Isoprostanes’ standards were from Cayman Chemical Company (Ann Arbor, Michigan, USA) (15(R)-15-F2t-IsoP, PGE2, 2,3-dinor-iPF2 αIII, 15-keto-15-E2t-IsoP, 15-keto-15-F2t-IsoP, 15-E2t-IsoP, 15-F2t-IsoP, 5-F2t-IsoP, PGF2α, 1a,1b-dihomo-PGF2α), or synthesized at the Institute of Biomolecules Max Mousseron (IBMM) (Montpellier, France) by Dr Durand’s team (4(RS)-F4t-NeuroP, 10-epi-10-F4t-NeuroP, 14(RS)-14-F4t-NeuroP, Ent-7(RS)-F2t-dihomo-IsoP, 17-F2t-dihomo-IsoP, 17-epi-17-F2t-dihomo-IsoP, 17(RS)-10-epi-SC-Δ15-11-dihomo-IsoF, 7(RS)-ST-Δ8-11-dihomo-IsoF) [28 (link)].
15 keto 15 e 2t isop
Manufactured by Cayman Chemical
Sourced in United States
15-keto-15-E 2t -IsoP is a laboratory standard used for research purposes. It serves as a reference compound for analytical procedures. The product's core function is to provide a reliable and consistent reference point for the identification and quantification of related analytes in scientific investigations.
Automatically generated - may contain errors
Lab products found in correlation
15 f2t isop, by Cayman Chemical (3 mentions)
15 keto 15 f 2t isop, by Cayman Chemical (3 mentions)
5 f2t isop, by Cayman Chemical (2 mentions)
2 3 dinor 15 epi 15 f2t isop, by Cayman Chemical (2 mentions)
15 r 15 f2t isop, by Cayman Chemical (2 mentions)
15 e 2t isop, by Cayman Chemical (2 mentions)
Ethyl acetate, by ITW Reagents (2 mentions)
Pgf2α, by Cayman Chemical (1 mentions)
Pgf2α d4, by Cayman Chemical (1 mentions)
Pgf2a d4, by Cayman Chemical (1 mentions)
Potassium hydroxide koh, by Merck Group (1 mentions)
Heptane, by Avantor (1 mentions)
Methanol ch3oh, by Avantor (1 mentions)
Acetonitrile ch3cn, by Avantor (1 mentions)
Milli q system, by Merck Group (1 mentions)
Glacial acetic acid, by ITW Reagents (1 mentions)
Microvue creatinine eia, by Quidel (1 mentions)
Chlorhydric acid, by ITW Reagents (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
5 protocols using 15 keto 15 e 2t isop
1
Isoprostanes Standards and Synthesis
2
Quantification of Isoprostane and Neurosteroid Biomarkers
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The isoprostanes’ standards 5-F2t-IsoP, 2,3-dinor-15-epi-15-F2t-IsoP, 15(R)-15-F2t-IsoP, 15-F2t-IsoP, 15-E2t-IsoP, 15-keto-15-E2t-IsoP, 15-keto-15-F2t-IsoP, prostaglandins PGE2, PGF2α, and 1a,1b-dihomo-PGF2α and deuterated internal standard (IS) PGF2α-d4 were from Cayman Chemical Company (Ann Arbor, Michigan, USA). The standards of 10-epi-10-F4t-NeuroP, d4-10-epi-10-F4t-NeuroP, 4(RS)-4-F4t-NeuroP, 14(RS)-14-F4t-NeuroP, 7(RS)-ST-Δ8-11-dihomo-IsoF, 17(RS)-10-epi-SC-Δ15-11-dihomo-IsoF, 17-epi-17-F2t-dihomo-IsoP, 17-F2t-dihomo-IsoP, and ent-7(RS)-7-F2t-dihomo-IsoP, were synthesized at the Institute of Biomolecules Max Mousseron (IBMM) (Montpellier, France) by Professor Durand’s team25 (link).
The calibration curves were prepared by serial dilutions in H2O (pH 3):CH3OH (85:15 v/v) with CH3COOH 0.01%, in concentrations from 300 nmol L−1 to 0.004 nmol L−1 of each analyte.
The calibration curves were prepared by serial dilutions in H2O (pH 3):CH3OH (85:15 v/v) with CH3COOH 0.01%, in concentrations from 300 nmol L−1 to 0.004 nmol L−1 of each analyte.
3
Quantifying Eicosanoids and Docosanoids
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Commercially available IsoPs (2,3-dinor-15-F 2t -IsoP, 15-keto-15-E 2t -IsoP, 15-keto-15-F 2t -IsoP, 15-epi-15-F 2t -IsoP, 15-E 2t -IsoP, 15-F 2t -IsoP), prostaglandins (PGs) (PGE 2 , PGF 2a , 2,3-dinor-11b-PGF 2a , 11b-PGF2a, 6-keto-PGF 1a ), dihomo-PG (1a,1b-dihomo-PGF 2a ) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA), with purities !95% as well as deuterated internal standards (IS, PGF 2a -d 4 and 15-F 2t -IsoP-d 4 ) with purities !98% and incorporation !99% deuterated form (d 1 -d 4 ); <1% d 0 .
