Dmi8 thunder imager 3d

The histological samples were analyzed in triplicate (n = 3) using the LED microscope LEICA DMi8 THUNDER imager 3D composed by camera K5.
For each biological replicate, the entire sections were acquired by LASX Tilescan technology and quantified using LASX 3.7.1 software. Representative images at 63X magnification were acquired. Signal quantification was performed considering the Sum intensity of area covered by fluorescence signal; the mean and standard deviation of biological replicates were calculated.

Hematoxylin and eosin (H&E) staining is based on acidic/basic properties of the chemicals: the hematoxylin stains cells blue whereas the eosin stains the extracellular matrix and cytoplasm pink. H&E staining was performed on both HO2E tissues series and on a human oesophagus biopsy obtained from AMSBIO (Abingdon, UK). Specifically, the biopsy derived from a healthy Caucasian female, 40 years old (specimen ID: CU2008/20 2C-19 A).
The slides were deparaffinized, rehydrated, stained with H&E, dehydrated and finally mounted with organo-limonene and cover slips.
The tissues were evaluated by light microscopy using microscope LEICA DMi8 THUNDER imager 3D composed by camera DFC450 C. The entire (100%) sections were acquired by LASX Tilescan (20x objective).