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Gbl103250

Manufactured by Merck Group
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Sourced in United States, Macao

The GBL103250 is a laboratory equipment product by Merck Group. It is designed for general laboratory use. No further details about its core function can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Onemp instrument, by Refeyn (4 mentions) 50 mm microscope coverslips, by Thermo Fisher Scientific (4 mentions) Acquiremp, by Refeyn (3 mentions) Lc0725, by Thermo Fisher Scientific (1 mentions) Acquiremp software, by Refeyn (1 mentions) Discovermp software, by Refeyn (1 mentions)

4 protocols using gbl103250

1

Mass Photometry Analysis of rAAV Samples

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The MP experiments were performed at room temperature using the OneMP instrument (Refeyn, Oxford, UK) following the standard protocol [35 (link)]. The 24×50 mm microscope coverslips (Fisher Scientific, Waltham, MA, USA) were prepared by cleaning with MilliQ water and isopropanol, and drying under a stream of clean nitrogen, as described previously [36 (link)]. A piece of clean, precut 2×2-well culturewell gasket (GBL103250, Sigma, MO, USA) was attached to the coverslip. The rAAV stocks’ concentrations were measured by the UV absorbance and diluted in PBS to a concentration of about 1011 virus particles per milliliter. This concentration was high enough to provide a high frequency of landing events without producing a large number of landing event overlaps that would degrade the data quality (Supplementary Table S1) [35 (link)]. All samples and stock solutions were carefully re-mixed just before use to assure sample homogeneity. Ten microliters of the filtered PBS buffer were loaded into a well of the culturewell gasket, and, after MP focusing, a 10 μl of rAAV sample was added into the same well. Immediately after the solution was mixed by pipetting, a 2 min video was recorded using the AcquireMP (Refeyn, Oxford, UK) software.
Wu D., Hwang P., Li T, & Piszczek G. (2022). Rapid characterization of adeno-associated virus (AAV) gene therapy vectors by mass photometry. Gene therapy, 29(12), 691-697.
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2

Biomass Quantification of Q-beta Conjugates

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The 24 × 50 mm microscope coverslips (Fisher Scientific, Waltham, MA) and precut 2 × 2 silicon gasket wells (GBL103250, Sigma, MO) were cleaned and assembled as described in the literature.46 (link) Measurements were performed on OneMP instrument (Refeyn, Oxford, UK) at room temperature. PBS (1×, pH 7.2) buffer was filtered through 0.22 μM filters before use. Ten microliters of the buffer were loaded in gasket well to focus the objective on the coverslip surface. Qβ conjugates stock were diluted 100 times in PBS (1×, pH 7.2) buffer and added to buffer in the well. The MP video was immediately recorded after the sample loading using the AcquireMP software (Refeyn, Oxford, UK). A 1 min video was recorded for each sample, and each sample was repeated twice. Data were processed using the DiscoverMP software (Refeyn, Oxford, UK) with the threshold filter values of 5. The mass distribution was plotted as histograms with bin width of 50 kDa and fit with Gaussian peaks to obtain the average mass of different species. The contrast-to-mass calibration was performed using an unstained protein ladder (LC0725, Thermo Fisher, Wattham, MA) and empty AAV5 sample (Virovek, Hayward, CA).
Rashidijahanabad Z., Kelly M., Kamruzzaman M., Qadri F., Bhuiyan T.R., McFall-Boegeman H., Wu D., Piszczek G., Xu P., Ryan E.T, & Huang X. (2022). Virus-like Particle Display of Vibrio cholerae O-Specific Polysaccharide as a Potential Vaccine against Cholera. ACS infectious diseases, 8(3), 574-583.
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3

Mass Photometry Analysis of rAAV Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The MP experiments were performed at room temperature using the OneMP instrument (Refeyn, Oxford, UK) following the standard protocol [35 (link)]. The 24×50 mm microscope coverslips (Fisher Scientific, Waltham, MA, USA) were prepared by cleaning with MilliQ water and isopropanol, and drying under a stream of clean nitrogen, as described previously [36 (link)]. A piece of clean, precut 2×2-well culturewell gasket (GBL103250, Sigma, MO, USA) was attached to the coverslip. The rAAV stocks’ concentrations were measured by the UV absorbance and diluted in PBS to a concentration of about 1011 virus particles per milliliter. This concentration was high enough to provide a high frequency of landing events without producing a large number of landing event overlaps that would degrade the data quality (Supplementary Table S1) [35 (link)]. All samples and stock solutions were carefully re-mixed just before use to assure sample homogeneity. Ten microliters of the filtered PBS buffer were loaded into a well of the culturewell gasket, and, after MP focusing, a 10 μl of rAAV sample was added into the same well. Immediately after the solution was mixed by pipetting, a 2 min video was recorded using the AcquireMP (Refeyn, Oxford, UK) software.
Wu D., Hwang P., Li T, & Piszczek G. (2022). Rapid characterization of adeno-associated virus (AAV) gene therapy vectors by mass photometry. Gene therapy, 29(12), 691-697.
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4

MP Analysis of Recombinant AAV Samples

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The MP experiments were performed at room temperature using the OneMP instrument (Refeyn, UK). The 24×50 mm microscope coverslips (Fisher Scientific, Waltham, MA) were prepared by cleaning with MilliQ water and isopropanol, and drying under a stream of clean nitrogen, as described previously [37] . A piece of clean, precut 2×2-well culturewell gasket (GBL103250, Sigma, MO) was attached to the coverslip. The rAAV stocks were diluted in PBS to a concentration of about 10 11 virus particles per milliliter. Ten microliters of the filtered PBS buffer were loaded into a well of the culturewell gasket, and, after MP focusing, a 10 µL of rAAV sample was added into the same well. Immediately after the solution was mixed by pipetting, a 2-minute video was recorded using the AcquireMP (Refeyn, UK) software.
Wu D., Hwang P., Li T., & Piszczek G. (2021). Rapid Characterization of AAV gene therapy vectors by Mass Photometry.
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