Activity of glutathione peroxidase was analyzed using commercially available kit RANSEL (Randox Laboratories, Crumlin, UK) and analyzer Randox RX Monza (Randox Laboratories, Crumlin, UK). The decrease in absorbance is measured at 340 nm. Enzyme activity was expressed as U/mg of total protein.
Ransel
Manufactured by Randox
Sourced in United Kingdom
RANSEL is a high-performance liquid chromatography (HPLC) system designed for analyzing and quantifying a wide range of compounds in various samples. It features advanced technology and precision engineering to deliver reliable and accurate results.
Automatically generated - may contain errors
Lab products found in correlation
Ransod, by Randox (8 mentions)
Ransod kit, by Randox (5 mentions)
Rx monza, by Randox (1 mentions)
Specord m40, by Zeiss (1 mentions)
Ultrospec 2100 pro uv vis, by Cytiva (1 mentions)
Access free t3, by Beckman Coulter (1 mentions)
Rx monza analyzer, by Randox (1 mentions)
Tbars assay kit, by Cayman Chemical (1 mentions)
Au400 analyser, by Olympus (1 mentions)
24 protocols using ransel
1
Glutathione Peroxidase Activity Assay
2
Quantitative Assessment of GPx1 and SOD1 in Whole Blood
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The quantitative determination of GPx1 in whole blood was performed using a commercial immunochemical kit (RANSEL; Randox Laboratories). Briefly, the heparinized whole blood was diluted with 1 mL of diluting agent and incubated for 5 minutes. After incubation, 1 mL of hemoglobin reagent (cyanide) was added in order to avoid falsely elevated results due to the presence of endogenous peroxidases. Finally, the samples were assayed within 20 minutes using an automated analyzer (BT2000; Biotecnica Instruments, Limena, Italy). The quantitative determination of SOD1 in whole blood was performed using a commercial immunochemical kit (RANSOD; Randox Laboratories). Briefly, 0.5 mL of heparinized whole blood samples was centrifuged for 10 minutes at 3,000 rpm in order to carefully wash the erythrocytes. Then, the erythrocytes were washed four times with 0.9% NaCl solution and centrifuged for 10 minutes at 3,000 rpm after each wash. Subsequently, the erythrocytes were made up to 2.0 mL with cold redistilled water, mixed, and left to stand at +4°C for 15 minutes. Finally, the lysate was diluted with sample diluent and assayed immediately using an automated analyzer (BT2000; Biotecnica Instruments) according to the manufacturer’s instructions.
3
Antioxidant Enzyme Assays in Samples
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4
Evaluating Oxidative Stress in Calves
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For oxidative stress evaluation, we analyzed TAS and GPx activity as indicators of Se status. Both variables were analyzed in 10 calves per group due to financial limitations, on days −7 and +60. Measurements were performed in duplicate, and they were repeated when the coefficient of variation was greater than 10%. Blood samples were obtained by jugular venipuncture early in the morning. Blood was collected in tubes containing sodium heparin and kept in a refrigerator (4–5 °C) until they were centrifuged within 6 h after collection. Subsequently, the samples were used to measure whole blood GPx activity and plasma TAS using the Ransel and TAS kits (Randox Laboratories, Crumlin, UK), respectively.
5
Glutathione peroxidase activity assay
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Glutathione peroxidase (GPx) activity was measured using a commercially available kit, RANSEL (Randox Laboratories, Poland), according to the manufacturer’s instructions. In this method, glutathione peroxidase catalyzes the oxidation of glutathione by cumene hydroperoxide. In the presence of glutathione reductase and NADPH, the oxidized glutathione is immediately converted to its reduced form with the concomitant oxidation of NADPH to NADP+. The decrease in absorbance at 340 nm was measured.
6
Antioxidant Enzyme Levels in Recurrent Aphthous Stomatitis
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The blood sample was analyzed for alterations in the levels of enzymatic antioxidants in recurrent aphthous stomatitis patients as compared to healthy controls. The level of SOD and GPx was estimated using commercially available kits RANSOD and RANSEL, respectively, by Randox Company Ltd., UK. Similar method for estimation of SOD and GPx was utilized by Momen-Beitollahi et al. [10 (link)]. The serum level of CAT was estimated using Beutler's E. method as applied by Çimen et al. [7 (link)].
7
Glutathione Peroxidase Activity Assay
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GPx activity was measured using a commercial kit (RANSEL®; Randox Lab, Antrim, United Kingdom). This assay is based on oxidation of GPx catalyses glutathiose (GSH) by cumene hydroperoxide. In presence of glutathione reductase (GR) and NADPH, the oxidized glutathione (GSSG) is immediately converted to the reduced form with concomitant oxidation of NADPH to NADP+. NADPH disappearance was measured at 340 nm and GPx activity was reported as percentage of control.
8
Antioxidant Enzyme Activity Assay
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SOD activity was determined using diagnostic kit RANSOD produced by RANDOX (Randox Laboratories Ltd., Crumlin, Country Antrim, UK) according to Arthur and Boyne [19 (link)] and expressed in U of SOD/10mgof protein.
GPx activity was determined using diagnostic kit RANSEL produced by RANDOX (Randox Laboratories Ltd., Crumlin, Country Antrim, UK) according to Paglia and Valentine [20 (link)] and expressed in U of GPx/mg of protein. Protein was measured using method of Bradford [21 (link)]. The assays were performed with the use of spectrophotometer SPECORD M40 (Carl Zeiss, Jena, Germany).
GPx activity was determined using diagnostic kit RANSEL produced by RANDOX (Randox Laboratories Ltd., Crumlin, Country Antrim, UK) according to Paglia and Valentine [20 (link)] and expressed in U of GPx/mg of protein. Protein was measured using method of Bradford [21 (link)]. The assays were performed with the use of spectrophotometer SPECORD M40 (Carl Zeiss, Jena, Germany).
9
Liver Antioxidant Assays Protocol
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The MDA, SOD, GPx and GSH were estimated in liver homogenates using colorimetric methods (spectrophotometer MARCEL S350 PRO). The GPx and SOD activities were assayed using the RANSEL and RANSOD kit, respectively (Randox Laboratories, Crumlin, UK). MDA and GSH concentrations were determined with the BIOXYTECH-MDA-586 and BIOXYTECH GSH-400 kit, respectively (OxisResearch, USA), according to the manufacturer's instructions.
Serum activities of ALT and AST and protein concentration in homogenates were assayed using a commercial enzymatic method in a certified laboratory. The SOD and GPx activities were expressed per milligram of protein. Activities of ALT and AST were expressed per gram of liver.
Serum activities of ALT and AST and protein concentration in homogenates were assayed using a commercial enzymatic method in a certified laboratory. The SOD and GPx activities were expressed per milligram of protein. Activities of ALT and AST were expressed per gram of liver.
10
Oxidative Stress Biomarkers in Brain Regions
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