Tri rna isolation reagent

The total RNA of splenic Th1 cells was extracted using the TRI RNA isolation reagent (Sigma). Quantitative PCR with specific primers was performed using the Power SYBR Green kit and the StepOnePlus instrument (all from Applied Biosystems). The primers are as follows:
mTOR:
GAPDH

Pancreatic CD11b⁺CD11c⁻ cells were isolated using CD11c and CD11b magnetic microbeads (Miltenyi) respectively. Total RNA was extracted using TRI RNA isolation reagent (Sigma-Aldrich). Quantitative PCR for the targets of interest was performed using Power SYBR Green kit and the StepOnePlus instrument (all from Applied Biosystems). The primers used were:
Arg1
Forward: 5′-GATTATCGGAGCGCCTTTCT-3′
Reverse: 5′-CCACACTGACTCTTCCATTCTT-3′
FIZZ1
Forward: 5′-TCCCAGTGAATACTGATGAGA-3′
Reverse: 5′-CCACTCTGGATCTCCCAAGA-3′
CD206
Forward: 5′-CTGCAGATGGGTGGGTTATC-3′
Reverse: 5′-GGCATTGATGCTGCTGTTATG-3′
GAPDH
Forward: 5′-AACTTTGGCATTGTGGAAGG-3′
Reverse: 5′-GGATGCAGGGATGATGTTCT-3′
TNFα
Forward: 5′-TCTCATGCACCACCATCAAGGACT-3′
Reverse: 5′-TGACCACTCTCCCTTTGCAGAACT-3′,
IL-1β
Forward: 5′-AGCTGAAAGCTCTCCACCTCAA-3′
Reverse: 5′-TGTCGTTGCTTGGTTCTCCTTG-3′
IL-12Rβ2
Forward: 5′-TGTGGGGTGGAGATCTCAGT-3′
Reverse: 5′-TCTCCTTCCTGGACACATGA-3′.