Rpmi 1640 medium with l glutamine

All chemicals and solvents were analytical grade. Antibiotic antimycotic solution (100×) stabilized, diethyl polycarbonate (DEPC), dimethyl sulfoxide (DMSO), Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), glycine buffer solution, Griess reagent, guanidine thiocyanate, lipopolysaccharide (LPS) from Escherichia coli O26:B6 smooth strain, n-hexane, nystatin, RNAzol^® RT, RPMI-1640 medium with L-glutamine, SYBR^® Green Quantitative RT-qPCR kit, and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). GoTaq^® qPCR Master Mix kit and ImProm-II™ Reverse Transcription System reverse transcription kit was purchased from Promega (Promega^® Corporation, Madison, WI, USA).

Annexin V FITC and PI, and annexin V binding buffer 10× were provided by Immunostep SL. (Salamanca, Spain). Dimethyl sulfoxide (DMSO) was obtained from Merck (Darmstadt, Germany). RPMI 1640 medium with l-glutamine, DMEM medium with l-glutamin, bovine serum albumin (BSA), trypan blue (TB), urea, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma (St Louis/MO, USA). RC DC™ Protein Assay was purchased from BioRad (Hercules/CA, USA). 96-well plates, Coomassie Brilliant Blue solution, Click-IT™ AHA (l-Azidohomoalanine) and Click-iT™ Protein Enrichment Kit, for click chemistry capture of azide-modified proteins were obtained from Thermo Scientific (Rockford/IL, USA). Cycloscope™ Reagent for cell cycle analysis was purchased from Cytognos (Salamanca, Spain). Heat inactivated fetal bovine serum (FBS), l-glutamine, penicillin–streptomycin (P-S) and 0.25% trypsin–EDTA and DMEM, high glucose, no glutamine, no methionine, no cystine were purchased from Gibco®(Gran Island/NY, USA). 12-well clear flat bottom plates were purchased from Corning (Corning/NY, USA).