Ultrapure water was from the Milli‐Q‐system (Millipore SA, Molsheim, France). Acetonitrile with HPLC grade was from Panreac (Barcelona, Spain); hexane, formic acid and ethanol were from Sigma‐Aldrich (Steinheim, Germany). Phenolic standards were tyrosol (> 99.5%) and oleocanthal from Sigma‐Aldrich, oleacein from Phytolab (Vestenbergsgreuth, Germany), luteolin‐7‐O‐glucoside (> 98%), verbascoside (> 99%), oleuropein (> 98%) and rutin (> 99%) from Extrasynthese (Genay, France). 4‐Methyl‐2‐pentanol (≥ 98.0%) used as internal standard and the 73 external standard for analysis of volatile organic compounds (VOCs) were from Sigma‐Aldrich: their purity was previously reported.24 Solutions of VOCs were prepared in refined olive oil free from VOCs.
Luteolin 7 o glucoside
Manufactured by Extrasynthese
Sourced in France, Germany
Luteolin-7-O-glucoside is a naturally occurring flavonoid compound. It functions as an antioxidant and exhibits various biological activities. This compound is commonly used in research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Apigenin 7 o glucoside, by Extrasynthese (27 mentions)
Apigenin, by Extrasynthese (17 mentions)
Luteolin, by Extrasynthese (17 mentions)
Formic acid, by Merck Group (16 mentions)
Rutin, by Extrasynthese (12 mentions)
Quercetin, by Extrasynthese (12 mentions)
Caffeic acid, by Extrasynthese (12 mentions)
Rosmarinic acid, by Extrasynthese (10 mentions)
Methanol, by Merck Group (10 mentions)
Verbascoside, by Extrasynthese (9 mentions)
Oleuropein, by Extrasynthese (9 mentions)
Acetonitrile, by Merck Group (9 mentions)
Kaempferol 3 o glucoside, by Extrasynthese (8 mentions)
Isorhamnetin 3 o glucoside, by Extrasynthese (8 mentions)
Gallic acid, by Merck Group (8 mentions)
Chlorogenic acid, by Extrasynthese (7 mentions)
Luteolin 4 o glucoside, by Extrasynthese (6 mentions)
Isoquercitrin, by Extrasynthese (6 mentions)
Kaempferol 3 o rutinoside, by Extrasynthese (6 mentions)
Quercetin 3 o glucoside, by Extrasynthese (6 mentions)
55 protocols using luteolin 7 o glucoside
1
HPLC-MS Analysis of Olive Oil Phenolics
2
Identification of Bioactive Compounds
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Choline chloride, L-(+)-lactic acid, glycerol, 1, 2-propanediol were purchased from Sigma-Aldrich (Steinheim, Germany). Citric acid and L-proline were purchased from Merck (Darmstadt, Germany). Verbascoside, luteolin 7-O-glucoside, apigenin 7-O-glucoside and gallic acid commercial standards of HPLC grade (>99%) (Extrasynthese, Genay, France) were used as references for identification. Methanol, ethanol, chloroform, ethyl acetate, n-butanol, methyl tert-butyl ether and acetonitrile were of HPLC quality. Formic acid was of analytical grade (>99%).
3
Flavonoid Extraction and Quantification
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All reagents and solvents for extraction, as well as UPLC and HPLC-MS analyses (methanol, acetonitrile, formic acid, dimethylsulfoxide, polyamide and chloroform), were from Sigma-Aldrich (Poznań, Poland), and ultrapure water was obtained from a Millipore Direct Q3 device. Standards of flavonoids (apigenin, apigenin-7-O-glucoside, apigenin-6-C-glucoside, apigenin-8-C-glucoside, isorhamnetin, luteolin, luteolin 7-O-glucoside, luteolin 4′-O-glucoside, luteolin-8-C-glucoside, luteolin-6-C-glucoside, quercetin 3-O-galactoside, quercetin, rutin, isorhamnetin, isorhamnetin 7-O-glucoside) were purchased from Extrasynthese (Genay, France).
Extracts were prepared according to [21 (link)]. Weighted samples of frozen leaves (about 100 mg) were placed in Eppendorf tubes with 1.4 mL of 80% methanol and the internal standard, homogenized using a ball mill (MM 400, Retsch, Haan, Germany) and subsequently placed in an ultrasonic bath for 30 min and centrifuged (11,000× g) for 10 min. apigenin (5 µL of 1 mg/mL solution in dimethylsulfoxide) was used as the internal standard in extracts prepared for the UPLC analyses. Samples of the secondary metabolite extract were directly subjected to HPLC-MS analysis and, prior to the UPLC analysis, 200 µL were diluted with 80% methanol (800 µL).
