Methacrylic acid (99%) (MAA), titanium (IV) isopropoxide (97%) and zirconium (IV) propoxide in n-propanol (70 wt.%) were purchased from Merck and n-propanol from Alfa Aesar and were used as received. Acrylamide (Aam), 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), Lysil-lysine (Lys-Lys), Methacrylic acid (MAA), N-tert-butylAcrylamide (TBAm), N,N′-methylene bisAcrylamide (BIS), N,N,N′,N′-tetramethyl ethylenediamine (TEMED), ammonium persulfate (APS), sodium dodecyl sulfate (SDS), Tris (hydroxymethyl)-aminomethane (TRIS), sodium dihydrogen phosphate, disodium monohydrogen phosphate, hydrochloric acid, sodium hydroxide, sodium chloride, Tween-20, 3-Aminopropyl)Triethoxysilane, acetonitrile, acetic acid, ethanol, were from Sigma–Aldrich (Darmstadt, Germany). The proteins human serum transferrin (HTR), horseradish peroxidase (HRP), trypsin were from Sigma–Aldrich (Darmstadt, Germany). A 40% w/w APS stock solution was freshly prepared prior to polymerization.
Methacrylic acid
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Canada, Spain, France
Methacrylic acid is a colorless, flammable liquid with a pungent odor. It is a carboxylic acid with the chemical formula CH3C(CH3)COOH. Methacrylic acid is used as a raw material in the production of various polymers and copolymers.
Automatically generated - may contain errors
Lab products found in correlation
Ethylene glycol dimethacrylate, by Merck Group (30 mentions)
Methanol, by Merck Group (23 mentions)
Acetonitrile, by Merck Group (23 mentions)
Ethanol, by Merck Group (18 mentions)
Ammonium persulfate, by Merck Group (18 mentions)
Acetic acid, by Merck Group (17 mentions)
Sodium hydroxide (naoh), by Merck Group (16 mentions)
N n methylenebisacrylamide, by Merck Group (15 mentions)
Azobisisobutyronitrile, by Merck Group (15 mentions)
Hydrochloric acid (hcl), by Merck Group (13 mentions)
Methyl methacrylate, by Merck Group (13 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (11 mentions)
Acrylamide, by Merck Group (10 mentions)
2 hydroxyethyl methacrylate, by Merck Group (10 mentions)
Toluene, by Merck Group (9 mentions)
Acetone, by Merck Group (8 mentions)
N isopropylacrylamide, by Merck Group (8 mentions)
Chitosan, by Merck Group (8 mentions)
Potassium persulfate, by Merck Group (8 mentions)
Benzoyl peroxide, by Merck Group (8 mentions)
192 protocols using methacrylic acid
1
Synthesis of Protein-Functionalized Hydrogels
2
Purification and Release Studies of Monomers
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Sodium dodecyl sulfate (SDS), theophylline (THEO), caffeine (CAFF), methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), ammonium persulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate were purchased from Sigma-Aldrich (Milan, Italy).
In the aim to remove stabilizer and impurities, methacrylic acid was purified before use by a single-step passage through an alumina column.
All solvents were reagent or HPLC grade and supplied by VWR (Milan, Italy).
Dialysis membranes of 6–27/32” Medicell International LTD (MWCO: 12–14,000 Da) were employed for the in vitro release studies.
In the aim to remove stabilizer and impurities, methacrylic acid was purified before use by a single-step passage through an alumina column.
All solvents were reagent or HPLC grade and supplied by VWR (Milan, Italy).
Dialysis membranes of 6–27/32” Medicell International LTD (MWCO: 12–14,000 Da) were employed for the in vitro release studies.
3
Synthesis of Cardanol-based Polymers
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The monomers, styrene (St) and methacrylic acid (MAA), as well as the ligands, pentamethyldiethylenetriamine (PMDETA) and bypyridine (dNBpy), initiator ethyl-2-bromo isobutyrate (EBriB), metal catalyst Cu(I)Br, ethanol, glacial acetic acid and diethyl ether were purchased from Sigma Aldrich, St. Louis, MO, USA and used as such. Hydrogenated cardanol (pentadecylphenol, PDP) was kindly supplied by Cardolite Specialty Chemical LLP, Mangaluru, India. Methacryloyl chloride was prepared from methacrylic acid and benzoyl chloride which are purchased from Sigma-Aldrich, St. Louis, MO, USA and used as such. Triethylamine (TEA) was purchased from Spectrochem, Mumbai, India. Styrene monomer was purified by washing with aqueous (5%) NaOH solution, deionised water and finally distilled under reduced pressure and stored at −18 °C. The metal catalyst, Cu(I)Br, was purified by stirring with glacial acetic acid overnight, washed with ethanol and diethyl ether, filtered and dried at 50 °C under vacuum for 6 h.
4
Synthesis of Magnetic Polymer Composites
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Divinylbenzene (DVB) and methacrylic acid (MA) were obtained from Sigma-Aldrich. Ethyl acetate (EtoAc), FeCl3·6H2O, FeSO4·6H2O, glycol, NaOH, HCl and MB were supplied by Tianjin Bodi Chemical Co., Ltd., China. 2,2′-azobis(2-methylpropionitrile) (AIBN) was purchased from Shanghai No.4 Reagent & H.V. Chemical Co., Ltd., China. All of these chemicals were of analytical grade and used as received.