Other analytical standards employed were F 2 -IsoPs (5-F 2t -IsoP, 5-epi-5-F 2t -IsoP, 15-epi-2,3-dinor-15-F 2t -IsoP), F 4 -NeuroPs (4-F 4t -NeuroP, 4-epi-4-F 4t -NeuroP, 10-epi-10-F 4t -NeuroP, 10-F 4t -NeuroP, 14(RS)-14-F 4t -NeuroP), Potassium hydroxide (KOH) was from Sigma Aldrich Química SA (Madrid, Spain). LC-MS grade solvents (methanol CH 3 OH, ethanol, heptane and acetonitrile CH 3 CN) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Formic acid (HCOOH, 98%) and ethyl acetate (analytical grade) were from Panreac (Barcelona, Spain). Ultrapure H 2 O was generated on a milliQ system from Merck Millipore (Darmstadt, Germany).
Other analytical standards employed were F 2 -IsoPs (5-F 2t -IsoP, 5-epi-5-F 2t -IsoP, 15-epi-2,3-dinor-15-F 2t -IsoP), F 4 -NeuroPs (4-F 4t -NeuroP, 4-epi-4-F 4t -NeuroP, 10-epi-10-F 4t -NeuroP, 10-F 4t -NeuroP, 14(RS)-14-F 4t -NeuroP), Potassium hydroxide (KOH) was from Sigma Aldrich Química SA (Madrid, Spain). LC-MS grade solvents (methanol CH 3 OH, ethanol, heptane and acetonitrile CH 3 CN) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Formic acid (HCOOH, 98%) and ethyl acetate (analytical grade) were from Panreac (Barcelona, Spain). Ultrapure H 2 O was generated on a milliQ system from Merck Millipore (Darmstadt, Germany).
4
Quantification of Isoprostanes and Prostaglandins
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Isoprostanes' standards of 15(R)-15-F 2t -IsoP, 2,3-dinor-15-epi-15-F 2t -IsoP, 5-F 2t -IsoP, 15-keto-15-E 2t -IsoP, 15-keto-15-F 2t -IsoP, 15-E 2t -IsoP, 15-F 2t -IsoP, the prostaglandins PGE 2 and PGF 2α , as well as the deuterated internal standard (IS) PGF 2α -d 4 were purchased from Cayman Chemical Company (Ann Arbor, Michigan, USA). The standards of 7(RS)-ST-Δ 8 -11-dihomo-IsoF, 10-epi-10-F 4t -NeuroP, d 4 -10epi-10-F 4t -NeuroP, 4(RS)-F 4t -NeuroP, 17-epi-17-F 2t -dihomo-IsoP, 17-F 2t -dihomo-IsoP, 17(RS)-10-epi-SC-Δ 15 -11-dihomo-IsoF, Ent-7(RS)-7-F 2t -dihomo-IsoP, and 14(RS)-14-F 4t -NeuroP were synthetized by Durand's team at the Institute des Biomolécules Max Mousseron (IBMM) (Montpellier, France) (Fig. S1 in Electronic Supplementary material) [34] . Methanol (LC-MS grade) and n-heptane (analytical grade) were obtained from J.T. Baker (Avantor Performance Materials B.V., The Netherlands). Chlorhydric acid and acetic acid glacial (99.5%) were purchased from Panreac (Castellar del Vallés, Barcelona, Spain). Ammonium acetate, sodium acetate, sodium hydroxide, and β-glucuronidase (type H2, from Helix pomatia) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Creatinine assay (MicroVue creatinine EIA) from Quidel Corporation (San Diego, CA, USA).
5
Quantitative Lipid Mediator Analysis
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Some compounds were acquired from Cayman Chemical Company (Ann Arbor, Michigan, USA) (PGE 2 , PGF 2␣ , 1a,1b-dihomo-PGF 2␣ , 5-F 2t -IsoP, 15-E 2t -IsoP, 15-F 2t -IsoP, 15-keto-15-E 2t -IsoP, 15-keto-15-F 2t -IsoP, 15(R)-15-F 2t -IsoP, 2,3-dinor-15-epi-15-F 2t -IsoP, PGF 2␣ -D 4 ) and used as analytes, as in a previous study [27] . Other compounds nor available commercially were synthesized ((4(RS)-F 4t -NeuroP, 7(RS)-ST-8 -11-dihomo-IsoF, Ent-7(RS)-7-F 2t -dihomo-IsoP, 10-epi-10-F 4t -NeuroP, 14(RS)-14-F 4t -NeuroP, 17-epi-17-F 2t -dihomo-IsoP, 17-F 2t -dihomo-IsoP, 17(RS)-10-epi-SC-15 -11-dihomo-IsoF, D 4 -10-epi-10-F 4t -NeuroP) [28] , and they were used as analytes, as in previous studies [29, 30] .
Methanol (CH 3 OH, HPLC grade), acetonitrile (CH 3 CN, HPLC grade), and glacial acetic acid were from J.T. Baker (Avantor Performance Materials B.V., The Netherlands). Ethyl acetate (analytical grade) was from Panreac (Barcelona, Spain). Protein assay kit (Pierce TM BCA) was purchased from Thermo Scientific (Rockford, USA).
Methanol (CH 3 OH, HPLC grade), acetonitrile (CH 3 CN, HPLC grade), and glacial acetic acid were from J.T. Baker (Avantor Performance Materials B.V., The Netherlands). Ethyl acetate (analytical grade) was from Panreac (Barcelona, Spain). Protein assay kit (Pierce TM BCA) was purchased from Thermo Scientific (Rockford, USA).
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