Usage of archived formalin-fixed, paraffin-embedded tissue block was approved by the Institutional Review Board of MacKay Memorial Hospital. Tissue slides from glioblastoma patients were evaluated by immunohistochemistry and hematoxylin/eosin staining using polyclonal antibody against PON2 (LifeSpan BioSciences) through the avidin-biotin complex method, as described previously [33 (link)]. Immunoreactivity for PON2 was visualized using DAB/nickel substrate (Vector Laboratories, Burlingame, CA).
Dab nickel substrate
Manufactured by Vector Laboratories
Sourced in United States
DAB/nickel substrate is a laboratory reagent used in immunohistochemistry and immunocytochemistry applications. It functions as a chromogenic substrate, producing a brown or black colored reaction product when exposed to the enzyme horseradish peroxidase (HRP).
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Lab products found in correlation
3 protocols using dab nickel substrate
1
Immunohistochemical Analysis of PON2 in Glioblastoma
2
Immunohistochemical Analysis of AGR2 in HCC
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The use of archived formalin-fixed, paraffin-embedded tissue blocks was approved by the Institutional Review Board of National Cheng Kung University Hospital. Tissue slides from HCC patients were evaluated via immunohistochemistry and hematoxylin/eosin staining using a polyclonal antibody against AGR2 (GeneTex, Hsinchu, Taiwan) according to the avidin–biotin complex method, as described previously [31 (link)]. Immunoreactivity for AGR2 was visualized using DAB/nickel substrate (Vector Laboratories, Burlingame, CA).
3
Immunohistochemical Analysis of PTP4A3 in HCC
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Formalin-fixed and paraffin-embedded clinical HCC specimens were evaluated by immunohistochemistry staining using the avidin-biotin complex method, as described previously.28 (link) Sections were air dried and fixed in ice-cold acetone for 10 min, then rehydrated with PBS for 5 min, blocked with protein block (Dako) for 20 min, and incubated with polyclonal antibody against PTP4A3 (GeneTex, Hsinchu, Taiwan) for 1 h at room temperature. Positively stained cells with PTP4A3 immunoreactivity were dark as visualized using DAB/nickel substrate (Vector Laboratories, Burlingame, CA, USA). We examined the PTP4A3 staining intensity using a microscope (Zeiss) equipped with a cooled charge-coupled device camera (Axiocam HRm; Zeiss, Oberkochen, Germany).
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