MBHA resin (0.79 meq/g; Peptides International) was swelled in DMF (8–10 mL) with DIEA (650 μL). This was then coupled with Boc-Leu (2 mMol; 4 mL 0.5 M HBTU; 347 μL DIEA; 40 min.), washed with DMF then DCM. Peptidyl-resin Boc deprotection was achieved with 100% Trifluoroacetic acid (TFA; 5 min., ×2; Fisher Scientific), then flow-washed with DMF (1 min., ×2), followed by coupling with 3,3′dithiodiprionic acid (2 mMol; 8 mL of 0.5 M HBTU in DMF; 1 mL of DIEA; 40 min.). To counteract ester formation the resin-linker was washed with DMF and treated with ethanolamine (650 μL; Alfa Aesar) and DIEA (200 μL; 5 mL; in DMF; 40 min.). This was then flow-washed with DMF (1 min., ×2) and treated with 2-mercaptoethanol (Sigma-Aldrich) (650 μL; 5mL of DMF; 100μL DIEA; 60 min.). Upon peptidyl-resin reduction, the resin was flow-washed with DMF (1 min., ×2) and coupled with Boc-Cys(4-MeOBzl) (40 min.), as described above. The resulting pre-loaded Boc-Cys(4-MeOBzl) thioester linker resin (Boc-Cys(4-MeOBzl)-MPAL resin) was then used in the production of N-terminal peptide fragments for NCL at approximately 0.5 mMole scale [29 (link)].
Mbha resin
Manufactured by Peptide Institute
MBHA resin is a solid-phase resin used in peptide synthesis. It serves as a support matrix for the attachment and subsequent elongation of peptide chains. The resin provides a stable platform for chemical reactions involved in peptide assembly.
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Lab products found in correlation
2 protocols using mbha resin
1
Cysteine-Containing Peptide Synthesis
2
Synthesis and Characterization of PNA Oligomers
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[2,2':6',2''-terpyridine]-4'-carboxylic acid was purchased from Alfa Aesar and used without further purification. The PNA oligomers were prepared by solidphase peptide synthesis on an MBHA resin (Peptides International) downloaded with l-lysine to an 0.1 meq./g NH 2 content. Boc/Z-protected PNA monomers were purchased from PolyOrg and used without further purification. The PNA oligomers were cleaved from the solid support using a mixture of m-cresol/thioanisole/TFMSA/ TFA (1:1:2:6) for 1 h. Cleaved PNA was precipitated using diethyl ether and purified by reversed-phase HPLC using a C18 silica column on a Waters 600. Absorbance was measured with a Waters 2996 photodiode array detector. Characterization of the oligomers was done by MALDI-ToF mass spectrometry using α-cyano-4hydroxycinnamic acid matrix (10 mg/ mL in 1:1 water/acetonitrile, 0.1% TFA). An Applied Biosystems Vo yager workstation with delayed extraction was used for MALDI-ToF mass spectrometry. The calculated and experimental values of m/z for [PNA + H + ] species are provided in Table 2.
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