Luteolin (>99% purity) was purchased from Hangzhou FST Pharmaceutical Co., Ltd. (Hangzhou, China). UW solution was purchased from Bristol-Myers Squibb (New York, NY, USA). Dulbecco's modified Eagle's medium (DMEM) solution, pentobarbital sodium and heparin were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). The calcium florescent detection kits for mitochondria (cat. no. HL10153.1) and sarcoplasmic reticulum (SR; cat. no. GMS10157.1), and intracellular calcium concentration fluorescent detection kits (cat. no. JM325) were purchased from Shanghai Haling Biological Technology Co., Ltd. (Shanghai, China). Fluo-3 acetoxymethyl (AM) was purchased from Beyotime Institute of Biotechnology (Haimen, China). Collagenase type I was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Low Ca2+ enzymatic solution (50 µmol/l) was prepared by dissolving 20 mg collagenase type 1, 2 mg protease E (Sigma-Aldrich; Merck KGaA), 10 mg bovine serum albumin (MP Biomedicals, LLC, Santa Ana, CA, USA) and 72 mmol/l calcium chloride into 30 ml calcium free Tyrode's solution, which was composed of 140 mM sodium chloride, 5 mM potassium chloride, 10 mM HEPES, 2 mM BAPTA, 10 mM glucose, and 10 mM Na pyruvate (pH 7.4±0.2).
Uw solution
Manufactured by Bristol-Myers Squibb
Sourced in United Kingdom
UW solution is a laboratory reagent used for the preservation and transportation of biological samples. It is a balanced salt solution that maintains the physiological environment of cells and tissues, helping to prevent degradation during handling and storage.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Collagenase type 1, by Merck Group (1 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
Heparin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by MP Biomedicals (1 mentions)
Protease e, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Phenylmethanesulfonyl fluoride pmsf, by Merck Group (1 mentions)
Agarose, by Takara Bio (1 mentions)
Control sirnas sc 37007, by Santa Cruz Biotechnology (1 mentions)
Diethylpyrocarbonate depc, by Merck Group (1 mentions)
Revertra ace reverse transcription kit, by Toyobo (1 mentions)
5 protocols using uw solution
1
Calcium Signaling in Isolated Cardiomyocytes
2
Intrahepatic Biliary Epithelial Cell Silencing
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Human intrahepatic biliary epithelial cells (HIBECs) were purchased from ScienCell Research Laboratories (CA, USA). Short-interfering RNAs (siRNAs) specific for Trps1 (sc106642) and control siRNAs (sc37007) were ordered from Santa Cruz Biotechnology (CA, USA). We obtained RPMI 1640, fetal bovine serum (FBS), and trypsin from HyClone (USA). The UW solution was from Bristol-Myers Squibb, Trizol was from Invitrogen, a ReverTra Ace® reverse transcription kit was from TOYOBO, RIPA lysis buffer was from Chongqing Jinmai Technology Co. Ltd (China), and agarose was bought from Takara. We purchased diethylpyrocarbonate (DEPC), phenylmethanesulfonyl fluoride (PMSF), and sodium dodecyl sulfate (SDS) from Sigma–Aldrich (USA).
3
Liver Transplant Donor Tissue Sampling
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Samples were collected from donor livers (n = 10) during orthotopic liver transplantation at St. Vincent's University Hospital. During retrieval, the donor aorta and superior mesenteric vein were flushed with University of Wisconsin (UW) solution (Bristol-Myers Squibb, Uxbridge, UK) at the time of exsanguination. The liver was flushed again with UW solution after excision of the organ until all blood was removed and the perfusate appeared clear, at which time the liver was placed in a container with UW solution and packed on ice for transportation. Donor livers were transplanted within 12 h. At implantation, after completion of the upper inferior cava anastomosis, livers were flushed with normal saline through the portal vein to wash out the UW before reperfusion. This wash-out fluid was collected from the inferior vena cava; the UW transportation solution was also collected. A matched donor blood sample was taken at the time of organ retrieval. Peripheral blood was obtained from anonymised blood donors from the Irish Blood transfusion Board (IBTS). All protocols were approved by St. Vincent's University Hospital Ethics Committee and the Trinity College Dublin School of Medicine Research Ethics Committee, in accordance with the ethical guidelines of the 1975 Declaration of Helsinki.
4
Kidney Preservation Protocols at Varying Temperatures
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The surgical and experimental protocols were performed in accordance with the ARRIVE guidelines, with EU Directive 2010/63/EU, and with institutional Poitou-Charentes Ethical Comity of animal experimentation (protocol number: CE2012-4). We used 3 months old large white pigs weighting 40 ± 4 kg (IBiSA plateforme, INRA, Surgères, France). After analgesia/anesthesia, surgical procedures were performed to remove the right kidney, which was flushed with UW solution (Bristol-Myers-Squibb, France) at various selected temperatures (4°C, 19°C or 32°C) and preserved during 24 hours under static conditions at the selected temperature.
5
Liver Transplant Donor Sample Analysis
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Samples were collected from donor livers (n = 28) during orthotopic liver transplantation at St. Vincent's University Hospital. During retrieval, the donor aorta and superior mesenteric vein were flushed with University of Wisconsin (UW) solution (Bristol-Myers Squibb, Uxbridge, UK) at the time of exsanguination. The liver was flushed again with UW solution after excision of the organ until all blood was removed and the perfusate appeared clear, at which time the liver was placed in a container with UW solution and packed on ice for transportation. Donor livers were transplanted within 12 hours. At implantation, after completion of the upper inferior vena cava anastomosis, livers were flushed with normal saline through the portal vein to wash out the UW before reperfusion. This wash-out fluid was collected from the inferior vena cava; the UW transportation solution was also collected. A matched donor blood sample was taken at the time of organ retrieval. Peripheral blood was obtained from anonymized blood donors from the Irish Blood transfusion Board (IBTS). All protocols were approved by St. Vincent's University Hospital Ethics Committee in accordance with the ethical guidelines of the 1975 Declaration of Helsinki.
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