The fasting plasma glucose, triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), apolipoprotein-A (ApoA), and apolipoprotein-B (ApoB) in serum were determined with a Hitachi 7600-020 automatic biochemical analyzer (Hitachi, Tokyo, Japan). Fibrinogen was measured using a Sysmex CA-1500 automated coagulation analyzer (Sysmex, Kobe, Japan). High-sensitivity CRP (hs-CRP, BlueGene, Shanghai, China) and IL-6 (Boster) were quantified using ELISA kits obtained commercially.
7600 020 automatic biochemical analyzer
Manufactured by Hitachi
Sourced in Japan
The 7600-020 automatic biochemical analyzer is a laboratory instrument designed to perform automated biochemical tests and analyses. It is capable of processing samples and providing quantitative measurements of various biochemical markers, such as enzymes, proteins, and metabolites. The core function of this analyzer is to streamline the analytical process, delivering accurate and reliable results in a consistent manner.
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Lab products found in correlation
Advia 2120 hematology analyzer, by Siemens (1 mentions)
Ca 7000 system, by Sysmex (1 mentions)
E170 immunoassay analyzer, by Roche (1 mentions)
Hcy reagent, by Beijing Strong Biotechnologies (1 mentions)
Unicel dxi 800 access immunoassay system, by Beckman Coulter (1 mentions)
Total antioxidant status kit, by Randox (1 mentions)
Advia centaur xp, by Siemens (1 mentions)
Immulite 2000, by Siemens (1 mentions)
10 protocols using 7600 020 automatic biochemical analyzer
1
Comprehensive Metabolic and Inflammatory Profiling
2
Dialysis Adequacy and Protein Intake
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Blood samples were obtained at the first dialysis session of May 2012. All assays were performed in the hospital laboratory using routine methods. Complete blood counts were measured using a ADVIA2120 hematology analyzer (Siemens, Munich, Germany). Biochemical parameters were measured using a Hitachi 7600-020 automatic biochemical analyzer (Hitachi Ltd., Tokyo, Japan). Normalized protein catabolic rate (nPCR) (g/kg/day), which is known to be a valid surrogate for dietary protein intake, was measured using kinetic modeling. Urea clearance (Kt/V) was used for evaluation of the adequacy of dialysis.
3
Pretreatment Plasma D-dimer and Albumin Levels in ESCC
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As a part of clinical routine examinations, pretreatment plasma D-dimer levels, serum albumin levels, and CRP levels were measured 24 h to 1 week before surgery. Plasma D-dimer values were analyzed by a latex-enhanced immunoturbidimetric assay and a Sysmex CA 7000 system (Sysmex Corporation, Japan) according to the manufacturer’s instructions. Serum albumin levels and CRP levels were measured by using a Hitachi 7600-020 automatic biochemical analyzer (Hitachi, Japan). The optimal cutoff values of D-dimer and albumin in our study were verified using Youden’s index (YI) from the receiver operating characteristic (ROC) curve for ESCC prediction. The survival status was inserted into the YI to define the cutoff value as described by Huang et al. [22 (link)] and Liu et al. [23 (link)]. A high level of preoperative plasma D-dimer was defined as ≥0.5 μg/mL, and a low level of preoperative serum albumin was defined as <43.8 g/L.
4
Lipid Profile Evaluation Protocol
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All participants provided blood samples, and 3 tubes of fasting venous blood were collected and were put respectively into centrifuge tubes which contained anticoagulant and no anticoagulant. The examined indexes included TG, TC, HDL-C, LDL-C and so on. The 7600–020 automatic biochemical analyzer which was made by Japan Hitachi Company was used for biochemical test of blood. Triglycerides test kits (1555-40-2009), High density lipoprotein cholesterol test kits (0967-40-2009) and Low density lipoprotein cholesterol test kits (0972-40-2009) which were produced by Shanghai Rongsheng biological pharmaceutical Co., LTD were used to test TG, HDL-C and LDL-C. Total cholesterol test kits (1555-40-2009) which were produced by Shanghai Kehua bio-engineering Co., LTD were used to test TC.
