Dodecyl thiomaltopyranoside

Manufactured by Anatrace

Dodecyl thiomaltopyranoside is a non-ionic detergent used for the solubilization and stabilization of membrane proteins. It is a derivative of maltose with a dodecyl (C12) alkyl chain attached to the reducing end of the sugar.

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Lab products found in correlation

Syprored protein gel stain, by Thermo Fisher Scientific (1 mentions) Soy l α phosphatidylinositol, by Avanti Polar Lipids (1 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Benzamidine, by Thermo Fisher Scientific (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylethanolamine, by Avanti Polar Lipids (1 mentions)

2 protocols using dodecyl thiomaltopyranoside

Fluorescent Lipid and Protein Analysis

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N-(5-Dimethylaminonaphthalene-1-sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (dansyl DHPE) and SYPRO
Red Protein Gel Stain was purchased from Invitrogen Life Technologies
(Grand Island, NY). 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
(POPE), soy l-α-phosphatidylinositol (PI), and ovine
wool cholesterol (Chol) were purchased from Avanti Polar Lipids (Alabaster,
AL). Dodecyl thiomaltopyranoside was purchased from Anatrace (Maumee,
OH). All other materials were purchased from Fisher Scientific (Pittsburgh,
PA). Unless otherwise noted, the buffer solution used for all experiments
contained 50 mM Tris, 600 mM HEPES, 300 mM NaCl, 0.5 mM EDTA, 1 mM
DTT, 4 mM benzamidine, and 24.7 μM Dodecyl thiomaltopyranoside
(elution buffer).

Hall L., Donovan E., Araya M., Idowa E., Jiminez-Segovia I., Folck A., Wells C.D, & Kimble-Hill A.C. (2019). Identification of Specific Lysines and Arginines That Mediate Angiomotin Membrane Association. ACS Omega, 4(4), 6726-6736.

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Lipid Composition for Membrane Studies

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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE), soy l-α-phosphatidylinositol (PI), 1-oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-2-propionyl)amino)-hexanoyl)-sn-glycero-3-phosphoinositol (TopFluor TMR PI), 1-oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-2-propionyl)amino)-hexanoyl)-sn-glycero-3-phosphatidylethanolamine (TopFluor TMR PE), and 1-palmitoyl-2-{12-[(7-nitro-2–1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphatidylcholine (NBD PC) were purchased from Avanti Polar Lipids (Alabaster, AL). Buffer components tris(hydroxymethyl)-aminomethane (Tris), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium chloride, ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), and benzamidine were purchased from Fisher Scientific (Pittsburgh, PA). Dodecyl thiomaltopyranoside was purchased from Anatrace (Maumee, OH). The buffer solution used for all experiments contained 50 mM Tris, 600 mM HEPES, 300 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 4 mM benzamidine, and 24.7 μM Dodecyl thiomaltopyranoside (pH 8.0) (referred to as Amot ACCH domain elution buffer).

Kimble-Hill A.C., Petrache H.I., Seifert S, & Firestone M.A. (2018). Reorganization of Ternary Lipid Mixtures of Nonphosphorylated Phosphatidylinositol Interacting with Angiomotin. The journal of physical chemistry. B, 122(35), 8404-8415.

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