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2 protocols using anti phospho rb at ser807 811

1

Antibody Acquisition for EMT Analysis

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Anti-vimentin, anti-Snail, and anti-Slug antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-NUCB2, anti-Twist1, and anti-Ki-67 antibodies were obtained from Abcam (Cambridge, MA, USA). Anti-E-cadherin, anti-p21waf1, anti-BCL2, and anti-cyclin D1 antibodies were from Dako (Copenhagen, Denmark). Anti-p27kip1, anti-Rb, anti-X-linked inhibitor of apoptosis (XIAP), anti-BAX, and anti-N-cadherin antibodies were from BD Biosciences (San Jose, CA, USA). Anti-ZEB1 and anti-β-actin antibodies and adriamycin (ADR: D1515) were from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-phospho (p) Rb at Ser807/811, anti-cleaved caspase-3, and anti-poly (ADP-ribose) polymerase 1 (PARP1) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-cyclin A2 and anti-cyclin B1 antibodies were from Novocastra (Newcastle, UK) and Santa Cruz Biotech (Santa Cruz, CA, USA), respectively.
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2

Comprehensive Epithelial-Mesenchymal Transition Antibodies

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Anti-phospho (p) Rb at Ser807/811 (catalog number #9308) and anti-Slug antibodies (#9585) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-EBP50 (ab109430), Anti-cyclin A2 (#ab181591), anti-Snail (#ab180714), and anti-Twist1 (#ab50887) antibodies were obtained from Abcam (Cambridge, MA, USA). Anti-E-cadherin, anti-Ki-67 (#M7240), anti-p21waf1 (#M7202), and anti-cyclin D1 antibodies (#M3635) were from Dako (Copenhagen, Denmark). Anti-p27kip1 (#610242), anti-Rb (#554136), anti-aldehyde dehydrogenase 1 (ALDH1) (#611194), anti-β-catenin (#610154), and anti-N-cadherin antibodies (#610920) were from BD Biosciences (San Jose, CA, USA). Anti-FLAG M2 (#F3165), anti-ZEB1 (#HPA027524), and anti-β-actin antibodies (#HPA025958) were from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-vimentin (sc-6260) and anti-cyclin B1 antibodies (#sc-752) were from Santa Cruz Biotech (Santa Cruz, CA, USA). Anti-E-cadherin antibody (#M106, Takara Bio Inc., Siga, Japan) was purchased from Cell Signaling Technology (Danvers, MA, USA).
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