HMWC with approximately 2,000 kDa, glucosamine hydrochloride (GlcN) and N-acetyl glucosamine (GlcNAc) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trypan blue and chitosanase from Streptomyces griseus were purchased from Sigma Chemical Co. Potato dextrose broth (PD) was purchased from Difco™ (Becton Dickinson, Cockeysville, MD, USA). MTT, WST, thiabendazole and imazalil were also purchased from Sigma Chemical Co. All other reagents were used without further purification and were of the highest grade available.
Glucosamine hydrochloride glcn
Manufactured by Merck Group
Sourced in United States, Germany
Glucosamine hydrochloride (GlcN) is a naturally occurring compound that is a precursor to glycosaminoglycans, a class of molecules that are important structural components of cartilage. GlcN is commonly used as a dietary supplement and in various laboratory applications.
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Lab products found in correlation
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Thiabendazole, by Merck Group (1 mentions)
N acetylglucosamine glcnac, by Merck Group (1 mentions)
Chitosanase from streptomyces griseus, by Merck Group (1 mentions)
Imazalil, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Sodium dihydrogen phosphate monohydrate, by Merck Group (1 mentions)
2 protocols using glucosamine hydrochloride glcn
1
Characterization of High-Molecular-Weight Chitosan
2
Chondroitin and Hyaluronic Acid Analysis
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The investigated saccharides (shown in Fig. 1 ), namely glucosamine hydrochloride (GlcN), glucuronic acid (GlcA), GlcNAc and unsaturated chondroitin disaccharide Δdi-0S sodium salt (C0S) were purchased from Sigma-Aldrich (Taufkirchen, Germany) in the highest available purity and used without any further purification. The tetrasaccharide of hyaluronic acid (HA4) was a generous gift by Jörg Rademann (FU Berlin) and prepared as recently described [24 (link)].Fig. 1 ![]()
A stock solution of NaOCl was prepared in water and its concentration spectrophotometrically determined at pH 12 (ε290 = 350 L mol−1 cm−1) each day prior to use [25 (link)]. The NaOCl stock solution was diluted to the desired working concentration with either phosphate buffer or sodium acetate buffer.
Survey of the different mono- and oligosaccharides, which were investigated in this study.
A stock solution of NaOCl was prepared in water and its concentration spectrophotometrically determined at pH 12 (ε290 = 350 L mol−1 cm−1) each day prior to use [25 (link)]. The NaOCl stock solution was diluted to the desired working concentration with either phosphate buffer or sodium acetate buffer.
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