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Single cell 3 rnaseq technology

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The 10x Single Cell 3' RNAseq technology is a laboratory instrument designed for the analysis of single-cell gene expression. It enables the capture and barcoding of individual cells, allowing for the high-throughput sequencing and quantification of their transcriptomes.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (4 mentions) Agilent high sensitive dna kit, by Agilent Technologies (2 mentions) Nextseq 2000, by Illumina (2 mentions) Tryple, by Thermo Fisher Scientific (2 mentions) Cellometer automated cell counter, by Revvity (2 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)

2 protocols using single cell 3 rnaseq technology

1

Single-cell RNA-seq of DHT-treated human islets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human islets (750 IEQ per condition) were treated with either 10nM DHT or EtOH overnight in a humidified incubator containing 4% CO2 at 37°C. Islet cells were then dispersed using TrypLE (Thermofischer), and immediately evaluated for viability (89.88 ± 1.77%) by Cellometer Automated Cell Counter (Nexcelom Bioscience) prior to single cell RNAseq library preparation. For 10x single cell RNAseq library preparation, 5000 individual live cells per sample were targeted by using 10x Single Cell 3′ RNAseq technology provided by 10x Genomics (10X Genomics Inc). Briefly, viable single cell suspensions were partitioned into nanoliter-scale Gel Beads-In-EMulsion (GEMs). Full-length barcoded cDNAs were then generated and amplified by PCR to obtain sufficient mass for library construction. Following enzymatic fragmentation, end-repair, A-tailing, and adaptor ligation, single cell 3′ libraries comprising standard Illumina P5 and P7 paired-end constructs were generated. Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent) and quantified by Qubit 2.0 fluorometer (ThermoFisher). Pooled libraries at a final concentration of 750pM were sequenced with paired end single index configuration by Illumina NextSeq 2000 (Illumina).
Xu W., Qadir M.M., Nasteska D., de Sa P.M., Gorvin C.M., Blandino-Rosano M., Evans C.R., Ho T., Potapenko E., Veluthakal R., Ashford F.B., Bitsi S., Fan J., Bhondeley M., Song K., Sure V.N., Sakamuri S.S., Schiffer L., Beatty W., Wyatt R., Frigo D.E., Liu X., Katakam P.V., Arlt W., Buck J., Levin L.R., Hu T., Kolls J., Burant C.F., Tomas A., Merrins M.J., Thurmond D.C., Bernal-Mizrachi E., Hodson D.J, & Mauvais-Jarvis F. (2023). Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells. Cell reports, 42(5), 112529.
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2

Single-cell RNA-seq of DHT-treated human islets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human islets (750 IEQ per condition) were treated with either 10nM DHT or EtOH overnight in a humidified incubator containing 4% CO2 at 37°C. Islet cells were then dispersed using TrypLE (Thermofischer), and immediately evaluated for viability (89.88 ± 1.77%) by Cellometer Automated Cell Counter (Nexcelom Bioscience) prior to single cell RNAseq library preparation. For 10x single cell RNAseq library preparation, 5000 individual live cells per sample were targeted by using 10x Single Cell 3′ RNAseq technology provided by 10x Genomics (10X Genomics Inc). Briefly, viable single cell suspensions were partitioned into nanoliter-scale Gel Beads-In-EMulsion (GEMs). Full-length barcoded cDNAs were then generated and amplified by PCR to obtain sufficient mass for library construction. Following enzymatic fragmentation, end-repair, A-tailing, and adaptor ligation, single cell 3′ libraries comprising standard Illumina P5 and P7 paired-end constructs were generated. Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent) and quantified by Qubit 2.0 fluorometer (ThermoFisher). Pooled libraries at a final concentration of 750pM were sequenced with paired end single index configuration by Illumina NextSeq 2000 (Illumina).
Xu W., Qadir M.M., Nasteska D., de Sa P.M., Gorvin C.M., Blandino-Rosano M., Evans C.R., Ho T., Potapenko E., Veluthakal R., Ashford F.B., Bitsi S., Fan J., Bhondeley M., Song K., Sure V.N., Sakamuri S.S., Schiffer L., Beatty W., Wyatt R., Frigo D.E., Liu X., Katakam P.V., Arlt W., Buck J., Levin L.R., Hu T., Kolls J., Burant C.F., Tomas A., Merrins M.J., Thurmond D.C., Bernal-Mizrachi E., Hodson D.J, & Mauvais-Jarvis F. (2023). Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cells. Cell reports, 42(5), 112529.
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