5
Polymeric Blood Oxygenator Membrane Synthesis
6
Synthesis and Characterization of Magnetic Hydrogels
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N-Isopropyl acrylamide NIPAM , methacrylic acid MA , N,N′-methylenebisacrylamide BIS , ammonium persulfate APS , and magnetic nanoparticles
(MNPs) were purchased from Sigma-Aldrich. Potassium chloride (KCl)
was from POCH, Poland. The 500 nm diameter silica beads were procured
from Fiber Optic Centre. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, anMTT reagent, was ordered from Thermo Fisher
Scientific. Deionized ultrapure Merck Millipore Milli-Q water (18.2
M Ω·cm) was used to prepare all aqueous solutions. All
reagents and solvents were of analytical grade and used as received.
(MNPs) were purchased from Sigma-Aldrich. Potassium chloride (KCl)
was from POCH, Poland. The 500 nm diameter silica beads were procured
from Fiber Optic Centre. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, an
Scientific. Deionized ultrapure Merck Millipore Milli-Q water (18.2
M Ω·cm) was used to prepare all aqueous solutions. All
reagents and solvents were of analytical grade and used as received.
7
Synthesis and Purification of Methacrylated Gelatin
8
Synthesis of Functional Polymeric Nanoparticles
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2-(Dimethylamino)ethyl methacrylate (DMAEM, Sigma-Aldrich), methacrylic acid (MA, Sigma-Aldrich), sodium chloride (NaCl, ≥99.5%, Jin Chemical Pharmaceutical), sodium phosphate dibasic (≥99%, Sigma-Aldrich), sodium phosphate monobasic (≥99%, Sigma-Aldrich), 2-cyano-2-propyl benzodithioate (Aldrich), azobisisobutyronitrile (AIBN, Aldrich), diethyl ether (Sigma-Aldrich), N,N-dimethylformamide (DMF, Aldrich), dithiothreitol (DTT, Sigma-Aldrich), fluorescein diacetate (FDA, Sigma), acetone (≥99.8%, Merck), l -glutathione reduced (GSH, Sigma-Aldrich), rhodamine red® C2 maleimide (rhodamine-maleimide, Life Technology), doxorubicin hydrochloride (Dox, Ontario Chemical Inc.), cetyltrimethylammonium bromide (CTAB, Aldrich), triethanolamine (Sigma), tetraethyl orthosilicate (TEOS, Aldrich), glycerol (Sigma), Dulbecco's modified Eagle's medium (DMEM, Welgene), penicillin-streptomycin (Welgene), 0.25% trypsin-EDTA (Welgene), fetal bovine serum (FBS, Welgene), yeast extract-peptone-dextrose (YPD, Duchefa-Biochemie), lyticase from Arthrobacter luteus (lyticase, ≥ 2000 units per mg protein, Sigma), and Tris–EDTA (TE) buffer solution (pH 7.5, Intron Biotechnology) were used as received. 2-(Pyridyl disulfide)ethyl methacrylate (PDEM) was synthesized by following a previously reported procedure.13 Deionized water (DI water, 18.3 MΩ cm) from the Human Ultrapure System (Human Corp.) was used.
9
Synthesis and Characterization of Chitosan Nanomaterials
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Chitosan (CS) (MW = 70 kDa, degree of deacetylation of 90%) was purchased from Vietnamese Chitosan Co., Ltd. Methacrylic acid (MAA), potassium persulfate (K2S2O8), and acetic acid (CH3COOH) were purchased from Merck, Germany. Glycerol and CaCl2 were purchased from Xilong, China and BHI medium was purchased from Sigma–Aldrich, Saint Louis, MO, USA.
The pectin (P) was extracted from Tiliacora triandra using a heating method [15 ]. The degree of esterification of pectin was 48.36 and its molecular weight was 111.1 kDa.
Nanochitosan (NCS) were prepared by polymerizing Methacrylic acid (MAA) in chitosan (CS) solution according to the method described by Nguyet and de Moura [5 (link),16 ,17 ]. Firstly, chitosan was dissolved in a 0.5% MMA solution for 12 h. The chitosan concentration used in the synthesis was 0.8 w/w%. K2S2O8, with a concentration of 0.6 mmol was then added to the chitosan—methacrylic solution at 70°C for 1 h under stirring. After that, the mixture was cooled at 4 °C. The suspension was centrifuged at 4000 r/min for 30 min and a suspension was obtained [5 (link)]. Chitosan nanoparticles were twice washed in distilled water, producing a chitosan nanoparticles suspension (NCS).
The pectin (P) was extracted from Tiliacora triandra using a heating method [15 ]. The degree of esterification of pectin was 48.36 and its molecular weight was 111.1 kDa.
Nanochitosan (NCS) were prepared by polymerizing Methacrylic acid (MAA) in chitosan (CS) solution according to the method described by Nguyet and de Moura [5 (link),16 ,17 ]. Firstly, chitosan was dissolved in a 0.5% MMA solution for 12 h. The chitosan concentration used in the synthesis was 0.8 w/w%. K2S2O8, with a concentration of 0.6 mmol was then added to the chitosan—methacrylic solution at 70°C for 1 h under stirring. After that, the mixture was cooled at 4 °C. The suspension was centrifuged at 4000 r/min for 30 min and a suspension was obtained [5 (link)]. Chitosan nanoparticles were twice washed in distilled water, producing a chitosan nanoparticles suspension (NCS).
10
Extraction and Characterization of Bioactive Compounds
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All chemicals used in the research work were of analytical grade. TRD was purchased from Searle (Pvt.) Ltd., Karachi, Pakistan. Aloe vera gel was extracted from plant leaves. Tamarind seed powder was purchased from BDH, England. Methacrylic acid, potassium dihydrogen phosphate, potassium chloride, and ethanol were purchased from Merck, Germany. Ammonium persulfate, methylene-bis-acrylamide, hydrochloric acid, and sodium hydroxide were purchased from Sigma-Aldrich, United States.
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