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2 protocols using coenzyme q9

1

Coenzyme Q Metabolism and Amyloid-beta

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Coenzyme Q2 and Q6 were purchased from Sigma Aldrich (#C8081, #C9504). Coenzyme Q9 was purchased from Cayman Chemical (#16866). Coenzyme Q10 was generously provided by Kaneka Corporation. Human Aβ25–35 (#AS-24227) and Human Aβ25–35 HiLyte™ Fluor 488-labeled (#AS-63308) were obtained from Anaspec. The fluorescent probes, Fluo-4 AM (#F23917), H2DCF-DA (#C6827), MitoSOX (#M36008), MitoTracker Red CMXRos (#M7512), Calcein-AM (#C34852), and Hoescht 33258 (#861405), were obtained from Thermo Fisher. Ethidium bromide (EtBr, #46067) was acquired from Sigma–Aldrich.
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2

Quantifying Oxidative Stress with Lipid Peroxidation

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Ubiquinol was purchased from Sigma Aldrich (USP analytical standard, St. Louis, MO). Coenzyme Q10 and alpha-, beta-, and gamma cyclodextrin (α-, β-, and γ-CD, respectively) were purchased from TCI America (Portland, OR). Dihydroethidium (DHE)-based assay kits were purchased from Abcam (Cambridge, MA), and used according to the manufacturer’s protocol. Coenzyme Q9 was purchased from Cayman Chemicals (Ann Arbor, MI). C11-Bodipy 581/591 lipid peroxidation assay kits were purchased from Thermo Fisher (Waltham, MA). All other reagents and solvents were at least of analytical grade and used as received.
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