Rosiglitazone rsg a00183

Rosiglitazone (RSG # A00183, MW: 357.4) was obtained from the Adipogen. Reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad Laboratories (Hercules, CA, USA). The antibodies used in this study were purchased from the various sources: Rabbit anti-ZO-1 (#402200), mouse anti-occludin (#331500) and anti-claudin-5 (#4C3C2) from Life Technologies; rabbit anti-PPARγ (#331500) from Invitrogen; rabbit anti-Nrf2 (#NBP-1-32822) from Novus Biologicals. Mouse anti-PECAM-1 (#sc-376764), mouse anti-NQO-1 (#sc-376023), mouse anti-HO1 (#sc-390991) and mouse anti-NFκB-p65 (#sc-(F-6)-8008) from Santa Cruz Biotechnology. Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) HRP-linked secondary antibodies were obtained from GE Healthcare (Piscataway, NJ, USA).

Reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad Laboratories (Hercules, CA, USA). Quantikine ELISA kits were obtained from R & D systems (Minneapolis, MN, USA), Thermo Fisher Scientific (Waltham, CA, USA), and MyBioSource (San Diego, CA, USA). Additional ELISA kits were purchased from Aviva Systems Biology (San Diego, CA, USA). Pierce BCA Protein Assay Kit (#23225) was purchased from Thermo Fisher Scientific. Gel electrophoresis was performed using Mini-Protean^®TGXTM gels 4–15% (#456–1084) from Bio-Rad Laboratories. Rosiglitazone (RSG # A00183, MW: 357.4) was obtained from Adipogen, and metformin (MF #PHR1084, MW:165.6) was obtained from Sigma-Aldrich.