In vivo f pro

Manufactured by Bruker

Sourced in Germany

The In vivo F-PRO is a laboratory instrument designed for small animal imaging. It provides high-resolution fluorescence imaging capabilities for a variety of pre-clinical research applications.

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Lab products found in correlation

Scid beige mice, by Charles River Laboratories (1 mentions) Renilla luciferase substrate, by Promega (1 mentions) In vivo extreme 4mp, by Bruker (1 mentions) In vivo xtreme 4mp, by Bruker (1 mentions) Rediject coelenterazine h, by PerkinElmer (1 mentions) D luciferin, by GoldBio (1 mentions)

Close spelling variants of this product

In-Vivo F PRO imaging system (15 citations) In-Vivo F PRO system (3 citations)

4 protocols using in vivo f pro

Hepatocellular Injury Model in Immunodeficient Mice

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SCID Beige mice (Charles River) were chosen as animal model. These mice are characterized by severe immunodeficiency affecting B and T lymphocytes, and natural killer cells.
To evaluate the intrahepatic diffusion of cells after transplant, 1.0 × 10⁶ untreated or AEM1-treated cells were labeled with DIR immediately before intrasplenic injection^{47 (link)}. Migration of transplanted cells from the spleen to the liver was detected after 30 min and after 24 h by the In vivo F-PRO (Bruker). The presence of transplanted HepaRG cells was also evaluated in the explanted spleen and liver after 4 weeks.
The day after HepaRG cell transplantation, liver injury was induced by administration of an anti-Fas monoclonal Ab (Jo2/CD95, BD Bioscences), once weekly for 4 weeks. Mice were divided in the following groups:

Controls, transplanted with vehicle (n = 4);

Transplanted with untreated HepaRG cells (n = 4);

Transplanted with AEM1-treated HepaRG cells (n = 4).

At the end of the fourth week, animals were killed, the blood drawn, and the livers excised to be frozen at −80 °C; some liver samples were fixed in 4% formalin for histopatology and immunohistochemistry. Hepatocellular necrosis was assessed by measuring the serum level of AST and ALT, respectively.

Bellanti F., di Bello G., Iannelli G., Pannone G., Pedicillo M.C., Boulter L., Lu W.Y., Tamborra R., Villani R., Vendemiale G., Forbes S.J, & Serviddio G. (2021). Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation. NPJ Regenerative Medicine, 6, 28.

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In Vivo Bioluminescence Imaging of rOC43-ns2DelRluc Replication in Mice

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rOC43-ns2DelRluc replication in mice was evaluated by BLI with an In Vivo F Pro (Bruker Daltonik, Bremen, Germany). Prior to imaging, rOC43-ns2DelRluc-infected mice were injected intraperitoneally with Renilla luciferase substrate (20 μg/g; Promega, Madison, WI, USA) and anaesthetized with isoflurane. After 5 min, mice were placed on a light-tight camera box stage under continuous anesthesia, imaged (acquisition time: 4 min), and the signals were quantified using Bruker molecular imaging software (Bruker).

Niu J., Shen L., Huang B., Ye F., Zhao L., Wang H., Deng Y, & Tan W. (2019). Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice. Antiviral Research, 173, 104646.

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Tracking Tumor Xenograft Regression

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Example 9

1×10⁶8505c-FLuc⁺GFP⁺ cells were injected into NSG mice via tail vein. 2-3×10⁶primary human T cells were injected via tail vein 7-15 days after tumor cell injection. Luminescence imaging of tumor xenografts in live mice was performed using a whole body optical imager (In-Vivo Extreme 4MP, Bruker). Mice were first anesthetized with 3% isoflurane at 2 L/min O₂and subsequent to this, maintained at 2% isoflurane at 2 L/min O₂. Growth or reduction in tumor burden was estimated by integration of luminescence over the lungs or the entire mouse body. Ex-vivo fluorescence imaging of mouse liver, lungs, spleen and resected tumors were performed using a whole body optical imager (In-Vivo F Pro, Bruker).

US11634471B2. Transduced T cells expressing human SSTR2 and application thereof (2023-04-25). Cornell University [US]. Inventors: Moonsoo Jin [US].

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Xenograft ATC Model for Metastasis

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All animal studies were approved by Weill Cornell Medicine’s Institutional Animal Care and Use Committee. 8–10-week-old female and male NOD-scidIL2Rg^null (NSG) mice (Jackson laboratory) were used for xenograft experiments with ATC cells. GFP and fLuc-expressing 8505C (0.75×10⁶ cells per mouse) and patient-derived ATC cells (0.36×10⁶ cells per mouse) were i.v. injected to establish systemic metastasis establishment (28 (link)). Longitudinal measurements of 8505C tumor burdens were taken using a whole-body optical imager (In-Vivo Xtreme 4MP, Bruker) 15 minutes after intraperitoneal injection of 100 µl of 150 µg/ml D-luciferin (GoldBio). Total tumor burden was measured by generating full body region of interests (ROIs) and integrating total flux of luminescence using Bruker Analysis software. GFP-expressing livers, spleen, heart, and lungs were extracted from mice and imaged with a whole-body optical imager (In-vivo F-Pro, Bruker). To track ICAM-1-CAR T cells expressing rLuc, mice were injected i.v. with 100 µl of RediJect Coelenterazine H (Perkin Elmer) and then immediately imaged using the In-Vivo Xtreme 4MP (Bruker).

Min I.M., Shevlin E., Vedvyas Y., Zaman M., Wyrwas B., Scognamiglio T., Moore M.D., Wang W., Park S., Park S., Panjwani S., Gray K.D., Tassler A.B., Zarnegar R., Fahey TJ I.I.I, & Jin M.M. (2017). CAR T therapy targeting ICAM-1 eliminates advanced human thyroid tumors. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(24), 7569-7583.

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