N n dimethylformamide (dmf)

Manufactured by AAPPTec

Sourced in United States

N,N dimethylformamide (DMF) is a colorless, polar, aprotic solvent commonly used in various laboratory applications. It has a high boiling point and is miscible with a wide range of organic solvents. DMF serves as a versatile reaction medium for organic synthesis, including peptide coupling reactions and other chemical transformations.

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Lab products found in correlation

Pgl3 control vector, by Promega (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Trifluoroacetic acid tfa, by Thermo Fisher Scientific (2 mentions) Acetic anhydride, by Merck Group (2 mentions) Gigaprep, by Qiagen (2 mentions) 1 2 ethanedithiol, by Merck Group (1 mentions) Fmoc amino acids, by AAPPTec (1 mentions) 4 methylpiperidine, by Merck Group (1 mentions) N n dicyclohexylcarbodiimide, by AAPPTec (1 mentions) Rink amide resin, by AAPPTec (1 mentions) Sephadex g 25, by Merck Group (1 mentions) Sodium 125iodine, by PerkinElmer (1 mentions) Piperidine, by Merck Group (1 mentions) Fmoc protected amino acids, by AAPPTec (1 mentions)

Spelling variants of this product

Dimethylformamide (2 citations)

3 protocols using n n dimethylformamide (dmf)

Synthetic Peptide Production Protocol

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The peptides were synthesized by manual SPPS using the manual Fmoc/tBu strategy. Briefly, Rink amide resin (AAPPTec, KY, USA) (0.46 meq/g) was used as a solid support. (a) The Fmoc group was removed by treating with 20% 4-methylpiperidine (Sigma-Aldrich, MO, USA) in N,N-dimethylformamide (AAPPTec, KY, USA). (b) For the coupling reaction, N,N-dicyclohexylcarbodiimide (AAPPTec, KY, USA)/ 6-chloro-1-hydroxy-benzotriazole (AAPPTec, KY, USA) (0.20/0.21 mmol) was applied to preactivate Fmoc-amino acids (AAPPTec, KY, USA) (0.21 mmol) in N,N-dimethylformamide at room temperature. (c) A cleavage cocktail containing trifluoroacetic acid (TFA)/H₂O/triisopropylsilane (Sigma-Aldrich, MO, USA)/1,2-ethanedithiol (Sigma-Aldrich, MO, USA) (93/2/2.5/2.5 v/v/v) was used for side chain deprotection reactions and peptide separation from the resin. (d) Cool diethyl ether (Sigma-Aldrich, MO, USA) was used for precipitation of crude peptides, which was dried at room temperature and analysed using RP-HPLC analytical chromatography.

Pei J., Xiong L., Chu M., Guo X, & Yan P. (2020). Effect of intramolecular disulfide bond of bovine lactoferricin on its molecular structure and antibacterial activity against Trueperella pyogenes separated from cow milk with mastitis. BMC Veterinary Research, 16, 401.

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Radiolabeled PEGylated Peptide Synthesis

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Unsubstituted Wang, Fmoc-protected amino acids, N-hydroxybenzotriazole (HOBt), O-(benzotr1-yl)-N,N,N′,N′-tetramethyluronium hexa-fluorophosphate (HBTU), and N,N dimethylformamide (DMF) were from AAPPTec (Louisville, KY, U.S.A.). Trifluoroacetic acid (TFA) and acetonitrile were obtained from Fisher Scientific (Pittsburgh, PA, U.S.A.). Diisoproplyethylamine (DIPEA), piperidine, acetic anhydride, Sephadex G-25 and G-10 were purchased from Sigma Chemical Co. (St Louis, MO, U.S.A.). Maleimide- mPEG 2, 5, 10, 20 and 30 kDa were obtained from Laysan Bio (Arab, AL, U.S.A.). Sodium ¹²⁵Iodine was obtained from Perkin Elmer (Waltham, MA, U.S.A.). Pierce iodogen tubes were obtained from ThermoFisher (Waltham, MA, U.S.A.). pGL3 control vector, a 5.3-kbp luciferase plasmid containing a SV40 promoter and enhancer, was obtained from Promega (Madison, WI, U.S.A.). pGL3 was amplified in a DH5α strain of Escherichia coli and purified using a Qiagen giga prep (Germantown, MD) according to the manufacturer’s instructions.

Allen R.J., Mathew B, & Rice K.G. (2018). PEG-Peptide Inhibition of Scavenger Receptor Uptake of Nanoparticles by the Liver. Molecular pharmaceutics, 15(9), 3881-3891.

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Peptide Synthesis and Plasmid Purification

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Unsubstituted Wang resin and diisopropylcarbodiimide (DIC) were from Advanced ChemTech (Louisville, KY, USA). Fmoc-protected amino acids, N-hydroxybenzotriazole (HOBt), O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU), and N, N-dimethylformamide (DMF) were from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA) and acetonitrile were obtained from Fisher Scientific (Pittsburgh, PA, USA). Diisoproplyethylamine, piperidine and acetic anhydride were purchased from Sigma Chemical Co. (St Louis, MO, USA). Maleimide-mPEG_30kDa was obtained from Laysan Bio (Arab, AL, USA). D-Luciferin and luciferase from Photinus pyralis were obtained from Roche Applied Science (Indianapolis, IN, USA). pGL3 control vector, a 5.3-kbp luciferase plasmid containing a SV40 promoter and enhancer, was obtained from Promega (Madison, WI, USA). pGL3 was amplified in a DH5α strain of Escherichia coli and purified using a Qiagen giga prep according to the manufacturer’s instructions.

Baumhover N.J., Duskey J.T., Khargharia S., White C.W., Crowley S.T., Allen R.J, & Rice K.G. (2015). The Structure-Activity Relationship of PEGylated Polylysine Peptides as Scavenger Receptor Inhibitors for Non-Viral Gene Delivery. Molecular pharmaceutics, 12(12), 4321-4328.

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