Anti guinea pig igg a7289

Cortex samples were prepared using standard techniques and probed using the following primary antibodies: GFAP (MAB360, 1:2,000; Millipore, Bedford, MA)(Buckman, et al., 2013 (link)), Neprilysin/CD10 (Ab79423, 1:1,000), Receptor for Advanced Glycation End Products (AGEs), RAGE (Ab37674, 1:1,000), Tau-5 (ab80579, 1:400) and phospo-Tau (S396; ab109390, 1:5,000) all from Abcam (Cambridge, MA). TNFa (D2D4, 1:500-1:1,000), and Synaptophysin (D35E4, 1:1,000) Cell Signaling (Danvers, MA), and Actin (D35E4, 1:400-1,5,000; Santa Cruz Biotechnology, Dallas Texas). Appropriate secondary antibodies were selected from Anti-Goat IgG (A5420, 1:5,000), Anti-Guinea Pig IgG (A7289, 1:5,000) and Anti-Rabbit IgG (A0545, 1:5,000) all from Sigma-Aldrich (USA).

Cortical tissue was homogenized using RIPA buffer (Sigma Aldrich, USA) with protease inhibitors (cOmplete protease inhibitor cocktail, Roche, Switzerland). Gels were loaded with 10 μg of protein and membranes were prepared using the iBlot system (LifeTechnologies, USA). Membranes were incubated with GLT-1 (AB1783, 1:4,000) and GFAP (MAB360, 1:2,000; both Millipore, Bedford, MA), xCT (NB-300–318, Novus Biologicals, Littleton, CO, USA), and Actin (D35E4, 1:5,000; Santa Cruz, USA). Appropriate secondary antibodies were selected from Anti-Goat IgG (A5420, 1:5,000), Anti-Guinea Pig IgG (A7289, 1:5,000) and Anti-Rabbit IgG (A0545, 1:5,000) all from Sigma-Aldrich (USA)