5
Comprehensive Blood Profiling Protocol
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Blood routine test was performed using BC-6800 blood cell analyzer (Mindray, Shenzhen, China) within 8 h after collection of anticoagulated whole blood. Serum samples were tested using the 7600-020 automatic biochemical analyzer (Hitachi, Tokyo, Japan) for the detection of high-sensitivity C-reactive protein (hCRP), total cholesterol (TC), triglyceride (TG), total protein (TP), albumin (ALB), globulin (GLO), albumin to globulin ratio (A/G), total bilirubin (TBIL), blood urea nitrogen (BUN), uric acid (UA), alanine aminotransferase (ALT), fasting blood glucose (FBS), and bile acid (TBA).
6
Serum Biomarker Evaluation Protocol
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Venous blood samples (10 ml) were taken from fasting patients and were centrifuged for 1 min at 1500 rpm, followed by collection of serum for the evaluation of Hcy, UA, FOA, and VitB12. Hcy reagent (Beijing Strong Biotechnologies, Inc., Beijing, China), and UA reagent (Pointe Biotech [Nanjing] Co., Ltd., Jiangsu, China) were used to detect the levels of Hcy and UA using an enzymatic cycling method with a Hitachi 7600-020 automatic biochemical analyzer (Hitachi, Tokyo, Japan). Chemiluminescence immunoassay was applied to detect the levels of FOA, VitB12, and other indicators with an E170 immunoassay analyzer (Hoffmann-La Roche Ltd., Basel, Switzerland).
7
Serum Vitamin and Homocysteine Levels
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Vitamin detector LINBIAO LK3000VI (Tianjin lanbiao electronic technology development co., LTD. China) and the original matching thiamin content determination kit were used to detect serum thiamin levels of two groups. Beckman Coulter’s UniCel DxI 800 Access Immunoassay System (Beckman Coulter, inc., United States) and the original folic acid assay kit were used to detect serum folic acid levels of two groups by immunoluminescence assay. The Hitachi 7600-020 automatic biochemical analyzer (Hitachi, Japan) and the corresponding homocysteine assay kit (Ningbo meicang biotechnology co., LTD. China) were used to detect serum homocysteine levels.
8
Colorimetric Determination of Total Antioxidant Status
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TAS was determined colorimetrically using the Total Antioxidant Status® kit (Randox Laboratories, Ltd., Crumlin, UK). In this assay, 2,2′-azino-di-3-ethylbenz-thiazoline sulfonate was incubated with a peroxidase (metmyoglobin) and hydrogen peroxide to produce the radical cation 2,2′-azino-di-3-ethylbenz-thiazoline sulfonate+. This forms a relatively stable blue-green color solution, which was measured using a 7600-020 automatic biochemical analyzer (Hitachi, Ltd., Tokyo, Japan) at 600 nm. The assay was calibrated with a 1.65 mmol/l Trolox standard. TAS results were expressed as mmol Trolox equivalent/l (mmol Trolox Eq./l).
9
Measuring Serum Oxidative Status
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TOS in rat serum was detected by xylenol orange method [24 (link)] and TAS in rat serum was detected by ABTS method [25 (link)]. The levels of TOS and TAS in serum were analyzed by a 7600-020 automatic biochemical analyzer (Hitachi, Japan).
10
Comprehensive Endocrine and Metabolic Evaluation
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Growth hormone stimulation test, IGF‐binding protein 3 test, and insulin‐like growth factor 1 (IGF‐1) test were performed on IMMULITE 2000 (Siemens, LOS Angeles, USA) by enhanced chemiluminescence immunoassay method. Thyroid function test was performed on ADVIA Centaur XP (Siemens, Munich, Germany) by microsome chemiluminescence method. Renal function test, liver function test, and electrolytes test were performed on Hitachi 7600‐020 automatic biochemical analyzer (Hitachi, Tokyo, Japan) by serial detecting method.
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