Extracts were prepared according to [21 (link)]. Weighted samples of frozen leaves (about 100 mg) were placed in Eppendorf tubes with 1.4 mL of 80% methanol and the internal standard, homogenized using a ball mill (MM 400, Retsch, Haan, Germany) and subsequently placed in an ultrasonic bath for 30 min and centrifuged (11,000× g) for 10 min. apigenin (5 µL of 1 mg/mL solution in dimethylsulfoxide) was used as the internal standard in extracts prepared for the UPLC analyses. Samples of the secondary metabolite extract were directly subjected to HPLC-MS analysis and, prior to the UPLC analysis, 200 µL were diluted with 80% methanol (800 µL).
4
Comprehensive Analysis of Phenolic Compounds
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Water, methanol, acetonitrile, and formic acid (Fisher Scientific, Fair Lawn, NJ, USA) were of Optima® LC/MS grade; ascorbic acid (Panreac, Barcelona, Spain), analytical grade; and glacial acetic acid (Merck, Darmstadt, Germany), Suprapur® quality. Leucine Enkephalin acetate hydrate and sodium formate solution were provided by Sigma-Aldrich Chemie (Steinheim, Germany). Luteolin-7-O-glucoside, kaempferol-3-O-glucoside, quercetin-3-O-galactoside, quercetin-3-Orhamnoside were purchased from Extrasynthèse (Genay, France); caffeoyltartaric acid and quercetin-3-O-glucoside, from Chromadex (Irvine, CA, USA); 5-O-caffeoylquinic acid, p-coumaric acid, 1,5-dicaffeoylquinic acid, 1,3-dicaffeoylquinic acid, and quercetin-3-O-rutinoside, from Sigma-Aldrich Chemie (Steinheim, Germany); and ferulic acid, caffeic acid, and 3,4-dihydroxybenzoic acid, from Fluka Chemie (Steinheim, Germany). Standard stock solutions of phenolic compounds were prepared in methanol; and a reference solution of these compounds (5 μg/mL), in methanol-water-acetic acid (30:65:5, v/v/v).
5
Antioxidant and Enzyme Activity Quantification
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All chemicals for antioxidant and enzyme activity were obtained as pure commercial products from Sigma Chemical Co (St. Louis, MO, USA) and used without further purification. HPLC-grade acetonitrile was obtained from Merck KgaA (Darmstadt, Germany) and formic acid was purchased from Prolabo (VWR International, France). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA). The phenolic compounds standards (5-O-caffeoylquinic acid, luteolin-6-C-glucoside, luteolin-7-O-glucoside and apigenin) were from Extrasynthese (Genay, France).
6
Analytical Solvent Extraction and Analysis
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Methanol and ethanol of analytical grade (Fisher Chemical, Loughborough, UK) were used as extraction solvents. Folin-Ciocalteu, gallic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium carbonate, potassium persulfate, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), acetic acid, hydrochloric acid, ferric chloride, acetonitrile, and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (St. Louise, MO, USA). All the organic solvents were HPLC grade. Ultrapure water from a purified system (Isopad Isomantle, Gemini BV, Pr. Beatrixlaan 301, 7312 DG Apeldoorn, The Netherlands) was used. External standards (purity ≥ 99%) caffeic acid, catechin, chlorogenic acid, diosmetin, ferulic acid, glycitin, isorhamnetin, (-)-epicatechin, luteolin-7-O-glucoside, luteolin-4′-O-glucoside, myricetin, naringin, p-hydroxybenzoic acid, quercetin, and rutin were purchased from Extrasynthese (Lyon Nord, Genay Cedex, France). Hispidin (purity ≥ 98%) was purchased from Sigma-Aldrich.
7
Quantification of Phytochemical Standards
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Methanol (MeOH) and acetonitrile (AcN) were purchased from Merck (Darmstadt, Germany) and phosphoric acid from Sigma-Aldrich (St. Louis, MO, USA). Standards of apigenin, apigenin-7-O-glucoside, catechin, hydroxytyrosol, luteolin, luteolin-7-O-glucoside, oleuropein, rutin, tyrosol, and verbascoside were purchased from Extrasynthese (Genay, France). Deionized water was obtained by Siemens UltraClear (Siemens AG, München, Germany).
Multi-element standard solution from Perkin Elmer (NexION Setup Solution, Waltham, MA, USA) was used. Argon used to form plasma for the inductively coupled plasma mass spectrometer (ICP-MS) analysis was of purity 6.0 (Messer, Austria). Acetylene was supplied by Messer (Messer Croatia Plin d.o.o., Zaprešić, Croatia).
