lipid accumulation in hepatocytes grown in fatty-acid-supplemented medium was visualized using coherent anti-stokes Raman scattering (CARS) microscopy. Images were recorded every 24 hr by tuning the Stokes (1,064 nm) and pump (817 nm) beams to excite the Raman peak at 2,845 cm−1 (663 nm) corresponding to the CH2 stretch in lipid molecules. The microscopy system was configured to detect both forward- and epi-CARS signals. All images were averaged over 64 frames. Overlays of forward-(green) and epi-CARS (red) signals were generated using raw images (LAS X Software v.3.0.2, Leica Microsystems).
Krishnan S., Ding Y., Saedi N., Choi M., Sridharan G.V., Sherr D.H., Yarmush M.L., Alaniz R.C., Jayaraman A, & Lee K. (2018). Gut Microbiota-Derived Tryptophan Metabolites Modulate Inflammatory Response in Hepatocytes and Macrophages. Cell reports, 23(4), 1099-1111.
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2

Lipid Visualization in Hepatocytes

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Lipid accumulation in hepatocytes grown in fatty-acid-supplemented medium was visualized using coherent anti-stokes Raman scattering (CARS) microscopy. Images were recorded every 24 hr by tuning the Stokes (1,064 nm) and pump (817 nm) beams to excite the Raman peak at 2,845 cm−1 (663 nm) corresponding to the CH2 stretch in lipid molecules. The microscopy system was configured to detect both forward- and epi-CARS signals. All images were averaged over 64 frames. Overlays of forward-(green) and epi-CARS (red) signals were generated using raw images (LAS X Software v.3.0.2, Leica Microsystems).
Krishnan S., Ding Y., Saedi N., Choi M., Sridharan G.V., Sherr D.H., Yarmush M.L., Alaniz R.C., Jayaraman A, & Lee K. (2018). Gut Microbiota-Derived Tryptophan Metabolites Modulate Inflammatory Response in Hepatocytes and Macrophages. Cell reports, 23(4), 1099-1111.
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3

Whole-Kidney Imaging with Confocal Microscopy

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Single plane tile‐imaging of the entire C57BL/6 kidney sections was conducted with a Leica TCS SP8 confocal/2P microscope (Leica Microsystems Inc.) available at the ICBM imaging facility, using a Leica HC PL APO CS2 20×/0.75IMM objective lens adjusted to oil immersion. Confocal images (12bit) were acquired at 1024 × 1024 pixels, 400 Hz, 1 AU, bidirectional X scan, 1.2 zoom, line averaging of 2, and using a sequential laser illumination mode set up to three sequences: (1) Nuclei/DAPI (405 nm laser illumination, PMT1 adjusted to collect 415–485 nm emission), (2) MAP3K7/Alexa Fluor 488 (488 nm laser illumination, PMT2 adjusted to collect 500–500 nm emission), (3) ICs/Cyanin‐3 (552 nm laser illumination, PMT3 adjusted to collect 560–630 nm emission light). The hardware scanning set up was kept constant for all samples. Mosaic images were generated using automatic stitching in Leica LAS X software v.3.5.7.
Saxena V., Arregui S., Kamocka M.M., Hains D.S, & Schwaderer A. (2022). MAP3K7 is an innate immune regulatory gene with increased expression in human and murine kidney intercalated cells following uropathogenic Escherichia coli exposure. Journal of Cellular Biochemistry, 123(11), 1817-1826.
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4

Multicolor Kidney Tissue Imaging

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Single plane tile-imaging of the entire C57BL/6 kidney sections was conducted with a Leica TCS SP8 confocal/2P microscope (Leica Microsystems, Inc., Buffalo Grove, IL) available at the ICBM imaging facility (Indianapolis, IN), using a Leica HC PL APO CS2 20x/0.75IMM objective lens adjusted to oil immersion. Confocal images (12 bit) were acquired at 1024×1024 pixels, 400Hz, 1 AU, bidirectional X scan, 1.2 zoom, line averaging of 2, and using a sequential laser illumination mode set up to 3 sequences: 1) Nuclei/DAPI (405 nm laser illumination, PMT1 adjusted to collect 415–485 nm emission), 2) MAP3K7/Alexa Fluor 488 (488 nm laser illumination, PMT2 adjusted to collect 500–500nm emission), 3) ICs/Cyanin-3 (552 nm laser illumination, PMT3 adjusted to collect 560–630 nm emission light). The hardware scanning setup was kept constant for all samples. Mosaic images were generated using automatic stitching in Leica LAS X software v.3.5.7.
Saxena V., Arregui S., Kamocka M.M., Hains D.S, & Schwaderer A. (2022). MAP3K7 is an innate immune regulatory gene with increased expression in human and murine kidney intercalated cells following uropathogenic Escherichia coli exposure. Journal of Cellular Biochemistry, 123(11), 1817-1826.
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5

Immunofluorescence Imaging of Transfected A549 Cells

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A549 cells were seeded onto glass coverslips and cultured for 24 h before transfection with 1 μg of the indicated plasmids. Transfected A549 cells were cultured for 16 h, washed with PBS, and fixed with 4% paraformaldehyde. Cells were permeabilized with 0.25% (vol/vol) Triton-X in PBS, rinsed, and then blocked for 1 h in 1% (vol/vol) bovine serum albumin (BSA) in PBS. Coverslips were incubated with the indicated primary antibodies overnight at 4°C. Fluorescein (FITC)- and Alexa Fluor 594-labeled secondary antibodies were added for 1 h at room temperature before mounting onto glass slides using gold antifade mountant with DAPI (ThermoFisher Scientific catalog no. P26935). Slides were imaged with a Leica microscope coupled to Leica LAS X software v3.0.11.20652 (Leica Microsystems, Inc., Germany).
Chiok K., Pokharel S.M., Mohanty I., Miller L.G., Gao S.J., Haas A.L., Tran K.C., Teng M.N, & Bose S. (2022). Human Respiratory Syncytial Virus NS2 Protein Induces Autophagy by Modulating Beclin1 Protein Stabilization and ISGylation. mBio, 13(1), e03528-21.
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6

RSV Infection-Induced Autophagy Quantification

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A549 mCherry-LC3II cells were seeded onto glass coverslips and cultured for 24 h until subconfluent. Cells were infected with RSV for 16 h before the coverslips were fixed in 4% (vol/vol) paraformaldehyde. Fixed coverslips were washed in PBS and rinsed in double-distilled water before mounting onto glass slides using gold antifade mountant with DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific catalog no. P26935). Slides were imaged with a Leica microscope coupled to Leica LAS X software v3.0.11.20652 (Leica Microsystems, Inc., Germany). The number of LC3II puncta per cell was counted using the ImageJ particle analysis function (50 (link)). LC3II puncta were counted in at least 25 individual cells per experiment in two independent experiments.
Chiok K., Pokharel S.M., Mohanty I., Miller L.G., Gao S.J., Haas A.L., Tran K.C., Teng M.N, & Bose S. (2022). Human Respiratory Syncytial Virus NS2 Protein Induces Autophagy by Modulating Beclin1 Protein Stabilization and ISGylation. mBio, 13(1), e03528-21.
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