The phenolic composition and glutathione content were measured with liquid chromatography high-resolution mass spectrometry (LC-HRMS) following the method described by Fia et al. [21 (link)]. A chromatograph Accela 1250 (Thermo Fisher Scientific, Waltham, MA, USA), a Kinetex F5 column (2.1 × 100 mm 1.7 μm-Phenomenex (Torrance, CA, USA)) and an LTQ OrbitrapExactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) were used. The quantitative analysis was performed with TraceFinderTM 4.1 software (Thermo Fisher Scientific, Waltham, MA, USA) following an external standard method, using a linear regression from 0.05 to 1 g/L of five standard solutions.
Ltq orbitrapexactive mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The LTQ Orbitrap Exactive mass spectrometer is a high-resolution, accurate-mass (HRAM) instrument designed for a wide range of analytical applications. It combines the high-resolution and mass accuracy of the Orbitrap mass analyzer with the sensitivity and robustness of the LTQ linear ion trap. The instrument is capable of performing full-scan MS and MS/MS analysis with accurate mass measurement.
Automatically generated - may contain errors
3 protocols using ltq orbitrapexactive mass spectrometer
1
Phenolic and Glutathione Quantification
2
Phenolic and Glutathione Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The phenolic composition and glutathione content were measured with liquid chromatography-high-resolution mass spectrometry (LC-HRMS) following Fia et al. [22 (link)], using a chromatograph Accela 1250 (Thermo Fisher Scientific, Waltham, MA, USA) and a Kinetex F5 column (2.1 × 100 mm 1.7 µm—Phenomenex (Torrance, CA, USA)). An LTQ OrbitrapExactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was used. The quantitative analysis was performed with the TraceFinder™ 4.1 software (Thermo Fisher Scientific, Waltham, MA, USA) with an external standard method, using a linear regression from 0.05 to 1 g/L of five standard solutions.
3
Phenolic Compounds and Glutathione Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analysis of the phenolic compounds and glutathione was performed via liquid chromatographyhigh-resolution mass spectrometry (LC-HRMS), according to Fia et al. (2018) using an Accela 1250 (Thermo Fisher Scientific) coupled with an LTQ OrbitrapExactive mass spectrometer (Thermo Fisher Scientific) equipped with an electrospray ionization (ESI) source in negative mode.
The standards were purchased from Sigma-Aldrich (Milan, Italy), except for the quercetin 3-O-glucoside which was supplied by Analytik GmbH (Rülzheim, Germany). Coumaric and ferulic acids were used as standards for coutaric and fertaric acids due to the lack of reference materials.
Data were expressed as mg of phenols/kg of the UGs or food models.
The standards were purchased from Sigma-Aldrich (Milan, Italy), except for the quercetin 3-O-glucoside which was supplied by Analytik GmbH (Rülzheim, Germany). Coumaric and ferulic acids were used as standards for coutaric and fertaric acids due to the lack of reference materials.
Data were expressed as mg of phenols/kg of the UGs or food models.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!