Deionized water was obtained by Siemens UltraClear (Siemens AG, München, Germany).
Multi-element standard solution from Perkin Elmer (NexION Setup Solution, Waltham, MA, USA) was used. Argon used to form plasma for the inductively coupled plasma mass spectrometer (ICP-MS) analysis was of purity 6.0 (Messer, Austria). Acetylene was supplied by Messer (Messer Croatia Plin d.o.o., Zaprešić, Croatia).
Deionized water was obtained by Siemens UltraClear (Siemens AG, München, Germany).
8
HPLC-MS Analysis of Sesquiterpene Lactones
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Acetonitrile (hypergrade for LC–MS), formic acid (for LC-MS), and methanol (analytical grade) were purchased from Chromasolv (Bulgaria). The authentic standards gallic, vanillic, protocatechuic, gentisic, salicylic, p-coumaric, rutin, isoquercitrin, luteolin 7-O-rutinoside, luteolin 7-O-glucoside, isorhamnetin 3-O-glucoside, apigenin 7-O-glucoside, luteolin, quercetin, apigenin, and chrysoeriol were obtained from Extrasynthese (Genay, France). Rosmarinic, caffeic, ferulic, chlorogenic, neochlorogenic, 3,4-dicaffeoylquinic, 3,5-dicaffeoylquinic, 1,5-dicaffeoylquinic, and 4,5-dicaffeoylquinic acid were supplied from Phytolab (Vestenbergsgreuth, Germany). Sonchuside A and 8-acetyl-15β-D -glucopyranosyllactucin were previously isolated and identified by Zidorn et al., 2005 [8 (link)].
9
Preparation of Phenolic Compound Standards
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Acetonitrile 99.9% was of HPLC grade from Fisher Scientific (Lisbon, Portugal).
Phenolic compound standards (apigenin-6-C-glucoside, caffeic acid, luteolin-7-Oglucoside, naringenin, quercetin-3-O-rutinoside and rosmarinic acid) were from Extrasynthese (Genay, France). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid) was purchased from Sigma (St. Louis, MO, USA) and 2,2-diphenyl-1picrylhydrazyl (DPPH•) was obtained from Alfa Aesar (Ward Hill, MA, USA). β-Carotene and linoleic acid were acquired from Sigma-Aldrich (St. Louis, Missouri, USA) and Tween 80 from Panreac (Barcelona, Spain). All other solvents and reagents were acquired from scientific retailers. Ferrous ammonium sulfate(II) hexahydrate, sodium chloride and sulfuric acid, all with PA purity, were purchased from Panreac S.A. (Barcelona, Spain), in order to prepare the acid aqueous Fricke dosimeter solution.
Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
Phenolic compound standards (apigenin-6-C-glucoside, caffeic acid, luteolin-7-Oglucoside, naringenin, quercetin-3-O-rutinoside and rosmarinic acid) were from Extrasynthese (Genay, France). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid) was purchased from Sigma (St. Louis, MO, USA) and 2,2-diphenyl-1picrylhydrazyl (DPPH•) was obtained from Alfa Aesar (Ward Hill, MA, USA). β-Carotene and linoleic acid were acquired from Sigma-Aldrich (St. Louis, Missouri, USA) and Tween 80 from Panreac (Barcelona, Spain). All other solvents and reagents were acquired from scientific retailers. Ferrous ammonium sulfate(II) hexahydrate, sodium chloride and sulfuric acid, all with PA purity, were purchased from Panreac S.A. (Barcelona, Spain), in order to prepare the acid aqueous Fricke dosimeter solution.
Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).
10
Phenolic Compound Extraction and Analysis
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Acetonitrile 99.9% was of HPLC grade from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards (apigenin-6-Cglucoside, caffeic acid, chlorogenic acid, hesperetin, luteolin-7-Oglucoside, naringenin, quercetin-3-O-rutinoside and rosmarinic acid) were from Extrasynthese (Genay, France). Fetal bovine serum (FBS), L-glutamine, Hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraAcetic acid), penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, UT, USA). Acetic acid, formic acid, ellipticine, sulforhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris were from Sigma Chemical Co. (St. Louis, MO, USA). Water was treated in Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). Ferrous ammonium sulfate(II) hexahydrate, sodium chloride and sulfuric acid, all with PA purity, were purchased from Panreac S.A. (Barcelona, Spain) (proanalysis), in order to prepare the acid aqueous Fricke dosimeter solution.
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