Anti mouse igg horseradish peroxidase hrp conjugated goat a2304

Manufactured by Merck Group

Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304) is a laboratory reagent that consists of horseradish peroxidase enzyme conjugated to goat-derived antibodies that specifically bind to mouse immunoglobulin G (IgG) antibodies. This product is commonly used as a secondary antibody in immunoassay techniques, such as Western blotting and enzyme-linked immunosorbent assay (ELISA), to detect and quantify the presence of mouse IgG in various sample types.

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Lab products found in correlation

Anti rabbit igg hrp conjugated goat a0545, by Merck Group (4 mentions) Avidin biotinylated peroxidase complex detection system abc kit, by Vector Laboratories (4 mentions) Alexa fluor 594 conjugated goat polyclonal anti mouse igg, by Thermo Fisher Scientific (3 mentions) 3 3 diaminobenzidine tetrahydrochloride dab, by Merck Group (2 mentions) Alexa fluor 488 conjugated goat polyclonal anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Human chorionic gonadotropin (hcg), by Organon (2 mentions) Dab ni, by Vector Laboratories (1 mentions) Metformin, by Merck Group (1 mentions) Humulin nph, by Eli Lilly (1 mentions) Flutamide, by Merck Group (1 mentions) Diaminobenzidine dab, by Merck Group (1 mentions) Ar sc 816, by Santa Cruz Biotechnology (1 mentions) P21cip1, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Horseradish peroxidase (696 citations) Horseradish peroxidase (HRP) (213 citations) Goat anti-mouse-HRP (44 citations) Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (35 citations) HRP-conjugated goat anti-mouse (15 citations) A2304 (11 citations) HRP anti-mouse (6 citations) Anti-IgG mouse (5 citations) Horseradish peroxidase (HRP)-anti-mouse (2 citations) HRP- anti-goat (2 citations)

4 protocols using anti mouse igg horseradish peroxidase hrp conjugated goat a2304

Immunostaining and Western Blot Protocol

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Metformin, 3,3-diaminobenzidine tetrahydrochloride (DAB), and anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304) and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG was purchased from Invitrogen (Sollentuna, Sweden). The avidin-biotinylated-peroxidase complex detection system (ABC kit) and DAB-Ni were purchased from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot analysis and immunostaining in the present study, along with their species, molecular weight, dilutions, and sources are listed in Supplemental Table 1.

Wang T., Zhang J., Hu M., Zhang Y., Cui P., Li X., Li J., Vestin E., Brännström M., Shao L.R, & Billig H. (2019). Differential Expression Patterns of Glycolytic Enzymes and Mitochondria-Dependent Apoptosis in PCOS Patients with Endometrial Hyperplasia, an Early Hallmark of Endometrial Cancer, In Vivo and the Impact of Metformin In Vitro. International Journal of Biological Sciences, 15(3), 714-725.

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Immunohistochemical Analysis of Hormone Receptors

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Human chorionic gonadotropin (hCG) was from NV Organon (Oss, Holland). 17β-estradiol (E2), progesterone (P4), and 3,3-Diaminobenzidine tetrahydrochloride (DAB) were from Sigma-Aldrich (St. Louis, MO). The Avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). All antibodies were from the following sources: GLUT4 (ab33780) antibody was from Abcam (Cambridge, UK), cytokeratin 8/18 (#4546) antibody was from Cell Signaling Technology (Danvers, MA); estrogen receptor alpha (ERα, #6F11) and progesterone receptor A/B (PRA and PRB, #16) antibodies were from Novocastra Laboratories (Newcastle, UK); and estrogen receptor beta (ERβ, PPG5/10) antibody was from AbD Serotec (Oxford, UK). β-actin (P-0130), anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich.

Cui P., Li X., Wang X., Feng Y., Lin J.F., Billig H, & Shao R. (2015). Lack of cyclical fluctuations of endometrial GLUT4 expression in women with polycystic ovary syndrome: Evidence for direct regulation of GLUT4 by steroid hormones. BBA Clinical, 4, 85-91.

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Immunohistochemical and Western Blot Analyses

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hCG was from NV Organon (Oss, Holland). Human recombinant insulin (Humulin NPH) was from Eli Lilly (Lilly Pharmaceuticals, Giza, Egypt). 3,3-diaminobenzidine tetrahydrochloride (DAB) was from Sigma-Aldrich (St. Louis, MO). The avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot and immunohistochemical analyses in the present study, their dilution, and sources are listed in Supplemental Table 1. Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG and Alexa Fluor 488-conjugated goat polyclonal anti-rabbit IgG were from Invitrogen (UK).

Zhang Y., Sun X., Sun X., Meng F., Hu M., Li X., Li W., Wu X.K., Brännström M., Shao R, & Billig H. (2016). Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus. Scientific Reports, 6, 30679.

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Molecular Mechanisms of Androgen Receptor Regulation

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DHT (a specific AR agonist, D-5027), flutamide (a specific AR antagonist, F-0397), and diaminobenzidine (DAB) were from Sigma-Aldrich (St. Louis, MO). Avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). All antibodies were from the following sources: AR (#5153, this antibody reacts with human tissues), AMPKα (#2532), phospho-AMPKα (phosphorylated threonine 172, #2535), and Ki-67 (#11882) antibodies were from Cell Signaling Technology (Danvers, MA); AR (sc-816, this antibody reacts with rodent tissues) and p21^Cip1 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA); and cysteine-rich protein 61 (CYR61, HPA029853) antibody was from Sigma-Aldrich. Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304) and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG, and Alexa Fluor 488-conjugated goat polyclonal anti-rabbit IgG were from Invitrogen (Life Technologies Europe BV, Stockholm, Sweden).

Li X., Pishdari B., Cui P., Hu M., Yang H.P., Guo Y.R., Jiang H.Y., Feng Y., Billig H, & Shao R. (2015). Regulation of Androgen Receptor Expression Alters AMPK Phosphorylation in the Endometrium: In Vivo and In Vitro Studies in Women with Polycystic Ovary Syndrome. International Journal of Biological Sciences, 11(12), 1376-1389.

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